基于31P NMR和4D-DIA定量蛋白质组学表征高压脉冲电场处理对乳脂球膜蛋白和磷脂的影响

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-09-28 DOI:10.1016/j.foodchem.2025.146515

Xiankang Fan , Jia Tang , Yufei Chu , Yi Wang , Pengjie Wang , Hao Zhang , Jie Luo

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引用次数: 0

摘要

乳脂球膜（MFGM）促进新生儿大脑发育，尽管其提取和恢复一直具有挑战性。本文研究了高压脉冲电场（PEF）处理对MFGM蛋白和磷脂的影响。结果表明，PEF处理显著降低了乳脂球的粒径，提高了zeta电位绝对值，改善了微观结构的稳定性。与巴氏灭菌法相比,PEF 40倍(35 kv /厘米)治疗显著增加蛋白质(XO / XDH BTN, PAS6/7 ADRP)和磷脂(磷脂酰胆碱,磷脂酰肌醇,磷脂酰丝氨酸和磷脂酰乙醇胺)含量(0.94 ±0.01  g / 100 毫升和273.40 ±4.60  毫克/公斤),经济复苏(67.35 ±0.13   %和96.47±1.60  %)MFGM。这种改善可能是由于非热PEF处理在灭菌过程中改变了MFGM的渗透性，从而减少了酪蛋白和乳清蛋白的吸附。蛋白质组学和31P NMR进一步支持了这些发现。该研究为MFGM的提取和应用提供了重要的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Characterization of the effects of high voltage pulsed electric field treatment on milk fat globule membrane proteins and phospholipid based on 31P NMR and 4D-DIA quantitative proteomics

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Characterization of the effects of high voltage pulsed electric field treatment on milk fat globule membrane proteins and phospholipid based on 31P NMR and 4D-DIA quantitative proteomics

The milk fat globule membrane (MFGM) promotes brain development in newborns, although its extraction and recovery have been challenging. This study investigated the effects of high-voltage pulsed electric field (PEF) treatment on MFGM proteins and phospholipids. The results demonstrated that PEF treatment significantly reduced the particle size of milk fat globules, increased the absolute zeta potential value, and improved microstructure stability. Compared with pasteurization, PEF (35 kv/cm for 40 times) treatment significantly increased protein (XO/XDH, BTN, PAS6/7, and ADRP) and phospholipids (phosphatidylcholine, phosphatidylinositol phosphatidylserine, and phosphatidylethanolamine) content (0.94 ± 0.01 g/100 mL and 273.40 ± 4.60 mg/kg) and the recovery (67.35 ± 0.13 % and 96.47 ± 1.60 %) in MFGM. This improvement may result from the non-thermal PEF treatment altering MFGM permeability during sterilization, thereby reducing the adsorption of casein and whey proteins. Proteomic and ³¹P NMR further supported these findings. This study provides critical insights for MFGM extraction and application.

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.