Serratia sp. H6促进拟南芥生长需要代谢应激传感器snf1相关蛋白激酶1 (SnRK1)

IF 2.2 4区 生物学 Q2 PLANT SCIENCES
Diana Vázquez-Lemus, Esmeralda Guadalupe Rodríguez-Cuevas, Alma Alejandra Hernández-Esquivel, Elda Castro-Mercado, Homero Reyes-de la Cruz, Jesús Campos-García, José López-Bucio, Ernesto García-Pineda
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引用次数: 0

摘要

SNF1(蔗糖非发酵1)相关蛋白激酶(SnRK1)是出芽酵母SNF1和哺乳动物amp活化蛋白激酶(AMPK)的植物同源物。这些保守的蛋白质就像代谢传感器一样,在能量水平下降时被激活。本研究采用分子和药理学方法,分析了SnRK1在低能量条件下拟南芥与植物生长促进根细菌(PGPR) Serratia sp. H6相互作用中的作用。在植物生长培养基中缺乏蔗糖的情况下,Serratia sp. H6植物刺激并促进了拟南芥突变体kin10的生长,通过侧根数、根长和鲜重的变化来评估,这损害了SnRK1的催化活性。在相同生长条件下,哺乳动物AMPK抑制剂dorsomorphin (DM)在5 μM浓度下显著抑制植物生长和根分生组织细胞分裂,抑制根细菌对植物的刺激作用。相比之下,在Serratia sp. H6存在的情况下,添加25或50 μM的白藜芦醇(resveratrol, RSV)(一种哺乳动物AMPK的激活剂),在不添加蔗糖的情况下,促进了根分生组织的细胞分裂,侧根的形成,并对拟沙菌有植物刺激作用。RSV无法在kin10和snrk1.3突变株中恢复植物刺激。这些药理学研究表明,SnRK1活性是根细菌在低能量水平下促进拟南芥生长所必需的。我们的研究结果表明,SnRK1在代谢应激下由Serratia sp. H6促进的拟南芥生长中起关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The growth promotion of Arabidopsis thaliana by Serratia sp. H6 requires the metabolic stress sensor SNF1-related protein kinase 1 (SnRK1)

The growth promotion of Arabidopsis thaliana by Serratia sp. H6 requires the metabolic stress sensor SNF1-related protein kinase 1 (SnRK1)

The SNF1 (sucrose non-fermenting1)-related protein kinase1 (SnRK1) is the plant ortholog of the budding yeast SNF1 and mammalian AMP-activated protein kinase (AMPK). These conserved proteins function as metabolic sensors activated in response to decreasing energy levels. Here, we analyzed the role of SnRK1 in the interaction of Arabidopsis thaliana with the plant growth-promoting rhizobacterium (PGPR) Serratia sp. H6, under low-energy conditions, using molecular and pharmacological approaches. In the absence of sucrose in the plant growth medium, Serratia sp. H6 phytostimulated and promoted the growth of the A. thaliana mutant kin10, which has impaired SnRK1 catalytic activity, as assessed by changes in lateral root number, root length, and fresh weight. Under the same growth conditions, the mammalian AMPK inhibitor dorsomorphin (DM) at 5 μM notably decreased plant growth and cell division in the root meristem, inhibiting the phytostimulation by the rhizobacterium. In contrast, the addition of resveratrol (RSV) at 25 or 50 μM, an activator of mammalian AMPK, in the presence of Serratia sp. H6 and without sucrose, improved cell division in the root meristem, the formation of lateral roots, and the phytostimulatory effects on A. thaliana. RSV was unable to restore phytostimulation in kin10 and snrk1.3 mutant plants. These pharmacological studies suggest that SnRK1 activity is required for the growth promotion of A. thaliana by the rhizobacterium under low-energy levels. Our findings show that SnRK1 plays a key role in the growth of A. thaliana promoted by Serratia sp. H6, under metabolic stress.

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来源期刊
Acta Physiologiae Plantarum
Acta Physiologiae Plantarum 生物-植物科学
CiteScore
5.10
自引率
3.80%
发文量
125
审稿时长
3.1 months
期刊介绍: Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry. The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.
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