方法定量分析小鼠远端结肠和直肠负荷下肌肠丛神经纤维变形

IF 2.3 4区 医学 Q3 ENGINEERING, BIOMEDICAL
Amirhossein Shokrani , Atta Seck , Bin Feng , David M. Pierce
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引用次数: 0

摘要

大肠内脏疼痛是肠易激综合征(IBS)的标志,也是患者寻求胃肠病学治疗的主要原因。值得注意的是,远端结肠和直肠(结直肠)的机械膨胀可靠地引起腹痛,因此了解结直肠感觉神经末梢(神经纤维)的机械转导对于理解和治疗ibs相关的肠痛至关重要。为了促进这种理解,我们的目标是建立新的方法来机械测试,成像和分析大应变变形的神经纤维网络在结直肠的肌肠丛,从而实现定量分析。我们成功地将圆周位移驱动的变形转移到完整的结直肠部分,同时在荧光成像期间保持肌丛聚焦,以捕获变形的神经纤维。我们还建立了一种半自动化的网络形态再现方法和计算小鼠结直肠肌肠丛内变形的单个神经纤维拉伸比的代码。我们的代码允许绘制每根纤维的拉伸比,拉伸比与纤维角度,以及拉伸比与纤维长度。我们的方法不仅有助于分析内脏痛觉背景下结直肠神经纤维网络的变形,而且也适用于分析其他二维纤维网络的平面内变形。我们提供免费的、公开的MATLAB分析代码,包括一个简单测试用例的输入文件,网址为github.uconn.edu/imLab/Fiber-Network_Analyses。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Methods for quantitative analyses of nerve fiber deformation in the myenteric plexus under loading of mouse distal colon and rectum

Methods for quantitative analyses of nerve fiber deformation in the myenteric plexus under loading of mouse distal colon and rectum
Visceral pain in the large bowel is a hallmark of irritable bowel syndrome (IBS) and the primary reason patients seek gastroenterological care. Notably, mechanical distension of the distal colon and rectum (colorectum) reliably evokes abdominal pain and thus understanding mechanotransduction of sensory nerve endings (nerve fibers) in the colorectum is crucial for understanding and treating IBS-related bowel pain. To facilitate such understanding we aimed to establish novel methods to mechanically test, image, and analyze large-strain deformations of networks of nerve fibers in the myenteric plexus of the colorectum, and thus enable quantitative analyses. We successfully delivered circumferential, displacement-driven deformations to intact segments of colorectum while maintaining the myenteric plexus in focus during fluorescent imaging to capture the deforming nerve fibers. We also established a semi-automated method to recapitulate the network morphology and a code to calculate the stretch ratios of individual nerve fibers deforming within the myenteric plexus of mouse colorectum. Our code allows plotting of stretch ratios for each fiber, stretch ratios vs. fiber angles, and stretch ratios vs. fiber lengths. Our methods not only facilitate analyses of deformations of networks of colorectal nerve fibers in the context of visceral nociception but are also applicable to analyzing the in-plane deformation of other two-dimensional fiber networks. We provide free, public access to our analysis code for MATLAB, including input files for a simple test case, at github.uconn.edu/imLab/Fiber-Network_Analyses.
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来源期刊
Medical Engineering & Physics
Medical Engineering & Physics 工程技术-工程:生物医学
CiteScore
4.30
自引率
4.50%
发文量
172
审稿时长
3.0 months
期刊介绍: Medical Engineering & Physics provides a forum for the publication of the latest developments in biomedical engineering, and reflects the essential multidisciplinary nature of the subject. The journal publishes in-depth critical reviews, scientific papers and technical notes. Our focus encompasses the application of the basic principles of physics and engineering to the development of medical devices and technology, with the ultimate aim of producing improvements in the quality of health care.Topics covered include biomechanics, biomaterials, mechanobiology, rehabilitation engineering, biomedical signal processing and medical device development. Medical Engineering & Physics aims to keep both engineers and clinicians abreast of the latest applications of technology to health care.
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