奶牛初乳和乳中干细胞的纵向变化。

IF 1.2 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Laurence Finot, Marion Boutinaud, Hélène Quesnel, Eric Chanat
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引用次数: 0

摘要

本研究旨在鉴定和量化奶牛泌乳初期初乳和乳中的各种干细胞类型。5秒胎次荷斯坦奶牛从产犊到产后第7天进行监测。产犊后立即收集乳腺分泌物,然后在第0天每3 h收集一次,直到12 h,并在第1、2、4和7天的早晨挤奶时收集一次。从乳腺分泌物中制备细胞,用流式细胞术分析相关细胞标志物。在产犊时和6 h内收集的初乳中观察到最高的总细胞和活细胞浓度,在第0 d后收集的样品中这些浓度大幅下降。然后,总细胞群和活细胞群的浓度继续缓慢下降,直到第7天,动力学曲线达到基线平台。流式细胞术显示,鉴定乳腺上皮干细胞的CD49fposCD24pos群体在产犊时约占活细胞的0.9%,12 h后约占活细胞的0.1%,因此,乳腺上皮干细胞浓度在第一次初乳时达到最高水平。相比之下,在前两次挤奶期间,间充质干细胞样细胞(定义为CD34negCD105posCD90posCD29pos细胞群)的百分比大致恒定(≈0.3%),在第一天主要下降至接近0的基础水平。关于造血干细胞样细胞,定义为CD45negCD34posCD117posCD90pos细胞群,它们仅在产犊时收集的初乳中观察到。因此,这里研究的所有类型的干细胞都只在产犊后最初几个小时的初乳中大量存在,在这段时间里,小牛的肠道具有渗透性,可能允许这些细胞在新生小牛的组织中转移和整合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Longitudinal changes of stem-like cells in colostrum and milk of dairy cows.

This study aimed to identify and quantify the various stem-like cell types in dairy cows' colostrum and milk at the onset of lactation. Five second parity Holstein cows were monitored from calving until the seventh-day postpartum. Mammary secretions were collected immediately after calving, then every 3 h until 12 h during day (d) 0, and during morning milking on d 1, d 2, d 4 and d 7. Cells were prepared from mammary secretions and analysed by flow cytometry using relevant cellular markers. The highest total and viable cell concentrations were observed in colostrum collected at calving and up to 6 h, with these concentrations decreasing substantially in samples collected later at d 0. Then, the concentrations of both total and viable cell populations continued to slowly decrease until d 7, the kinetic curves reaching a baseline plateau. Flow cytometry showed that the CD49fposCD24pos population, which identifies mammary epithelial stem cells, represented about 0.9% of viable cells at calving and about 0.1% 12 h later, the mammary epithelial stem cell concentration therefore being at its highest level in the very first colostrum. In contrast, the percentage of mesenchymal stem-like cells, defined as the population of CD34negCD105posCD90posCD29pos cells, was roughly constant (≈0.3%) during the first two milkings and decreased mainly during the first day to a basal level close to 0. Concerning haematopoietic stem-like cells, defined as the CD45negCD34posCD117posCD90pos cell population, they were only observed in the colostrum collected at calving. All the types of stem cells studied here were therefore only present in substantial quantities in the colostrum of the very first hours after calving, a period during which the calf's intestine is permeable, possibly allowing the transfer and integration of these cells in the tissues of the newborn calf.

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来源期刊
Journal of Dairy Research
Journal of Dairy Research 农林科学-奶制品与动物科学
CiteScore
3.80
自引率
4.80%
发文量
117
审稿时长
12-24 weeks
期刊介绍: The Journal of Dairy Research is an international Journal of high-standing that publishes original scientific research on all aspects of the biology, wellbeing and technology of lactating animals and the foods they produce. The Journal’s ability to cover the entire dairy foods chain is a major strength. Cross-disciplinary research is particularly welcomed, as is comparative lactation research in different dairy and non-dairy species and research dealing with consumer health aspects of dairy products. Journal of Dairy Research: an international Journal of the lactation sciences.
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