强化比色-荧光碳点纳米复合材料双读数横向流动免疫分析法检测霉素

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-09-26 DOI:10.1016/j.foodchem.2025.146398

Hongfang Li, Wenqing Xu, Yina Zhu, Hongxing Wei, Xiaoyue Liu, Lin Li, Guoliang Li

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引用次数: 0

摘要

传统的以金纳米颗粒为探针的横向流动免疫分析法（LFIA）在检测食品中的霉素时存在灵敏度不足和准确性差的问题。开发具有荧光和比色信号的双读出LFIA可以显著提高其性能。将荧光碳点包裹在SiO2纳米球中，得到量子产率为81.26 %的SiO2/CDs纳米复合材料。这种封装提高了碳点的信号强度，防止了碳点的荧光猝灭。用聚多巴胺（PDA）修饰SiO2/CDs表面，实现了高效的抗体偶联。PDA的强光吸收能力也赋予了纳米复合材料良好的比色信号。与传统的LFIA相比，SiO2/CDs@PDA-based LFIA在比色和荧光模式下对氯霉素的检测分别提高了5倍和71倍。鸡体内氨霉素的定量检出限分别为1.03 ~ 1.54 ng/g和0.1 ~ 0.78 ng/g。提出的LFIA为食品安全监测提供了一种快速、准确、可靠的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Dual-readout lateral flow immunoassay for detection of amphenicols using intensified colorimetric–fluorescent carbon dots nanocomposite

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Dual-readout lateral flow immunoassay for detection of amphenicols using intensified colorimetric–fluorescent carbon dots nanocomposite

Conventional lateral flow immunoassays (LFIA) employing gold nanoparticles as probes suffer from insufficient sensitivity and poor accuracy in detecting amphenicols in food. Developing a dual-readout LFIA with fluorescence and colorimetric signals can significantly enhance its performance. Herein, fluorescent carbon dots were encapsulated into SiO₂ nanospheres to obtain the SiO₂/CDs nanocomposites with a quantum yield of 81.26 %. The encapsulation enhances the signal intensity and prevents fluorescence quenching of carbon dots. Surface modification of SiO₂/CDs with polydopamine (PDA) enables efficient antibody conjugation. The strong light absorption capacity of PDA also endowed the nanocomposites with good colorimetric signals. The SiO₂/CDs@PDA-based LFIA showed 5-fold and 71-fold improvements in chloramphenicol detection using colorimetric and fluorescent modes, compared to conventional LFIA. The quantitative detection limits were 1.03–1.54 ng/g and 0.1–0.78 ng/g in colorimetric and fluorescent mode for amphenicols in chicken. The proposed LFIA offers a rapid, accurate, and reliable method for food safety monitoring.

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.