{"title":"马铃薯转录因子StbZIP1通过增强抗氧化能力调控盐碱胁迫反应的分子机制","authors":"Shujuan Jiao, Xiongliang Hu, Yong Wang, Ruyan Zhang, Xingxing Wang, Yuan Lu, Weina Zhang, Yuhui Liu, Shuhao Qin, Yichen Kang","doi":"10.1186/s40538-025-00861-4","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Saline-alkali stress severely impacts global crop productivity, while basic leucine zipper (bZIP) transcription factors (TFs) are known regulators of abiotic stress responses, the specific mechanisms of <i>StbZIP1</i> in potato saline-alkaline tolerance remains unclear.</p><h3>Methods</h3><p>We cloned <i>StbZIP1</i> from tetraploid potato ‘Favorita’, analyzed its sequence characteristics, and generated overexpression lines. <i>StbZIP1</i>-overexpressing (OE) and wild-type (WT) plants were subjected to saline-alkaline stress (NaCl: NaHCO<sub>3</sub> = 1:1) to assess physiological and molecular responses.</p><h3>Results</h3><p>StbZIP1 encodes a 16.61 kDa protein with conserved bZIP domains. Secondary structure prediction revealed that the protein comprises 55.48% α-helix and 44.52% random coil, consistent with the structural characteristics of typical bZIP family features. Physicochemical characterization revealed StbZIP1 was a highly hydrophilic protein (GRAVY index: −0.882) with no transmembrane region, and it harbors 27 predicted phosphorylation sites. Subcellular localization analysis using GFP-tagged StbZIP1 via confocal microscopy confirmed its exclusive nuclear localization, classifying it as a nuclear-targeted transcription factor. Under saline-alkaline stress, WT plants displayed severe wilting and complete desiccation of lower leaves, whereas <i>StbZIP1</i>-OE plants exhibited delayed wilting, no death and retained greener apical leaves. Quantitative analysis revealed that <i>StbZIP1</i>-OE plants showed a 33%–50% increase in chlorophyll content compared to WT (<i>p</i> < 0.01). Notably, <i>StbZIP1</i>-OE plants exhibited a more pronounced increase in Pro content (28% ~ 46%) higher than WT, while their MDA content was significantly reduced compared to WT. Furthermore, the activities of antioxidant enzymes (SOD, POD, and APX) were markedly elevated in <i>StbZIP1</i>-OE plants, showing increases of 81% ~ 100%, 81% ~ 104%, and 20% ~ 43%, respectively, relative to WT. Analysis of stress-related gene expression showed that after 12 d of saline-alkaline stress, the OE plants exhibited significantly increased expression of all six genes (<i>StNCED</i>, <i>StRD29B</i>, <i>StABI5</i>, <i>StP5CS</i>, <i>StSOD</i>, and <i>StCAT</i>) compared with WT (<i>p</i> < 0.05).</p><h3>Conclusions</h3><p><i>StbZIP1</i> positively regulates saline-alkaline tolerance by enhancing antioxidant capacity, providing a reference for the further cultivation of new stress-resistant potatoes.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"12 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00861-4","citationCount":"0","resultStr":"{\"title\":\"Molecular mechanisms of potato (Solanum tuberosum L.) transcription factor StbZIP1 in regulating saline-alkaline stress response through enhanced antioxidant capacity\",\"authors\":\"Shujuan Jiao, Xiongliang Hu, Yong Wang, Ruyan Zhang, Xingxing Wang, Yuan Lu, Weina Zhang, Yuhui Liu, Shuhao Qin, Yichen Kang\",\"doi\":\"10.1186/s40538-025-00861-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Saline-alkali stress severely impacts global crop productivity, while basic leucine zipper (bZIP) transcription factors (TFs) are known regulators of abiotic stress responses, the specific mechanisms of <i>StbZIP1</i> in potato saline-alkaline tolerance remains unclear.</p><h3>Methods</h3><p>We cloned <i>StbZIP1</i> from tetraploid potato ‘Favorita’, analyzed its sequence characteristics, and generated overexpression lines. <i>StbZIP1</i>-overexpressing (OE) and wild-type (WT) plants were subjected to saline-alkaline stress (NaCl: NaHCO<sub>3</sub> = 1:1) to assess physiological and molecular responses.</p><h3>Results</h3><p>StbZIP1 encodes a 16.61 kDa protein with conserved bZIP domains. Secondary structure prediction revealed that the protein comprises 55.48% α-helix and 44.52% random coil, consistent with the structural characteristics of typical bZIP family features. Physicochemical characterization revealed StbZIP1 was a highly hydrophilic protein (GRAVY index: −0.882) with no transmembrane region, and it harbors 27 predicted phosphorylation sites. Subcellular localization analysis using GFP-tagged StbZIP1 via confocal microscopy confirmed its exclusive nuclear localization, classifying it as a nuclear-targeted transcription factor. Under saline-alkaline stress, WT plants displayed severe wilting and complete desiccation of lower leaves, whereas <i>StbZIP1</i>-OE plants exhibited delayed wilting, no death and retained greener apical leaves. Quantitative analysis revealed that <i>StbZIP1</i>-OE plants showed a 33%–50% increase in chlorophyll content compared to WT (<i>p</i> < 0.01). Notably, <i>StbZIP1</i>-OE plants exhibited a more pronounced increase in Pro content (28% ~ 46%) higher than WT, while their MDA content was significantly reduced compared to WT. Furthermore, the activities of antioxidant enzymes (SOD, POD, and APX) were markedly elevated in <i>StbZIP1</i>-OE plants, showing increases of 81% ~ 100%, 81% ~ 104%, and 20% ~ 43%, respectively, relative to WT. Analysis of stress-related gene expression showed that after 12 d of saline-alkaline stress, the OE plants exhibited significantly increased expression of all six genes (<i>StNCED</i>, <i>StRD29B</i>, <i>StABI5</i>, <i>StP5CS</i>, <i>StSOD</i>, and <i>StCAT</i>) compared with WT (<i>p</i> < 0.05).</p><h3>Conclusions</h3><p><i>StbZIP1</i> positively regulates saline-alkaline tolerance by enhancing antioxidant capacity, providing a reference for the further cultivation of new stress-resistant potatoes.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":512,\"journal\":{\"name\":\"Chemical and Biological Technologies in Agriculture\",\"volume\":\"12 1\",\"pages\":\"\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2025-10-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00861-4\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical and Biological Technologies in Agriculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s40538-025-00861-4\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-025-00861-4","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Molecular mechanisms of potato (Solanum tuberosum L.) transcription factor StbZIP1 in regulating saline-alkaline stress response through enhanced antioxidant capacity
Background
Saline-alkali stress severely impacts global crop productivity, while basic leucine zipper (bZIP) transcription factors (TFs) are known regulators of abiotic stress responses, the specific mechanisms of StbZIP1 in potato saline-alkaline tolerance remains unclear.
Methods
We cloned StbZIP1 from tetraploid potato ‘Favorita’, analyzed its sequence characteristics, and generated overexpression lines. StbZIP1-overexpressing (OE) and wild-type (WT) plants were subjected to saline-alkaline stress (NaCl: NaHCO3 = 1:1) to assess physiological and molecular responses.
Results
StbZIP1 encodes a 16.61 kDa protein with conserved bZIP domains. Secondary structure prediction revealed that the protein comprises 55.48% α-helix and 44.52% random coil, consistent with the structural characteristics of typical bZIP family features. Physicochemical characterization revealed StbZIP1 was a highly hydrophilic protein (GRAVY index: −0.882) with no transmembrane region, and it harbors 27 predicted phosphorylation sites. Subcellular localization analysis using GFP-tagged StbZIP1 via confocal microscopy confirmed its exclusive nuclear localization, classifying it as a nuclear-targeted transcription factor. Under saline-alkaline stress, WT plants displayed severe wilting and complete desiccation of lower leaves, whereas StbZIP1-OE plants exhibited delayed wilting, no death and retained greener apical leaves. Quantitative analysis revealed that StbZIP1-OE plants showed a 33%–50% increase in chlorophyll content compared to WT (p < 0.01). Notably, StbZIP1-OE plants exhibited a more pronounced increase in Pro content (28% ~ 46%) higher than WT, while their MDA content was significantly reduced compared to WT. Furthermore, the activities of antioxidant enzymes (SOD, POD, and APX) were markedly elevated in StbZIP1-OE plants, showing increases of 81% ~ 100%, 81% ~ 104%, and 20% ~ 43%, respectively, relative to WT. Analysis of stress-related gene expression showed that after 12 d of saline-alkaline stress, the OE plants exhibited significantly increased expression of all six genes (StNCED, StRD29B, StABI5, StP5CS, StSOD, and StCAT) compared with WT (p < 0.05).
Conclusions
StbZIP1 positively regulates saline-alkaline tolerance by enhancing antioxidant capacity, providing a reference for the further cultivation of new stress-resistant potatoes.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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