围产期缺磷对奶牛红细胞膜磷脂组成的影响。

IF 4.4 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Klaus Eder, Sarah M Grundmann, Jos F Brouwers, Lianne M van den Brink, Imke Cohrs, Walter Grünberg
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引用次数: 0

摘要

先前的研究表明,奶牛在过渡时期磷供应不足可导致产后血红蛋白尿,这与贫血的发展有关,可能是红细胞渗透性脆弱性增加的结果。这种情况的确切原因尚不清楚。本研究的假设是,奶牛在过渡阶段的磷消耗导致红细胞膜磷脂成分的变化,这可能有助于增加渗透脆弱性。为了验证这一假设,本试验选取了18头健康的多胎妊娠奶牛,从预期产犊前4周至产后4周,分别饲喂磷含量充足的对照饲粮(干期和哺乳期DM中磷含量分别为0.28%和0.44%,对照组)和磷含量降低的饲粮(干期和哺乳期DM中磷含量分别为0.15%和0.20%,缺磷组)。在预计产犊日期前2周以及产后1周和4周采集血液样本。分离红细胞膜,提取脂质,采用液相色谱-质谱法对单个磷脂种类进行定量分析。磷脂类含量(鞘磷脂、磷脂酰丝氨酸、磷脂酰乙醇胺[PE]、磷脂酰肌醇、磷脂酰胆碱[PC]、溶酶-PC和溶酶-PE)不受处理的影响。同样,单个磷脂的平均碳原子链长和平均双键数也不受处理的影响。个别磷脂种类的百分比变化很小。采样时间对磷脂组成和单个磷脂种类的比例只有极小的影响。综上所述,适度和短暂的饲粮缺磷不会改变红细胞膜磷脂组成。这表明缺磷引起的围产期血红蛋白尿不是由红细胞膜脂组成的改变引起的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of phosphorus deprivation during the periparturient period on the phospholipid composition of erythrocyte membranes in dairy cows.

Previous studies have shown that insufficient phosphorus supply in dairy cows during the transition period can lead to postparturient hemoglobinuria, which is associated with the development of anemia and is presumably a consequence of increased osmotic fragility of erythrocytes. The exact causes of this condition remain unknown. The present study investigated the hypothesis that phosphorus depletion in dairy cows during the transition phase leads to changes in the phospholipid composition of the erythrocyte membrane, which may contribute to increased osmotic fragility. To test this hypothesis, an experiment with 18 healthy, multiparous, pregnant cows was conducted, in which cows received either a control diet with adequate phosphorus content (0.28% and 0.44% phosphorus in DM during the dry period and lactation, respectively, control group) or a diet with reduced phosphorus content (0.15% and 0.20% phosphorus in DM during the dry period and lactation, respectively, phosphorus depletion group) from 4 wk before expected calving until 4 wk postpartum. Blood samples were collected 2 wk before the expected calving date, as well as 1 and 4 wk postpartum. Erythrocyte membranes were isolated, and lipids were extracted for quantitative analysis of individual phospholipid species using liquid chromatography-MS. Phospholipid class contents (sphingomyelin, phosphatidylserine, phosphatidylethanolamine [PE], phosphatidylinositol, phosphatidylcholine [PC], lyso-PC, and lyso-PE) were not affected by the treatment. Similarly, the mean carbon atom chain length and the mean number of double bonds in individual phospholipids were not affected by treatment. There were very few changes of the percentages of individual phospholipid species. The time of sampling had only minimal effect on phospholipid composition and the proportions of individual phospholipid species. In conclusion, these results indicate that moderate and transient dietary phosphorus deprivation does not alter the phospholipid composition of the erythrocyte membrane. This suggests that phosphorus deprivation-induced periparturient hemoglobinuria is not caused by alterations in erythrocyte membrane lipid composition.

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来源期刊
Journal of Dairy Science
Journal of Dairy Science 农林科学-奶制品与动物科学
CiteScore
7.90
自引率
17.10%
发文量
784
审稿时长
4.2 months
期刊介绍: The official journal of the American Dairy Science Association®, Journal of Dairy Science® (JDS) is the leading peer-reviewed general dairy research journal in the world. JDS readers represent education, industry, and government agencies in more than 70 countries with interests in biochemistry, breeding, economics, engineering, environment, food science, genetics, microbiology, nutrition, pathology, physiology, processing, public health, quality assurance, and sanitation.
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