染色质凝聚体的atp依赖性重塑揭示了不同的中尺度结果

IF 45.8 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2025-10-02 DOI:10.1126/science.adr0018

Camille Moore, Emily Wong, Upneet Kaur, Un Seng Chio, Ziling Zhou, Megan Ostrowski, Ke Wu, Iryna Irkliyenko, Sean Wang, Vijay Ramani, Geeta J. Narlikar

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引用次数: 0

摘要

三磷酸腺苷（ATP）依赖性染色质重塑酶动员核小体，但这种动员如何影响染色质凝聚尚不清楚。我们利用染色质凝聚物和单分子足迹研究了两种主要重塑剂ACF和RSC的作用。我们发现这两种重塑剂都抑制了浓缩染色质的形成。然而，重塑剂对预形成的染色质凝聚物有明显的影响。ACF在不降低染色质的情况下给核小体空间，解释了ACF如何在转录抑制的基因组区域维持核小体的组织。相比之下，RSC催化atp依赖性的染色质去浓缩。RSC还驱动整个染色质凝聚体的微米级运动。这些额外的RSC活性可能有助于其在转录中的核心作用。重塑蛋白的生物学重要性可能反映了它们对核小体动员的影响以及在中尺度上对染色质动力学的相应影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

ATP-dependent remodeling of chromatin condensates reveals distinct mesoscale outcomes

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ATP-dependent remodeling of chromatin condensates reveals distinct mesoscale outcomes

Adenosine triphosphate (ATP)–dependent chromatin remodeling enzymes mobilize nucleosomes, but how such mobilization affects chromatin condensation is unclear. We investigate effects of two major remodelers, ACF and RSC, using chromatin condensates and single-molecule footprinting. We find that both remodelers inhibit the formation of condensed chromatin. However, the remodelers have distinct effects on preformed chromatin condensates. ACF spaces nucleosomes without decondensing the chromatin, explaining how ACF maintains nucleosome organization in transcriptionally repressed genomic regions. By contrast, RSC catalyzes ATP-dependent decondensation of chromatin. RSC also drives micron-scale movements of entire chromatin condensates. These additional activities of RSC may contribute to its central role in transcription. The biological importance of remodelers may thus reflect both their effects on nucleosome mobilization and the corresponding consequences on chromatin dynamics at the mesoscale.

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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