Pia Eckhof , Nélida Nina , Sarah Bauer , Thomas A Kufer , Jan Frank , Felipe Jiménez-Aspee
{"title":"从豆(Phaseolus vulgaris L.)地方种中提取的生物可及化合物可激活瞬时转染的HepG2细胞中的PPARγ","authors":"Pia Eckhof , Nélida Nina , Sarah Bauer , Thomas A Kufer , Jan Frank , Felipe Jiménez-Aspee","doi":"10.1016/j.nutres.2025.09.001","DOIUrl":null,"url":null,"abstract":"<div><div>Common beans (<em>Phaseolus vulgaris</em> L.) are a nutrient-dense staple food associated with a reduced risk of noncommunicable chronic diseases. One proposed mechanism involves the modulation of nuclear receptors, particularly peroxisome proliferator-activated receptors (PPARs), which are key regulators of lipid and carbohydrate metabolism. In this study, we evaluated the effects of <em>in vitro</em> digested flours, secondary metabolite-enriched extracts (SMEEs), their fractions, and selected representative phytochemicals from 14 landraces and 2 commercial <em>P. vulgaris</em> samples to directly activate PPARs and other nuclear receptors using a luciferase reporter assay in transiently transfected HepG2 cells. We hypothesized that bioaccessible compounds from the digested landraces would directly activate PPARγ in HepG2 cells. Digested flours from landraces such as Magnum and Peumo significantly activated both PPARγ1 and PPARγ2, reaching values up to 1.54-fold higher than the controls (<em>P</em> < .05). Corresponding SMEEs showed stronger activities, with up to 2.32-fold activation compared to the controls (<em>P</em> < .05). In contrast, none of the polarity-based fractions or the major identified phytochemicals (kaempferol-3-O-glycoside, sojasaponin Ba and Bb) showed significant effects. These findings demonstrate that digested <em>P. vulgaris</em> matrices contain bioaccessible compounds capable of directly activating PPARγ in HepG2 cells, likely through synergistic interactions or unidentified constituents. This mechanistic evidence supports the functional relevance of traditional bean landraces in precision nutrition strategies targeting metabolic health.</div></div>","PeriodicalId":19245,"journal":{"name":"Nutrition Research","volume":"142 ","pages":"Pages 33-45"},"PeriodicalIF":3.1000,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bioaccessible compounds from bean (Phaseolus vulgaris L.) landraces activate PPARγ in transiently transfected HepG2 cells\",\"authors\":\"Pia Eckhof , Nélida Nina , Sarah Bauer , Thomas A Kufer , Jan Frank , Felipe Jiménez-Aspee\",\"doi\":\"10.1016/j.nutres.2025.09.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Common beans (<em>Phaseolus vulgaris</em> L.) are a nutrient-dense staple food associated with a reduced risk of noncommunicable chronic diseases. One proposed mechanism involves the modulation of nuclear receptors, particularly peroxisome proliferator-activated receptors (PPARs), which are key regulators of lipid and carbohydrate metabolism. In this study, we evaluated the effects of <em>in vitro</em> digested flours, secondary metabolite-enriched extracts (SMEEs), their fractions, and selected representative phytochemicals from 14 landraces and 2 commercial <em>P. vulgaris</em> samples to directly activate PPARs and other nuclear receptors using a luciferase reporter assay in transiently transfected HepG2 cells. We hypothesized that bioaccessible compounds from the digested landraces would directly activate PPARγ in HepG2 cells. Digested flours from landraces such as Magnum and Peumo significantly activated both PPARγ1 and PPARγ2, reaching values up to 1.54-fold higher than the controls (<em>P</em> < .05). Corresponding SMEEs showed stronger activities, with up to 2.32-fold activation compared to the controls (<em>P</em> < .05). In contrast, none of the polarity-based fractions or the major identified phytochemicals (kaempferol-3-O-glycoside, sojasaponin Ba and Bb) showed significant effects. These findings demonstrate that digested <em>P. vulgaris</em> matrices contain bioaccessible compounds capable of directly activating PPARγ in HepG2 cells, likely through synergistic interactions or unidentified constituents. This mechanistic evidence supports the functional relevance of traditional bean landraces in precision nutrition strategies targeting metabolic health.</div></div>\",\"PeriodicalId\":19245,\"journal\":{\"name\":\"Nutrition Research\",\"volume\":\"142 \",\"pages\":\"Pages 33-45\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2025-09-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nutrition Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0271531725001101\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nutrition Research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0271531725001101","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
Bioaccessible compounds from bean (Phaseolus vulgaris L.) landraces activate PPARγ in transiently transfected HepG2 cells
Common beans (Phaseolus vulgaris L.) are a nutrient-dense staple food associated with a reduced risk of noncommunicable chronic diseases. One proposed mechanism involves the modulation of nuclear receptors, particularly peroxisome proliferator-activated receptors (PPARs), which are key regulators of lipid and carbohydrate metabolism. In this study, we evaluated the effects of in vitro digested flours, secondary metabolite-enriched extracts (SMEEs), their fractions, and selected representative phytochemicals from 14 landraces and 2 commercial P. vulgaris samples to directly activate PPARs and other nuclear receptors using a luciferase reporter assay in transiently transfected HepG2 cells. We hypothesized that bioaccessible compounds from the digested landraces would directly activate PPARγ in HepG2 cells. Digested flours from landraces such as Magnum and Peumo significantly activated both PPARγ1 and PPARγ2, reaching values up to 1.54-fold higher than the controls (P < .05). Corresponding SMEEs showed stronger activities, with up to 2.32-fold activation compared to the controls (P < .05). In contrast, none of the polarity-based fractions or the major identified phytochemicals (kaempferol-3-O-glycoside, sojasaponin Ba and Bb) showed significant effects. These findings demonstrate that digested P. vulgaris matrices contain bioaccessible compounds capable of directly activating PPARγ in HepG2 cells, likely through synergistic interactions or unidentified constituents. This mechanistic evidence supports the functional relevance of traditional bean landraces in precision nutrition strategies targeting metabolic health.
期刊介绍:
Nutrition Research publishes original research articles, communications, and reviews on basic and applied nutrition. The mission of Nutrition Research is to serve as the journal for global communication of nutrition and life sciences research on diet and health. The field of nutrition sciences includes, but is not limited to, the study of nutrients during growth, reproduction, aging, health, and disease.
Articles covering basic and applied research on all aspects of nutrition sciences are encouraged, including: nutritional biochemistry and metabolism; metabolomics, nutrient gene interactions; nutrient requirements for health; nutrition and disease; digestion and absorption; nutritional anthropology; epidemiology; the influence of socioeconomic and cultural factors on nutrition of the individual and the community; the impact of nutrient intake on disease response and behavior; the consequences of nutritional deficiency on growth and development, endocrine and nervous systems, and immunity; nutrition and gut microbiota; food intolerance and allergy; nutrient drug interactions; nutrition and aging; nutrition and cancer; obesity; diabetes; and intervention programs.