代谢组学分析揭示了鞘脂途径在小麦斑点病抗性中的作用

IF 2.2 4区 生物学 Q2 PLANT SCIENCES
Sagar Yadav, Saborni Maiti, Swapnil Mundhe, Narendra Kadoo
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引用次数: 0

摘要

小麦斑疹病是由土壤和空气传播的真菌病原菌引起的,是小麦生产的主要威胁。该病在小麦种植区,特别是在南亚、南美洲、非洲和澳大利亚,正达到流行病的程度。在印度,超过2500万公顷的小麦种植区受到这种疾病的威胁。对代谢物的系统研究可以深入了解这种疾病的分子基础。本研究评价了接种白僵菌对小麦品种Chirya3(抗斑病)和DDK1025(感斑病)的影响。我们使用液相色谱和高分辨率质谱(LC-HRMS)对接种病原体和模拟接种的植物进行了时间过程的非靶向代谢物分析。进行多变量分析以构建全面的统计工作流程,从而确定“代谢组学表型”。利用正交投影潜结构判别分析(OPLS-DA)模型揭示了抗病和敏感品种对病原菌接种反应的显著代谢物。在感染进展过程中,共有699种代谢物表现出显著的变化。B. sorokiniana接种的Chirya3的代谢产物如鞘脂、半胱氨酸、苯丙氨酸、莽草酸盐等含量较高。研究表明鞘脂通路在促进小麦木质化和抗病性增强的抗性机制中起关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Metabolomic profiling unravels the role of sphingolipid pathways in spot blotch resistance in wheat

Spot blotch, caused by the soil- and air-borne fungal pathogen Bipolaris sorokiniana, is a major threat to wheat production. The disease is reaching epidemic proportions in wheat-growing areas, particularly in South Asia, South America, Africa, and Australia. In India, over 25 million hectares of wheat-growing area is threatened by this disease. A systematic study of metabolites can provide insights into the molecular basis of this disease. In the present study, we evaluated the impact of B. sorokiniana inoculation on two wheat varieties, Chirya3 (resistant to spot blotch) and DDK1025 (susceptible to spot blotch). We performed time-course non-targeted metabolite profiling of the pathogen-inoculated and mock-inoculated plants using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS). Multivariate analysis was performed to construct a comprehensive statistical workflow, which led to the defined “metabolomic phenotypes”. Modeling by Orthogonal Projection to Latent Structures-Discriminant Analysis (OPLS-DA) revealed significant metabolites in responses of the resistant and susceptible varieties to pathogen inoculation. A total of 699 metabolites displayed significant variations during the progression of infection. B. sorokiniana-inoculated Chirya3 exhibited high levels of some metabolites, such as sphingolipids, cysteine, phenylalanine, shikimates, etc. The study revealed that sphingolipid pathways are critical in resistance mechanisms contributing to enhanced lignification and disease resistance in wheat.

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来源期刊
Acta Physiologiae Plantarum
Acta Physiologiae Plantarum 生物-植物科学
CiteScore
5.10
自引率
3.80%
发文量
125
审稿时长
3.1 months
期刊介绍: Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry. The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.
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