原位合成卟啉锆mof衍生的超灵敏免疫传感器多层纳米复合探针

IF 8.2 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry: X Pub Date : 2025-09-20 DOI:10.1016/j.fochx.2025.103059

Xiangrui Li , Huan Lv , Xinyu Zhang , Wenjia Yang , Wencan Luo , Linghong Kong , Lu Zeng , Qiujin Zhu

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引用次数: 0

摘要

黄曲霉毒素B1 （AFB1）由于其广泛的污染和潜在的危害而引起了人们的关注。含金纳米颗粒（AuNPs）的比色横向流动免疫分析法（LFIAs）广泛用于现场分析物检测。然而，由于aunp的亮度有限，它仍然具有挑战性。在此，我们介绍了一种用于高灵敏度AFB1检测的免疫传感器，利用多层纳米复合探针（PCN-224@Fe3O4@PDA@Ab/HRP），该探针是通过在卟啉金属-有机框架上依次修饰Fe3O4纳米颗粒、聚多巴胺层和辣根过氧化物酶而制成的，具有磁分离、高信号亮度和强催化活性。与竞争分析格式集成，该免疫传感器实现了AFB1的超灵敏检测，检测范围为0.003-3 ng/mL，检测限（LOD）为0.0014 ng/mL。此外，我们的免疫传感器显示了令人满意的回收率，并成功地检测了谷物样品中的AFB1。因此，这项工作为设计多功能纳米复合探针提供了创新策略，提高了检测灵敏度，在食品安全监测中具有很大的前景。

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In-situ synthesized porphyrinic zirconium MOF-derived multilayered nanocomposite probe for ultra-sensitive immunosensor

Aflatoxin B₁ (AFB₁) is a major concern due to its widespread contamination and potential harm. Colorimetric lateral flow immunoassays (LFIAs) incorporating gold nanoparticles (AuNPs) are widely used for on-site analyte detection. However, it remains challenging due to the limited brightness of AuNPs. Herein, we introduce an immunosensor for highly sensitive AFB₁ detection utilizing a multilayered nanocomposite probe (PCN-224@Fe₃O₄@PDA@Ab/HRP), created by sequentially modifying Fe₃O₄ nanoparticles, polydopamine layers, and horseradish peroxidase on a porphyrinic metal-organic framework, which offers magnetic separation, high signal brightness, and strong catalytic activity. Integrated with a competitive assay format, the immunosensor achieved ultrasensitive detection of AFB₁, with a detection range of 0.003–3 ng/mL and a limit of detection (LOD) of 0.0014 ng/mL. Moreover, our immunosensor exhibited satisfactory recoveries and successful detection of AFB₁ in cereal samples. Therefore, this work offers innovative strategies for designing multi-functional nanocomposite probes that enhance detection sensitivity, holding great promise in food safety monitoring.

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来源期刊

Food Chemistry: X CHEMISTRY, APPLIED-

CiteScore

4.90

自引率

6.60%

发文量

315

审稿时长

55 days

期刊介绍： Food Chemistry: X, one of three Open Access companion journals to Food Chemistry, follows the same aims, scope, and peer-review process. It focuses on papers advancing food and biochemistry or analytical methods, prioritizing research novelty. Manuscript evaluation considers novelty, scientific rigor, field advancement, and reader interest. Excluded are studies on food molecular sciences or disease cure/prevention. Topics include food component chemistry, bioactives, processing effects, additives, contaminants, and analytical methods. The journal welcome Analytical Papers addressing food microbiology, sensory aspects, and more, emphasizing new methods with robust validation and applicability to diverse foods or regions.