蒲公英(Taraxacum mongolicum)隐藏的宝藏:具有增强生物活性的酶解多糖,用于ROS防御和发育保护

IF 5.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Yue Zheng, Juan Du, Yuan Wang, Wenwen Wang, Xiaoping An, Jingwei Qi
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引用次数: 0

摘要

蒲公英(Taraxacum mongolicum)作为天然添加剂广泛应用于食品、制药和化妆品行业。本研究利用果胶酶对蒲公英进行酶解,以提高其生物活性。结果表明,酶处理破坏了细胞壁基质,使原料从黄绿色变成深棕色,并释放出结合的多糖。经水解提取的EDP多糖含量较高(180.72 mg/g),水提率提高(33.22%),分子量降低。体外实验结果表明,其对EDP-84.06% DPPH和59%羟基自由基的清除能力较好,并具有较强的还原能力。此外,在aaph诱导的斑马鱼模型中,EDP更有效地支持心率和正常发育,同时显著减少活性氧(ROS)积累、脂质过氧化和细胞死亡。总的来说,这些发现强调了酶水解作为一种提高蒲公英生物活性特性的策略的潜力,为其在功能食品和天然抗氧化剂中的应用提供了有价值的见解。图形抽象
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unlocking dandelion (Taraxacum mongolicum)’s hidden treasure: enzyme-hydrolyzed polysaccharides with enhanced bioactivity for ROS defense and developmental protection

Dandelion (Taraxacum mongolicum) is widely used as a natural additive in food, pharmaceutical, and cosmetic industries. In this study, we applied pectinase-mediated enzymatic hydrolysis to dandelion to enhance its bioactivity. The results showcased that enzyme treatment disrupted the cell‐wall matrix, turning the raw material from yellowish-green to dark brown and releasing bound polysaccharides. The hydrolyzed extract polysaccharide (EDP) exhibited higher polysaccharide content (180.72 mg/g), an improved water extraction rate (33.22%), and a reduced molecular weight. In vitro assays revealed superior antioxidant performance in EDP—84.06% DPPH and 59% hydroxyl-radical scavenging—along with stronger reducing power. Furthermore, in an AAPH-induced zebrafish model, EDP more effectively supported heart rate and normal development while notably reducing reactive oxygen species (ROS) accumulation, lipid peroxidation, and cell death. Collectively, these findings highlight the potential of enzymatic hydrolysis as a strategy to boost the bioactive properties of dandelion, offering valuable insights for its use in functional foods and natural antioxidants.

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来源期刊
Chemical and Biological Technologies in Agriculture
Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
6.80
自引率
3.00%
发文量
83
审稿时长
15 weeks
期刊介绍: Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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