Nannan Wang, Man Zhang, Liangjie Niu, Monica Scali, Weili Huang, Wei Wang
{"title":"玉米根系对短期渗透胁迫的生理生化和蛋白质组学分析","authors":"Nannan Wang, Man Zhang, Liangjie Niu, Monica Scali, Weili Huang, Wei Wang","doi":"10.1007/s11738-025-03811-7","DOIUrl":null,"url":null,"abstract":"<div><p>Drought is a major abiotic stress that adversely impacts on crop productivity and global food security. Proteomics greatly facilitates the study of stress response mechanism in crop plants by identifying new stress-responsive proteins. Currently, most proteomic studies on osmotic stress response in cereal crops, such as maize, have mainly focused on medium to long-term scales, but few studies investigated the early osmotic stress response. In this study, maize roots were subjected to 30 min 10% polyethylene glycol (PEG)-6000 induced osmotic stress, the physiological, biochemical and proteomic changes were analyzed. The results showed that short-term osmotic stress led to the significant increase of reactive oxygen species (ROS, especially O<sub>2</sub><sup>−</sup>) level, the content of thiobarbituric acid reactive substances (TBARS) and proline, and the activity of catalase (CAT) and superoxide dismutase (SOD) in maize root tips. Proteomics analysis identified a set of 20 stress-responsive proteins, which were mainly involved in antioxidative stress and energy metabolism (e.g., ATP synthase), and most of them were more abundant under osmotic stress. Obviously, the antioxidant system and energy metabolism pathway play critical roles in the early root response to short-term osmotic stress. Our work provides insights into the mechanisms underlying the early response of plants to osmotic stress. </p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 6","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Physiological, biochemical and proteomic analyses of root response of maize to short-term osmotic stress\",\"authors\":\"Nannan Wang, Man Zhang, Liangjie Niu, Monica Scali, Weili Huang, Wei Wang\",\"doi\":\"10.1007/s11738-025-03811-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Drought is a major abiotic stress that adversely impacts on crop productivity and global food security. Proteomics greatly facilitates the study of stress response mechanism in crop plants by identifying new stress-responsive proteins. Currently, most proteomic studies on osmotic stress response in cereal crops, such as maize, have mainly focused on medium to long-term scales, but few studies investigated the early osmotic stress response. In this study, maize roots were subjected to 30 min 10% polyethylene glycol (PEG)-6000 induced osmotic stress, the physiological, biochemical and proteomic changes were analyzed. The results showed that short-term osmotic stress led to the significant increase of reactive oxygen species (ROS, especially O<sub>2</sub><sup>−</sup>) level, the content of thiobarbituric acid reactive substances (TBARS) and proline, and the activity of catalase (CAT) and superoxide dismutase (SOD) in maize root tips. Proteomics analysis identified a set of 20 stress-responsive proteins, which were mainly involved in antioxidative stress and energy metabolism (e.g., ATP synthase), and most of them were more abundant under osmotic stress. Obviously, the antioxidant system and energy metabolism pathway play critical roles in the early root response to short-term osmotic stress. Our work provides insights into the mechanisms underlying the early response of plants to osmotic stress. </p></div>\",\"PeriodicalId\":6973,\"journal\":{\"name\":\"Acta Physiologiae Plantarum\",\"volume\":\"47 6\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-06-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Physiologiae Plantarum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11738-025-03811-7\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Physiologiae Plantarum","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s11738-025-03811-7","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Physiological, biochemical and proteomic analyses of root response of maize to short-term osmotic stress
Drought is a major abiotic stress that adversely impacts on crop productivity and global food security. Proteomics greatly facilitates the study of stress response mechanism in crop plants by identifying new stress-responsive proteins. Currently, most proteomic studies on osmotic stress response in cereal crops, such as maize, have mainly focused on medium to long-term scales, but few studies investigated the early osmotic stress response. In this study, maize roots were subjected to 30 min 10% polyethylene glycol (PEG)-6000 induced osmotic stress, the physiological, biochemical and proteomic changes were analyzed. The results showed that short-term osmotic stress led to the significant increase of reactive oxygen species (ROS, especially O2−) level, the content of thiobarbituric acid reactive substances (TBARS) and proline, and the activity of catalase (CAT) and superoxide dismutase (SOD) in maize root tips. Proteomics analysis identified a set of 20 stress-responsive proteins, which were mainly involved in antioxidative stress and energy metabolism (e.g., ATP synthase), and most of them were more abundant under osmotic stress. Obviously, the antioxidant system and energy metabolism pathway play critical roles in the early root response to short-term osmotic stress. Our work provides insights into the mechanisms underlying the early response of plants to osmotic stress.
期刊介绍:
Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry.
The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.