Justine Gordon, , , Seyed Danial Mousavi, , , Jing Li, , , Nina Filippone, , , Lindsey Walter, , , Han-Wen Cheng, , , Zakiya Skeete, , , Hallie Feldman, , , Sydney Hakimi, , , Kaylee Cappuccio, , , Shan Wang, , , Melissa Hader, , , Guojun Shang, , , James Turner, , , Andrew Cameron, , , Susan Bane, , , Mark Poliks, , , Susan Lu, , , Tony T. Yuan, , and , Chuan-Jian Zhong*,
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The nanoprobes are labeled with Raman reporter molecules for diagnostic detection and quantitative analysis of the protein or virus concentrations in the samples. Key to the detection is the capture of the targeted biomolecules by specific antibody-conjugated gold nanoparticles in a high-surface-area fibrous paper substrate, which facilitates the formation of “hot spots” for SERS signal amplification. The relationships between the SERS intensity and sample concentration were determined to assess diagnostic sensitivity and limits of detection. The results were also validated using the polymerase chain reaction (PCR) cycle threshold (CT) value vs the concentration of SARS-CoV-2. The nanoprobe-based and fibrous paper-assisted approach demonstrated a low-cost and portable platform technology for enhanced SERS detection of SARS-CoV-2, showing promise for expansion to the detection of various viruses.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 39","pages":"21303–21313"},"PeriodicalIF":6.7000,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Plasmonic Nanoprobe-Enabled SERS Detection of SARS-CoV-2 Proteins and Virus Samples on Wax-Printed Paper Substrates\",\"authors\":\"Justine Gordon, , , Seyed Danial Mousavi, , , Jing Li, , , Nina Filippone, , , Lindsey Walter, , , Han-Wen Cheng, , , Zakiya Skeete, , , Hallie Feldman, , , Sydney Hakimi, , , Kaylee Cappuccio, , , Shan Wang, , , Melissa Hader, , , Guojun Shang, , , James Turner, , , Andrew Cameron, , , Susan Bane, , , Mark Poliks, , , Susan Lu, , , Tony T. Yuan, , and , Chuan-Jian Zhong*, \",\"doi\":\"10.1021/acs.analchem.5c02355\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >The development of highly sensitive detection methodologies for infectious diseases has become increasingly important, considering the recent COVID-19 pandemic. In this report, we demonstrate the use of a gold nanoprobe-based surface-enhanced Raman scattering (SERS) technique as a biosensor for the sensitive detection of SARS-CoV-2, including spike proteins, nucleocapsid proteins, as well as original or heat-inactivated patient samples. The nanoprobes are labeled with Raman reporter molecules for diagnostic detection and quantitative analysis of the protein or virus concentrations in the samples. Key to the detection is the capture of the targeted biomolecules by specific antibody-conjugated gold nanoparticles in a high-surface-area fibrous paper substrate, which facilitates the formation of “hot spots” for SERS signal amplification. 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Plasmonic Nanoprobe-Enabled SERS Detection of SARS-CoV-2 Proteins and Virus Samples on Wax-Printed Paper Substrates
The development of highly sensitive detection methodologies for infectious diseases has become increasingly important, considering the recent COVID-19 pandemic. In this report, we demonstrate the use of a gold nanoprobe-based surface-enhanced Raman scattering (SERS) technique as a biosensor for the sensitive detection of SARS-CoV-2, including spike proteins, nucleocapsid proteins, as well as original or heat-inactivated patient samples. The nanoprobes are labeled with Raman reporter molecules for diagnostic detection and quantitative analysis of the protein or virus concentrations in the samples. Key to the detection is the capture of the targeted biomolecules by specific antibody-conjugated gold nanoparticles in a high-surface-area fibrous paper substrate, which facilitates the formation of “hot spots” for SERS signal amplification. The relationships between the SERS intensity and sample concentration were determined to assess diagnostic sensitivity and limits of detection. The results were also validated using the polymerase chain reaction (PCR) cycle threshold (CT) value vs the concentration of SARS-CoV-2. The nanoprobe-based and fibrous paper-assisted approach demonstrated a low-cost and portable platform technology for enhanced SERS detection of SARS-CoV-2, showing promise for expansion to the detection of various viruses.
期刊介绍：
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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