玉米根系对短期渗透胁迫的生理生化和蛋白质组学分析

IF 2.2 4区 生物学 Q2 PLANT SCIENCES
Nannan Wang, Man Zhang, Liangjie Niu, Monica Scali, Weili Huang, Wei Wang
{"title":"玉米根系对短期渗透胁迫的生理生化和蛋白质组学分析","authors":"Nannan Wang,&nbsp;Man Zhang,&nbsp;Liangjie Niu,&nbsp;Monica Scali,&nbsp;Weili Huang,&nbsp;Wei Wang","doi":"10.1007/s11738-025-03811-7","DOIUrl":null,"url":null,"abstract":"<div><p>Drought is a major abiotic stress that adversely impacts on crop productivity and global food security. Proteomics greatly facilitates the study of stress response mechanism in crop plants by identifying new stress-responsive proteins. Currently, most proteomic studies on osmotic stress response in cereal crops, such as maize, have mainly focused on medium to long-term scales, but few studies investigated the early osmotic stress response. In this study, maize roots were subjected to 30 min 10% polyethylene glycol (PEG)-6000 induced osmotic stress, the physiological, biochemical and proteomic changes were analyzed. The results showed that short-term osmotic stress led to the significant increase of reactive oxygen species (ROS, especially O<sub>2</sub><sup>−</sup>) level, the content of thiobarbituric acid reactive substances (TBARS) and proline, and the activity of catalase (CAT) and superoxide dismutase (SOD) in maize root tips. Proteomics analysis identified a set of 20 stress-responsive proteins, which were mainly involved in antioxidative stress and energy metabolism (e.g., ATP synthase), and most of them were more abundant under osmotic stress. Obviously, the antioxidant system and energy metabolism pathway play critical roles in the early root response to short-term osmotic stress. Our work provides insights into the mechanisms underlying the early response of plants to osmotic stress. </p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 6","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Physiological, biochemical and proteomic analyses of root response of maize to short-term osmotic stress\",\"authors\":\"Nannan Wang,&nbsp;Man Zhang,&nbsp;Liangjie Niu,&nbsp;Monica Scali,&nbsp;Weili Huang,&nbsp;Wei Wang\",\"doi\":\"10.1007/s11738-025-03811-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Drought is a major abiotic stress that adversely impacts on crop productivity and global food security. Proteomics greatly facilitates the study of stress response mechanism in crop plants by identifying new stress-responsive proteins. Currently, most proteomic studies on osmotic stress response in cereal crops, such as maize, have mainly focused on medium to long-term scales, but few studies investigated the early osmotic stress response. In this study, maize roots were subjected to 30 min 10% polyethylene glycol (PEG)-6000 induced osmotic stress, the physiological, biochemical and proteomic changes were analyzed. The results showed that short-term osmotic stress led to the significant increase of reactive oxygen species (ROS, especially O<sub>2</sub><sup>−</sup>) level, the content of thiobarbituric acid reactive substances (TBARS) and proline, and the activity of catalase (CAT) and superoxide dismutase (SOD) in maize root tips. Proteomics analysis identified a set of 20 stress-responsive proteins, which were mainly involved in antioxidative stress and energy metabolism (e.g., ATP synthase), and most of them were more abundant under osmotic stress. Obviously, the antioxidant system and energy metabolism pathway play critical roles in the early root response to short-term osmotic stress. Our work provides insights into the mechanisms underlying the early response of plants to osmotic stress. </p></div>\",\"PeriodicalId\":6973,\"journal\":{\"name\":\"Acta Physiologiae Plantarum\",\"volume\":\"47 6\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-06-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Physiologiae Plantarum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11738-025-03811-7\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Physiologiae Plantarum","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s11738-025-03811-7","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

干旱是一种主要的非生物胁迫,对作物生产力和全球粮食安全产生不利影响。蛋白质组学通过鉴定新的胁迫响应蛋白,极大地促进了作物胁迫响应机制的研究。目前,对玉米等谷类作物渗透胁迫响应的蛋白质组学研究大多集中在中长期尺度上,而对渗透胁迫早期响应的研究较少。本研究对玉米根系进行10%聚乙二醇(PEG)-6000诱导的渗透胁迫处理30 min,分析其生理生化和蛋白质组学变化。结果表明,短期渗透胁迫导致玉米根尖活性氧(ROS)含量、硫代巴比妥酸活性物质(TBARS)和脯氨酸含量、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性显著升高,O2−含量尤其显著;蛋白质组学分析鉴定出20个应激反应蛋白,这些蛋白主要参与抗氧化应激和能量代谢(如ATP合成酶),其中大多数在渗透胁迫下含量更高。显然,抗氧化系统和能量代谢途径在根系对短期渗透胁迫的早期响应中起关键作用。我们的工作为了解植物对渗透胁迫的早期反应机制提供了见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Physiological, biochemical and proteomic analyses of root response of maize to short-term osmotic stress

Drought is a major abiotic stress that adversely impacts on crop productivity and global food security. Proteomics greatly facilitates the study of stress response mechanism in crop plants by identifying new stress-responsive proteins. Currently, most proteomic studies on osmotic stress response in cereal crops, such as maize, have mainly focused on medium to long-term scales, but few studies investigated the early osmotic stress response. In this study, maize roots were subjected to 30 min 10% polyethylene glycol (PEG)-6000 induced osmotic stress, the physiological, biochemical and proteomic changes were analyzed. The results showed that short-term osmotic stress led to the significant increase of reactive oxygen species (ROS, especially O2) level, the content of thiobarbituric acid reactive substances (TBARS) and proline, and the activity of catalase (CAT) and superoxide dismutase (SOD) in maize root tips. Proteomics analysis identified a set of 20 stress-responsive proteins, which were mainly involved in antioxidative stress and energy metabolism (e.g., ATP synthase), and most of them were more abundant under osmotic stress. Obviously, the antioxidant system and energy metabolism pathway play critical roles in the early root response to short-term osmotic stress. Our work provides insights into the mechanisms underlying the early response of plants to osmotic stress.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Acta Physiologiae Plantarum
Acta Physiologiae Plantarum 生物-植物科学
CiteScore
5.10
自引率
3.80%
发文量
125
审稿时长
3.1 months
期刊介绍: Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry. The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信