饲粮中添加牛至精油可改善肉牛肠道屏障功能,调节盲肠菌群和代谢物

IF 5.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Pengjia He, Yu Lei, Rui Zhang, Jinping Shi, Qiang Cheng, Yannan Ma, Tao Ran, Zhaomin Lei
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引用次数: 0

摘要

肉牛后肠的健康对其生长和整体健康起着至关重要的作用。牛至精油(OEO)由于其多种有益特性,如抗菌和抗炎作用,作为抗生素的潜在替代品而受到关注。然而,其对肉牛盲肠健康的具体影响及其潜在机制尚未完全了解。本研究旨在研究添加OEO对肉牛基础免疫状态、肠道屏障完整性相关基因表达、盲肠微生物群及其代谢物的影响,从而更好地了解其潜在机制。结果选取27头初始体重为270.47±16.26 kg的阉牛,随机分为3组,每组9个重复,试验期390 d。试验饲喂基础饲粮(CON)、基础饲粮中添加130 mg/d OEO (LOE)和基础饲粮中添加260 mg/d OEO (HOE)。结果表明,饲粮中添加HOE显著提高了生长性能(平均日增重和末重)(P < 0.05)。与CON组相比,LOE组和HOE组均降低了白细胞介素(IL)-1β、IL-2和肿瘤坏死因子α (TNF-α)水平(P < 0.05),而HOE组仅降低了IL-6,升高了IL-4 (P < 0.05)。此外,添加HOE可上调肠紧密连接蛋白(ZO-1和Claudin-1)基因表达(P < 0.05)。HOE组盲肠有益菌(如芽孢杆菌、乳杆菌和乳球菌)的相对丰度显著增加,P < 0.05)。此外,饲粮中添加羟基乙酸可提高盲肠短链脂肪酸中丁酸盐的浓度(P < 0.05)。结论补充hoe可调节盲肠屏障稳态、微生物群及其衍生代谢物,改善基础免疫状态,最终有利于肉牛的健康和生产性能。图形抽象
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dietary oregano essential oil supplementation improves intestinal barrier function and modulates the cecal microbiota and metabolites of beef cattle

Background

The health of the hindgut in beef cattle plays a crucial role in their growth and overall health. Oregano essential oil (OEO) has gained attention as a potential alternative to antibiotics due to its various beneficial properties such as antibacterial and anti-inflammatory effects. However, its specific impacts on cecal health in beef cattle and its underlying mechanisms are yet to be fully understood. This study aimed to investigate the effects of OEO supplementation on basal immune status, intestinal barrier integrity-related gene expression, microbiota, and their metabolites in the cecum of beef cattle, thus to better understand its potential mechanism.

Results

Twenty-seven steers (initial body weigh 270.47 ± 16.26 kg) were randomly assigned to three groups with nine replicates each in a 390-day trial. Steers were fed with basal diet (CON), basal diet supplemented with 130 mg/d OEO (LOE), and basal diet with 260 mg/d OEO (HOE). Results showed that dietary HOE supplementation significantly increased the growth performance (average daily gain and final body weight) (P < 0.05). Compared to CON group, both LOE and HOE groups showed reduced levels of interleukin (IL)-1β, IL-2, and tumor necrosis factor α (TNF-α) (P < 0.05), while only HOE decreased IL-6 and increased IL-4 (P < 0.05). Moreover, HOE supplementation upregulated the intestinal tight junction proteins (ZO-1 and Claudin-1) gene expression (P < 0.05). The HOE group exhibited a significant increase in the relative abundances of cecal beneficial bacteria (e.g., Bacillus, Lactobacillus, and Lactococcus, P < 0.05). Furthermore, HOE supplementation increased the concentrations of butyrate in cecal short-chain fatty acids (P < 0.05).

Conclusion

HOE supplementation has the potential to regulate cecal barrier homeostasis, the microbiota and its derived metabolites, improve basal immune status, and ultimately benefit the health and performance of steers.

Graphical Abstract

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来源期刊
Chemical and Biological Technologies in Agriculture
Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
6.80
自引率
3.00%
发文量
83
审稿时长
15 weeks
期刊介绍: Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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