希腊药用植物中常见的多酚类化合物对登革热和黄热病病毒的抗氧化和抗病毒活性的评价。

IF 6.6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Eirini Kyriakopoulou, Aliki Tsakni, Evangelos Korakidis, George Mpekoulis, Katerina I Kalliampakou, Monika Polanska, Jan F M Van Impe, Efstathia Tsakali, Dimitra Houhoula, Niki Vassilaki
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引用次数: 0

摘要

多酚类化合物普遍存在于希腊药用植物中,具有抗病毒和抗氧化的特性,具有潜在的治疗用途。本研究旨在评估9种选定的多酚对登革热病毒(DENV)和黄热病病毒(YFV)生命周期的抗病毒活性,以及它们通过DPPH法和ABTS法测定的抗氧化能力,以及它们抑制过氧自由基诱导的DNA链断裂的能力。山奈酚和咖啡酸对DENV基因组复制的抑制作用最强,而香豆酸更有效地阻断病毒进入。值得注意的是,山奈酚的抗denv作用最强,特别是在病毒释放感染性水平上,其EC50最低(3.55 μΜ),选择性指数最高(SI = 25.45)。相比之下,没有一种化合物对YFV基因组复制显示出显著的抗病毒活性。同时,咖啡酸和山奈酚具有最高的自由基清除活性(DPPH和ABTS测定),突出了它们的双重性质。此外,DNA断裂抑制实验证实了所有化合物具有较强的抗氧化潜力,其中咖啡酸和山奈酚的抑制率最高,分别为98.98%和97.34%。这些发现强调了特定多酚,特别是山奈酚和咖啡酸,作为针对DENV和氧化应激相关损伤的抗病毒和抗氧化剂的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Evaluation of Polyphenolic Compounds Common in Greek Medicinal Plants for Their Antioxidant Effects and Antiviral Activity Against Dengue and Yellow Fever Viruses.

Evaluation of Polyphenolic Compounds Common in Greek Medicinal Plants for Their Antioxidant Effects and Antiviral Activity Against Dengue and Yellow Fever Viruses.

Evaluation of Polyphenolic Compounds Common in Greek Medicinal Plants for Their Antioxidant Effects and Antiviral Activity Against Dengue and Yellow Fever Viruses.

Evaluation of Polyphenolic Compounds Common in Greek Medicinal Plants for Their Antioxidant Effects and Antiviral Activity Against Dengue and Yellow Fever Viruses.

Polyphenolic compounds, commonly found in Greek medicinal plants, exhibit promising antiviral and antioxidant properties, making them potential candidates for therapeutic purposes. This study aims to evaluate the antiviral activity of nine selected polyphenols against Dengue virus (DENV) and Yellow Fever virus (YFV) life cycles, alongside their antioxidant capacity determined by the DPPH method and the ABTS assay, and their ability to inhibit DNA strand scission induced by peroxyl radicals. Kaempferol and caffeic acid demonstrated the most potent inhibitory effects on DENV genome replication, while coumaric acid blocked viral entry more effectively. Notably, among the nine compounds, kaempferol exhibited the strongest anti-DENV effect, especially at the level of virus-released infectivity, showing the lowest EC50 (3.55 μΜ) and the highest selectivity index (SI = 25.45). In contrast, none of the compounds showed significant antiviral activity against YFV genome replication. Concomitantly, caffeic acid and kaempferol had the highest radical scavenging activity (DPPH and ABTS assays), highlighting their dual properties. Moreover, DNA scission inhibition assays confirmed the strong antioxidant potential of all tested compounds, with caffeic acid and kaempferol achieving the highest inhibition rate of 98.98% and 97.34% respectively. These findings underscore the potential of specific polyphenols, particularly kaempferol and caffeic acid, as antiviral and antioxidant agents targeting DENV and oxidative stress-related damage.

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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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