甲氧虫酰肼和氟虫腈通过20E生物合成-免疫双重干扰增强对夜蛾夜蛾的杀虫活性。

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI:10.1021/acs.jafc.5c10854

Zhixian Zhang,Dan Wang,Yajie Ma,Yongpan Shan,Xianpeng Song,Hongyan Hu,Changcai Wu,Yan Ma,Xiangliang Ren

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引用次数: 0

摘要

夜蛾科（鳞翅目，夜蛾科）夜蛾夜蛾对全球农业构成重大威胁，需要通过可持续的替代品，如昆虫生长调节剂（IGRs），包括甲氧虫酰肼（MET）和氟虫腈（LUF）来管理抗性。本研究阐明了IGR暴露下20-羟基蜕皮激素（20E）与免疫信号传导之间的潜在调控机制。亚致死剂量的MET、LUF及其混合物（MML）通过上调SeShd和SeCYP18A1显著下调20E滴度。沉默SeShd降低20E，而敲除SeCYP18A1增加20E；延迟发育和增加对igr的易感性。此外，IGRs激活免疫通路（Toll/IMD），升高SeDif和SePGRP-LB （SePLB）的表达，同时抑制20E。沉默SeDif和SePLB刺激了20E的生物合成，并增加了对IGRs的敏感性。这些发现为20E生物合成与IGRs免疫反应之间复杂的相互作用提供了新的见解。我们的工作增强了对igr分子机制的理解，并为抵抗葡萄球菌的耐药性提供了可行的策略。

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Methoxyfenozide and Lufenuron Enhanced Insecticidal Activity via 20E Biosynthesis-Immunity Dual Disruption in Spodoptera exigua (Lepidoptera, Noctuidae).

Spodoptera exigua (Lepidoptera, Noctuidae) poses significant threats to global agriculture, necessitating the management of resistance through sustainable alternatives such as Insect growth regulators (IGRs), including Methoxyfenozide (MET) and Lufenuron (LUF). This study elucidates the potential regulatory mechanisms between 20-hydroxyecdysone (20E) and immune signaling under IGR exposure. Sublethal doses of MET, LUF, and their mixture (MML) significantly downregulated the 20E titer by upregulating SeShd and SeCYP18A1. Silencing SeShd decreased 20E, whereas knockdown SeCYP18A1 increased 20E; both delayed development and increased susceptibility to IGRs. Furthermore, IGRs activated immune pathways (Toll/IMD), elevating SeDif and SePGRP-LB (SePLB) expression while simultaneously suppressing 20E. Silencing SeDif and SePLB stimulated 20E biosynthesis and heightened susceptibility to IGRs. These findings offer novel insights into the intricate interplay between 20E biosynthesis and the immune response to IGRs. Our work enhances the understanding of the IGRs' molecular mechanism and offers actionable strategies for combating resistance in S. exigua.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.