初乳饲喂量为出生体重的6%、8%、10%或12%对荷斯坦犊牛IgG吸收效率、胃排空和摄食后行为的影响

IF 4.4 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
G Frederick, M Wieland, A Singh, R Ewing, M A Steele, H Somula, S Mann
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引用次数: 0

摘要

我们的目的是评估初次饲喂不同量初乳对IgG吸收、胃排空和犊牛行为的影响。雌性荷斯坦犊牛(n = 88)采用随机区组设计,分别饲喂相当于出生体重(BBW) 6%、8%、10%或12%的初乳。8组动物(n = 32头小牛)也接受了胃排空标志物对乙酰氨基酚(Ac),剂量为150 mg/kg代谢体重(BBW0.75)。初乳在出生后2小时内通过食管饲管给予。分别于饲喂前(0 h)和饲喂后24 h采集血样。对于接受Ac治疗的犊牛,在饲喂后2、4、6、8、10和48 h采集血浆样本。采用径向免疫扩散法测定初乳和24 h血清IgG浓度。24 h血清IgG浓度计算表观吸收效率(AEA)。采用酶促比色法测定血浆Ac浓度,并建立8 h时胃中Ac残留比例(Acs)模型。用自定义线图分析饲喂后头2小时小牛行为的音频和视频记录。采用混合模型或Fisher精确检验进行数据分析。结果报告为LSM, 95% CI,除非另有说明。犊牛平均胸围(范围)为40 (31.8 ~ 49.1)kg,混合初乳白锐度中值(范围)为24.2% (21.2% ~ 29.6%),IgG浓度为86.7 g/L (73.2 ~ 121.1 g/L)。6%、8%、10%和12%组犊牛初乳体积中值(L)分别为2.3(2.0 ~ 2.8)、3.1(2.5 ~ 3.9)、4.0(3.5 ~ 4.5)和4.8(4.2 ~ 5.6)。24 h血清IgG浓度(g/L)以6%组最低(28.8[25.8-31.8]),8%组最高(37.4(34.4-40.4),10%和12%组最高(分别为41.1[38.1-44.1]和43.4[40.4-46.4])。各组间AEA(%)随摄食率的升高而逐渐下降,以6%和8%组最高(分别为47.8[44.9-50.7]和46.2[43.3-49.0]),10%组降至41.0(38.1-43.9),12%组最低,为36.3(33.5-39.2)。8 h时,6%、8%、10%、12%组的Acs(%)分别为原剂量的50.4(43.8 ~ 57.0)、55.3(49.2 ~ 61.5)、57.9(51.8 ~ 64.0)、65.5(59.2 ~ 71.8)。在踢腿(一种类似绞痛的行为)方面,发现了组间的行为差异,仅在10% (n = 21次踢腿)和12% (n = 40次踢腿)组中观察到这种行为。在出生后2小时内饲喂相当于乳脂重8%或10%的初乳,可以在IgG吸收、胃排空和犊牛行为方面做出妥协。更大的体积(12% BBW)导致血清IgG浓度的边际改善,AEA和胃排空减少,并与绞痛样行为增加相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of feeding colostrum volume at 6%, 8%, 10%, or 12% of birth body weight on efficiency of IgG absorption, gastric emptying, and postfeeding behavior in Holstein calves.

Our objective was to evaluate how feeding colostrum at different volumes at the first feeding affects IgG absorption, gastric emptying, and calf behavior. Female Holstein calves (n = 88) were enrolled in a randomized block design to receive a single colostrum volume corresponding to 6%, 8%, 10%, or 12% of birth body weight (BBW). A subset of animals in 8 blocks (n = 32 calves) also received a gastric emptying marker acetaminophen (Ac) at a dose of 150 mg/kg metabolic body weight (BBW0.75). Colostrum was administered within 2 h of birth via esophageal tube feeder. Blood samples were collected before (0 h) and 24 h after feeding. For the calves receiving Ac, plasma samples were also collected at 2, 4, 6, 8, 10, and 48 h after feeding. Colostrum and 24-h serum IgG concentrations were measured using radial immunodiffusion. Apparent efficiency of absorption (AEA) was calculated from 24-h serum IgG concentrations. Plasma Ac concentration was determined using an enzymatic colorimetric assay, and the proportion of Ac remaining in the stomach (Acs) at 8 h was modeled. Audio and video recordings of calf behavior during the first 2 h after feeding were analyzed with a custom ethogram. Data analysis was performed using mixed models or Fisher's exact tests as appropriate. Results are reported as LSM with 95% CI unless otherwise specified. The mean (range) BBW of calves was 40 (31.8-49.1) kg. Pooled colostrum had a median (range) Brix value of 24.2% (21.2%-29.6%) and IgG concentrations of 86.7 g/L (73.2-121.1 g/L). Calves in the 6%, 8%, 10%, and 12% groups were fed median (range) colostrum volumes (L) of 2.3 (2.0-2.8), 3.1 (2.5-3.9), 4.0 (3.5-4.5), and 4.8 (4.2-5.6), respectively. The 24-h serum IgG concentrations (g/L) were lowest in the 6% group (28.8 [25.8-31.8]), increased to 37.4 (34.4-40.4) in the 8% group, and were highest in the 10% and 12% groups (41.1 [38.1-44.1] and 43.4 [40.4-46.4], respectively). Group differences were observed for AEA (%), which gradually declined with an increasing feeding rate and was highest in the 6% and 8% groups (47.8 [44.9-50.7] and 46.2 [43.3-49.0], respectively), declined to 41.0 (38.1-43.9) in the 10% group, and was lowest at 36.3 (33.5-39.2) in the 12% group. At 8 h, Acs (%) was 50.4 (43.8-57.0), 55.3 (49.2-61.5), 57.9 (51.8-64.0), and 65.5 (59.2-71.8) of the original dose in the 6%, 8%, 10%, and 12% groups, respectively. Group differences in behavior were identified for kicking, a colic-like behavior, which was observed only in the 10% (n = 21 kicks total) and 12% (n = 40 kicks total) groups. Feeding a single colostrum meal equivalent to 8% or 10% of BBW within 2 h after birth offers a compromise in terms of IgG absorption, gastric emptying, and calf behavior. A larger volume (12% BBW) resulted in marginal improvement in serum IgG concentration, reduced AEA and gastric emptying, and was associated with increased colic-like behaviors.

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来源期刊
Journal of Dairy Science
Journal of Dairy Science 农林科学-奶制品与动物科学
CiteScore
7.90
自引率
17.10%
发文量
784
审稿时长
4.2 months
期刊介绍: The official journal of the American Dairy Science Association®, Journal of Dairy Science® (JDS) is the leading peer-reviewed general dairy research journal in the world. JDS readers represent education, industry, and government agencies in more than 70 countries with interests in biochemistry, breeding, economics, engineering, environment, food science, genetics, microbiology, nutrition, pathology, physiology, processing, public health, quality assurance, and sanitation.
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