硒- gpx4轴减轻花生四烯酸诱导的精子损伤。

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-30 DOI:10.1021/acs.jafc.5c05677

Huihui Tian,Jiaying Chen,Shijie Fan,Pengyu Cao,Luxi Lin,Hao Zhao,Qingyu Zhao,Junmin Zhang,Chaohua Tang

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引用次数: 0

摘要

硒（Se）和谷胱甘肽过氧化物酶4 （GPX4）对男性生殖、调节精子发生和抵抗过氧化损伤至关重要。花生四烯酸（AA）与男性生育能力密切相关，但过量摄入会对精子质量产生不利影响。然而，Se-GPX4轴如何改善aa诱导的睾丸和精子损伤仍不清楚。本研究建立aa致精子损伤模型。结合硒补充和睾丸特异性GPX4基因敲除，我们测量了睾丸损伤和精子质量表型，并通过组学探讨了潜在的机制。结果表明，硒通过调节GPX4表达、缓解氧化应激、细胞凋亡、性激素紊乱和炎症，改善aa诱导的睾丸/精子损伤。相反，GPX4基因敲除加重了这种损伤。氧脂组学揭示了睾丸多不饱和脂肪酸（PUFA）代谢物的变化，而转录组学指出了关键途径。总之，Se-GPX4轴可能通过调节PUFA代谢物、激活谷胱甘肽代谢、减轻aa诱导的睾丸氧化应激、细胞凋亡和炎症以及修复类固醇激素的生物合成来改善精子损伤。

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Selenium-GPX4 Axis Relieves Arachidonic Acid-Induced Sperm Damage.

Selenium (Se) and glutathione peroxidase 4 (GPX4) are essential for male reproduction, regulating spermatogenesis, and resisting peroxidative damage. Arachidonic acid (AA) is closely related to male fertility, but excessive intake adversely affects sperm quality. However, how the Se-GPX4 axis ameliorates AA-induced testicular and sperm injury remains unclear. In this study, an AA-induced sperm injury model was established. Combined with Se supplementation and testicular-specific GPX4 knockout, we measured testicular injury and sperm quality phenotypes and explored the potential mechanism via omics. Results showed that Se supplementation improved AA-induced testicular/sperm damage by regulating GPX4 expression, relieving oxidative stress, apoptosis, sex hormone disorders, and inflammation. Conversely, the GPX4 knockout exacerbated this damage. Oxylipidomics revealed changes in testicular polyunsaturated fatty acid (PUFA) metabolites, while transcriptomics pointed to key pathways. Overall, the Se-GPX4 axis may ameliorate sperm damage by regulating PUFA metabolites, activating glutathione metabolism, alleviating AA-induced testicular oxidative stress, apoptosis and inflammation, and repairing steroid hormone biosynthesis.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.