Programmable Bimetallic MOF Nanozyme Composite with Microenvironment-Adaptive Cascade Catalysis for Wearable Glucose Biosensing and Accelerated Diabetic Wound Healing

The dual challenge of continuous glucose monitoring and treating chronic, infected wounds in diabetes demands innovative materials with integrated diagnostic and therapeutic capabilities. Herein, engineered through biomimetic coencapsulation of glucose oxidase (GOx) and a bimetallic metal–organic framework (MOF), MOF-919 within ZIF-8, this study reports a programmable nanozyme composite─GMZ. MOF-919’s spatially resolved Fe/Cu active sites enable microenvironment-adaptive catalysis: Fe nodes drive peroxidase (POD)-like ^•OH generation for antibacterial action, while Cu centers switch to antioxidant superoxide dismutase (SOD)/catalase (CAT)-like functions at physiological pH, with density functional theory (DFT) calculations confirming Fe-mediated electronic optimization reduces ROS-scavenging energy barriers. The ZIF-8 matrix enhances GOx stability and confinement-accelerated cascade kinetics, enabling a wearable sweat glucose patch and a nanofiber dressing that eradicates bacteria via glucose oxidation producing H₂O₂ and resolves oxidative stress to remodel chronic wounds. This work presents a singular nanozyme platform that seamlessly bridges noninvasive biosensing and intelligent tissue regeneration, offering a powerful and unified strategy for the comprehensive management of diabetes.