透射电镜显示土壤腐植酸及其电泳组分随时间的超分子转化

IF 5.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Olga Trubetskaya, Olga Selivanova, Vadim Rogachevsky, Oleg Trubetskoj
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引用次数: 0

摘要

各种环境因素对土壤有机质不同形态形成的影响已被广泛研究,但在溶液中停留的时间如何影响土壤腐殖质形态结构的演变和可能的转变仍不确定。方法采用醋酸铀酰负对比染色的透射电子显微镜(TEM)研究土壤腐殖酸(HAs)在相同溶液中培养150 d前后的形态差异及其个别电泳组分。结果首次发现,在恒定环境条件下,土壤腐殖质样品在150天的培养过程中,可以通过两种完全不同的方式聚集,形成囊泡状或带状扁平/片状的超分子形态结构。结论TEM观察到的土壤形态超分子结构类型与土壤腐殖质不同的化学成分和理化性质有关。这些数据可能有助于更好地了解土壤HAs的整体组成、结构组织以及各种生态和生物地球化学功能。图形抽象
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Transmission electron microscopy indicates supramolecular transformations over time of soil humic acids and their electrophoretic fractions

Transmission electron microscopy indicates supramolecular transformations over time of soil humic acids and their electrophoretic fractions

Background

The influence of various environmental factors on the formation of different morphological forms of soil organic matter has been extensively studied, but there is still uncertainty about how time spent in solution affects the evolution and possible transformation of the morphological structures of soil humic substances.

Methods

The transmission electron microscopy (TEM) with negative contrast staining by uranyl acetate was used to study morphological differences between soil humic acids (HAs) and their individual electrophoretic fractions before and after 150 days of incubation in the same solution under constant environmental conditions.

Results

For the first time, it was found that during 150 days of incubation in constant environmental conditions, soil humic samples can aggregate by two fundamentally different ways, forming vesicle-like or ribbon-flat/sheet-like supramolecular morphological structures.

Conclusions

The types of morphological supramolecular structures observed using TEM were found to be related to different chemical compositions and physical–chemical properties of soil humic substances. These data could be relevant to better understanding the overall makeup of soil HAs, their structural organization, and various ecological and biogeochemical functions.

Graphical Abstract

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来源期刊
Chemical and Biological Technologies in Agriculture
Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
6.80
自引率
3.00%
发文量
83
审稿时长
15 weeks
期刊介绍: Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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