牛尿和精液作为博格彼得钩端螺旋体血清型的潜在传播媒介。

IF 4.4 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Camila Hamond, Nathan E Stone, Ellie J Putz, Luis G V Fernandes, Tammy Anderson, Patrick Camp, Jessica Hicks, Tod Stuber, Hans van der Linden, Priscyla Dos Santos Ribeiro, Darrell O Bayles, Jason W Sahl, David M Wagner, Jarlath E Nally
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引用次数: 0

摘要

牛钩端螺旋体病可导致不孕、流产、胎盘炎、后代虚弱、死产和产奶量减少。全球牛钩端螺旋体病的主要原因是博格彼得钩端螺旋体血清型哈卓氏。患有钩端螺旋体病的无症状牛是感染的宿主,可在数月至数年内释放传染性钩端螺旋体。细菌定植的部位包括奶牛和公牛的肾脏或生殖道,或两者都有。在这项观察性研究中,我们调查了一群无症状公牛的钩端螺旋体病;采用lipL32实时荧光定量PCR (rtPCR)检测,24.1%(14/58)患者经尿排出钩体。选择rtpcr阳性公牛尿液样本,通过培养证实钩端螺旋体的生存能力。鉴于牛精液也可能作为疾病传播媒介,我们调查了冷冻精液样本,寻找钩端螺旋体的证据;1.5%(4/268)为lipL32 rtpcr阳性,其中1例为培养阳性。所有从公牛尿液或精液中培养的分离株均归为博格彼得氏乳杆菌Sejroe serovar Hardjo血清群。在钩端螺旋体病仓鼠模型中证实了分离精液的生存力和传染性。我们的研究结果首次报道了直接从冷冻精液中培养血清型Hardjo的能力,并强调了牛尿液和精液在牛钩端螺旋体病传播中的潜在作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bull urine and semen as potential vectors of disease transmission for Leptospira borgpetersenii serovar Hardjo.

Bovine leptospirosis can result in infertility, abortion, placentitis, weak offspring, stillbirths, and decreased milk production. The leading cause of bovine leptospirosis globally is Leptospira borgpetersenii serovar Hardjo. Asymptomatic cattle with leptospirosis serve as reservoir hosts of infection that can shed infectious leptospires for months to years. Sites of bacterial colonization include the kidney or the genital tract, or both, in both cows and bulls. In this observational study, we investigated leptospirosis in a herd of asymptomatic bulls; 24.1% (14/58) were shedding leptospires via urine as confirmed by lipL32 real-time PCR (rtPCR). The viability of leptospires in selected rtPCR-positive bull urine samples was confirmed by culture. Given that bovine semen may also act as a vector of disease transmission, we investigated frozen semen samples for evidence of leptospires; 1.5% (4/268) were lipL32 rtPCR-positive, and, of these, one was culture-positive. All isolates cultured from bull urine or semen were classified as L. borgpetersenii serogroup Sejroe serovar Hardjo. The viability and infectivity of the semen isolate were confirmed in the hamster model of leptospirosis. Our results report for the first time the ability to culture serovar Hardjo directly from frozen semen and highlight the potential role for bull urine and semen in the transmission of bovine leptospirosis.

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来源期刊
Journal of Dairy Science
Journal of Dairy Science 农林科学-奶制品与动物科学
CiteScore
7.90
自引率
17.10%
发文量
784
审稿时长
4.2 months
期刊介绍: The official journal of the American Dairy Science Association®, Journal of Dairy Science® (JDS) is the leading peer-reviewed general dairy research journal in the world. JDS readers represent education, industry, and government agencies in more than 70 countries with interests in biochemistry, breeding, economics, engineering, environment, food science, genetics, microbiology, nutrition, pathology, physiology, processing, public health, quality assurance, and sanitation.
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