{"title":"超声微波辅助提取菠萝蜜果皮多糖的工艺优化及对肠道健康的影响","authors":"Kit-Leong Cheong, Keying Liu, Yun-Feng Li, Chen Huang, Saiyi Zhong, Karsoon Tan, Malairaj Sathuvan","doi":"10.1186/s40538-025-00790-2","DOIUrl":null,"url":null,"abstract":"<div><p>Improper disposal of jackfruit peel waste poses environmental risks, but its polysaccharides (PJP) offer potential health benefits. This study aimed to optimize the ultrasound-microwave-assisted extraction conditions for PJP and evaluate their structural characteristics as well as intestinal health activity. Using Box–Behnken design, the optimal extraction yield of 24.6% was achieved under the following conditions: solid-to-liquid ratio of 48.7 mL/g, extraction time of 29.8 min, and extraction temperature of 81.9 °C. Structural analysis revealed that PJP primarily consists of glucose units with a backbone linked by → 4)-β-D-Glc<i>p</i>-(1 →. In DSS-induced colitis mice, PJP increased the abundance of intestinal microorganisms (<i>Bacteroides</i> and <i>Lactobacillus</i>), enhanced the production of short-chain fatty acids (SCFAs), and reduced inflammation (TNF-α, IL-1β), while enhancing the levels of tight junction proteins (claudin-1, occludin, ZO-1). This study provides a methodological framework for polysaccharide research and provides preliminary evidence for the industrial production and practical application of PJP. </p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"12 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00790-2","citationCount":"0","resultStr":"{\"title\":\"Optimizing ultrasound-microwave-assisted extraction of jackfruit peel polysaccharides and evaluating their impact on intestinal health in vivo\",\"authors\":\"Kit-Leong Cheong, Keying Liu, Yun-Feng Li, Chen Huang, Saiyi Zhong, Karsoon Tan, Malairaj Sathuvan\",\"doi\":\"10.1186/s40538-025-00790-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Improper disposal of jackfruit peel waste poses environmental risks, but its polysaccharides (PJP) offer potential health benefits. This study aimed to optimize the ultrasound-microwave-assisted extraction conditions for PJP and evaluate their structural characteristics as well as intestinal health activity. Using Box–Behnken design, the optimal extraction yield of 24.6% was achieved under the following conditions: solid-to-liquid ratio of 48.7 mL/g, extraction time of 29.8 min, and extraction temperature of 81.9 °C. Structural analysis revealed that PJP primarily consists of glucose units with a backbone linked by → 4)-β-D-Glc<i>p</i>-(1 →. In DSS-induced colitis mice, PJP increased the abundance of intestinal microorganisms (<i>Bacteroides</i> and <i>Lactobacillus</i>), enhanced the production of short-chain fatty acids (SCFAs), and reduced inflammation (TNF-α, IL-1β), while enhancing the levels of tight junction proteins (claudin-1, occludin, ZO-1). This study provides a methodological framework for polysaccharide research and provides preliminary evidence for the industrial production and practical application of PJP. </p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":512,\"journal\":{\"name\":\"Chemical and Biological Technologies in Agriculture\",\"volume\":\"12 1\",\"pages\":\"\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2025-07-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00790-2\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical and Biological Technologies in Agriculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s40538-025-00790-2\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-025-00790-2","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
不当处理菠萝蜜果皮废弃物会带来环境风险，但其多糖（PJP）具有潜在的健康益处。本研究旨在优化超声微波辅助提取PJP的条件，并评价其结构特征和肠道健康活性。采用Box-Behnken设计，在料液比为48.7 mL/g、提取时间为29.8 min、提取温度为81.9℃的条件下，提取率为24.6%。结构分析表明PJP主要由葡萄糖单元组成，其主链由→4)-β- d - glcp -(1→连接。在dss诱导的结肠炎小鼠中，PJP增加了肠道微生物（拟杆菌和乳杆菌）的丰度，增加了短链脂肪酸（SCFAs）的产生，减少了炎症（TNF-α, IL-1β），同时提高了紧密连接蛋白（claudin-1, occludin, ZO-1）的水平。本研究为多糖研究提供了一个方法学框架，为PJP的工业化生产和实际应用提供了初步依据。图形抽象
Optimizing ultrasound-microwave-assisted extraction of jackfruit peel polysaccharides and evaluating their impact on intestinal health in vivo
Improper disposal of jackfruit peel waste poses environmental risks, but its polysaccharides (PJP) offer potential health benefits. This study aimed to optimize the ultrasound-microwave-assisted extraction conditions for PJP and evaluate their structural characteristics as well as intestinal health activity. Using Box–Behnken design, the optimal extraction yield of 24.6% was achieved under the following conditions: solid-to-liquid ratio of 48.7 mL/g, extraction time of 29.8 min, and extraction temperature of 81.9 °C. Structural analysis revealed that PJP primarily consists of glucose units with a backbone linked by → 4)-β-D-Glcp-(1 →. In DSS-induced colitis mice, PJP increased the abundance of intestinal microorganisms (Bacteroides and Lactobacillus), enhanced the production of short-chain fatty acids (SCFAs), and reduced inflammation (TNF-α, IL-1β), while enhancing the levels of tight junction proteins (claudin-1, occludin, ZO-1). This study provides a methodological framework for polysaccharide research and provides preliminary evidence for the industrial production and practical application of PJP.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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