Pengjia He, Yu Lei, Rui Zhang, Jinping Shi, Qiang Cheng, Yannan Ma, Tao Ran, Zhaomin Lei
{"title":"饲粮中添加牛至精油可改善肉牛肠道屏障功能,调节盲肠菌群和代谢物","authors":"Pengjia He, Yu Lei, Rui Zhang, Jinping Shi, Qiang Cheng, Yannan Ma, Tao Ran, Zhaomin Lei","doi":"10.1186/s40538-025-00858-z","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>The health of the hindgut in beef cattle plays a crucial role in their growth and overall health. Oregano essential oil (OEO) has gained attention as a potential alternative to antibiotics due to its various beneficial properties such as antibacterial and anti-inflammatory effects. However, its specific impacts on cecal health in beef cattle and its underlying mechanisms are yet to be fully understood. This study aimed to investigate the effects of OEO supplementation on basal immune status, intestinal barrier integrity-related gene expression, microbiota, and their metabolites in the cecum of beef cattle, thus to better understand its potential mechanism.</p><h3>Results</h3><p>Twenty-seven steers (initial body weigh 270.47 ± 16.26 kg) were randomly assigned to three groups with nine replicates each in a 390-day trial. Steers were fed with basal diet (CON), basal diet supplemented with 130 mg/d OEO (LOE), and basal diet with 260 mg/d OEO (HOE). Results showed that dietary HOE supplementation significantly increased the growth performance (average daily gain and final body weight) (<i>P</i> < 0.05). Compared to CON group, both LOE and HOE groups showed reduced levels of interleukin (IL)-1β, IL-2, and tumor necrosis factor α (TNF-α) (<i>P</i> < <i>0.05</i>), while only HOE decreased IL-6 and increased IL-4 (<i>P</i> < 0.05). Moreover, HOE supplementation upregulated the intestinal tight junction proteins (ZO-1 and Claudin-1) gene expression <i>(P</i> < <i>0.05)</i>. The HOE group exhibited a significant increase in the relative abundances of cecal beneficial bacteria (e.g., <i>Bacillus, Lactobacillus, and Lactococcus, P</i> < <i>0.05</i>). Furthermore, HOE supplementation increased the concentrations of butyrate in cecal short-chain fatty acids (<i>P</i> < <i>0.05</i>).</p><h3>Conclusion</h3><p>HOE supplementation has the potential to regulate cecal barrier homeostasis, the microbiota and its derived metabolites, improve basal immune status, and ultimately benefit the health and performance of steers.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"12 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00858-z","citationCount":"0","resultStr":"{\"title\":\"Dietary oregano essential oil supplementation improves intestinal barrier function and modulates the cecal microbiota and metabolites of beef cattle\",\"authors\":\"Pengjia He, Yu Lei, Rui Zhang, Jinping Shi, Qiang Cheng, Yannan Ma, Tao Ran, Zhaomin Lei\",\"doi\":\"10.1186/s40538-025-00858-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>The health of the hindgut in beef cattle plays a crucial role in their growth and overall health. Oregano essential oil (OEO) has gained attention as a potential alternative to antibiotics due to its various beneficial properties such as antibacterial and anti-inflammatory effects. However, its specific impacts on cecal health in beef cattle and its underlying mechanisms are yet to be fully understood. This study aimed to investigate the effects of OEO supplementation on basal immune status, intestinal barrier integrity-related gene expression, microbiota, and their metabolites in the cecum of beef cattle, thus to better understand its potential mechanism.</p><h3>Results</h3><p>Twenty-seven steers (initial body weigh 270.47 ± 16.26 kg) were randomly assigned to three groups with nine replicates each in a 390-day trial. Steers were fed with basal diet (CON), basal diet supplemented with 130 mg/d OEO (LOE), and basal diet with 260 mg/d OEO (HOE). Results showed that dietary HOE supplementation significantly increased the growth performance (average daily gain and final body weight) (<i>P</i> < 0.05). Compared to CON group, both LOE and HOE groups showed reduced levels of interleukin (IL)-1β, IL-2, and tumor necrosis factor α (TNF-α) (<i>P</i> < <i>0.05</i>), while only HOE decreased IL-6 and increased IL-4 (<i>P</i> < 0.05). Moreover, HOE supplementation upregulated the intestinal tight junction proteins (ZO-1 and Claudin-1) gene expression <i>(P</i> < <i>0.05)</i>. The HOE group exhibited a significant increase in the relative abundances of cecal beneficial bacteria (e.g., <i>Bacillus, Lactobacillus, and Lactococcus, P</i> < <i>0.05</i>). 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Dietary oregano essential oil supplementation improves intestinal barrier function and modulates the cecal microbiota and metabolites of beef cattle
Background
The health of the hindgut in beef cattle plays a crucial role in their growth and overall health. Oregano essential oil (OEO) has gained attention as a potential alternative to antibiotics due to its various beneficial properties such as antibacterial and anti-inflammatory effects. However, its specific impacts on cecal health in beef cattle and its underlying mechanisms are yet to be fully understood. This study aimed to investigate the effects of OEO supplementation on basal immune status, intestinal barrier integrity-related gene expression, microbiota, and their metabolites in the cecum of beef cattle, thus to better understand its potential mechanism.
Results
Twenty-seven steers (initial body weigh 270.47 ± 16.26 kg) were randomly assigned to three groups with nine replicates each in a 390-day trial. Steers were fed with basal diet (CON), basal diet supplemented with 130 mg/d OEO (LOE), and basal diet with 260 mg/d OEO (HOE). Results showed that dietary HOE supplementation significantly increased the growth performance (average daily gain and final body weight) (P < 0.05). Compared to CON group, both LOE and HOE groups showed reduced levels of interleukin (IL)-1β, IL-2, and tumor necrosis factor α (TNF-α) (P < 0.05), while only HOE decreased IL-6 and increased IL-4 (P < 0.05). Moreover, HOE supplementation upregulated the intestinal tight junction proteins (ZO-1 and Claudin-1) gene expression (P < 0.05). The HOE group exhibited a significant increase in the relative abundances of cecal beneficial bacteria (e.g., Bacillus, Lactobacillus, and Lactococcus, P < 0.05). Furthermore, HOE supplementation increased the concentrations of butyrate in cecal short-chain fatty acids (P < 0.05).
Conclusion
HOE supplementation has the potential to regulate cecal barrier homeostasis, the microbiota and its derived metabolites, improve basal immune status, and ultimately benefit the health and performance of steers.
期刊介绍:
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.