泌乳中期奶牛胃灌胃二十二碳六烯酸改变循环白细胞谱,影响对体外细菌刺激的免疫反应。

IF 4.4 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
H L Reisinger, M L Hoorman, K C Krogstad, L K Mamedova, D Vocelle, G A Contreras, A L Lock, B J Bradford
{"title":"泌乳中期奶牛胃灌胃二十二碳六烯酸改变循环白细胞谱,影响对体外细菌刺激的免疫反应。","authors":"H L Reisinger, M L Hoorman, K C Krogstad, L K Mamedova, D Vocelle, G A Contreras, A L Lock, B J Bradford","doi":"10.3168/jds.2025-26951","DOIUrl":null,"url":null,"abstract":"<p><p>n-3 Fatty acids have anti-inflammatory effects across species, and within the n-3 fatty acid family, different fatty acids have unique effects on immune responses. The objective of this study was to assess circulating leukocyte phenotypes and their ex vivo immune response to challenge following abomasal infusions of the n-3 fatty acid docosahexaenoic acid (DHA; C22:6). Eight mid-lactation Holstein cows (97 ± 37 DIM) were randomly assigned to treatment sequence in a 4 × 4 Latin square design. Treatments were 0, 2, 4, and 6 g/d DHA (supplied via an enriched oil containing 65% DHA) infused into the abomasum for 11 d. Blood samples were collected on the final 2 d of each treatment period. Circulating leukocytes and phenotypes were first assessed in the absence of any challenge. Additionally, whole blood was stimulated with Escherichia coli or Staphylococcus aureus bioparticles in the presence of dihydrorhodamine 123 to assess bacterial uptake and oxidative burst of neutrophils by flow cytometry. A separate whole-blood sample was stimulated with heat-killed S. aureus and plasma was collected to quantify cytokine concentrations. Increasing DHA dose reduced circulating eosinophil concentrations in a linear manner and tended to affect lymphocyte concentrations in a quadratic manner. Cytotoxic T and helper T cell populations, along with the abundance of L-selectin on these populations, showed quadratic effects, with peak abundance in cows receiving 2 to 4 g/d DHA. The γδ T cells responded in a cubic manner, with peak concentrations in cows receiving the 4 g/d DHA dose, whereas L-selectin expression in γδ T cells linearly decreased with increasing DHA dose. Unstimulated blood samples were affected by DHA dose in a cubic manner for IL-1α and VEGF-A and a quadratic manner for TNF and IL-36RA, where highest concentrations were observed at 2 g/d DHA. During whole-blood stimulation with S. aureus, MCP-1 concentration increased linearly with increasing DHA dose and TNF responded in a cubic manner, with a nadir at the 6 g/d DHA dose. Following whole-blood stimulation, DHA dose linearly increased the proportion of neutrophils with uptake of E. coli bioparticles, whereas DHA dose did not affect the proportion of neutrophils with uptake of S. aureus. These results indicate that despite the anti-inflammatory effects of DHA, neutrophil response is not blunted following bacterial stimulation. Further studies are required to assess the effects of DHA on in vivo immune responses.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":4.4000,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Abomasal infusion of docosahexaenoic acid to mid-lactation cows alters circulating leukocyte profiles and affects immune response to ex vivo bacterial stimulation.\",\"authors\":\"H L Reisinger, M L Hoorman, K C Krogstad, L K Mamedova, D Vocelle, G A Contreras, A L Lock, B J Bradford\",\"doi\":\"10.3168/jds.2025-26951\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>n-3 Fatty acids have anti-inflammatory effects across species, and within the n-3 fatty acid family, different fatty acids have unique effects on immune responses. The objective of this study was to assess circulating leukocyte phenotypes and their ex vivo immune response to challenge following abomasal infusions of the n-3 fatty acid docosahexaenoic acid (DHA; C22:6). Eight mid-lactation Holstein cows (97 ± 37 DIM) were randomly assigned to treatment sequence in a 4 × 4 Latin square design. Treatments were 0, 2, 4, and 6 g/d DHA (supplied via an enriched oil containing 65% DHA) infused into the abomasum for 11 d. Blood samples were collected on the final 2 d of each treatment period. Circulating leukocytes and phenotypes were first assessed in the absence of any challenge. Additionally, whole blood was stimulated with Escherichia coli or Staphylococcus aureus bioparticles in the presence of dihydrorhodamine 123 to assess bacterial uptake and oxidative burst of neutrophils by flow cytometry. A separate whole-blood sample was stimulated with heat-killed S. aureus and plasma was collected to quantify cytokine concentrations. Increasing DHA dose reduced circulating eosinophil concentrations in a linear manner and tended to affect lymphocyte concentrations in a quadratic manner. Cytotoxic T and helper T cell populations, along with the abundance of L-selectin on these populations, showed quadratic effects, with peak abundance in cows receiving 2 to 4 g/d DHA. The γδ T cells responded in a cubic manner, with peak concentrations in cows receiving the 4 g/d DHA dose, whereas L-selectin expression in γδ T cells linearly decreased with increasing DHA dose. Unstimulated blood samples were affected by DHA dose in a cubic manner for IL-1α and VEGF-A and a quadratic manner for TNF and IL-36RA, where highest concentrations were observed at 2 g/d DHA. During whole-blood stimulation with S. aureus, MCP-1 concentration increased linearly with increasing DHA dose and TNF responded in a cubic manner, with a nadir at the 6 g/d DHA dose. Following whole-blood stimulation, DHA dose linearly increased the proportion of neutrophils with uptake of E. coli bioparticles, whereas DHA dose did not affect the proportion of neutrophils with uptake of S. aureus. These results indicate that despite the anti-inflammatory effects of DHA, neutrophil response is not blunted following bacterial stimulation. Further studies are required to assess the effects of DHA on in vivo immune responses.</p>\",\"PeriodicalId\":354,\"journal\":{\"name\":\"Journal of Dairy Science\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2025-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Dairy Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3168/jds.2025-26951\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Dairy Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3168/jds.2025-26951","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0

摘要

n-3脂肪酸具有跨物种的抗炎作用,在n-3脂肪酸家族中,不同的脂肪酸对免疫反应有独特的作用。本研究的目的是评估循环白细胞表型及其体外免疫应答在胃内注入n-3脂肪酸二十二碳六烯酸(DHA; C22:6)后的挑战。选取8头泌乳中期荷斯坦奶牛(97±37 DIM),采用4 × 4拉丁方设计,随机分为处理顺序。将0、2、4和6 g/d DHA(通过含有65% DHA的富集油提供)注入皱胃,持续11 d。在每个处理期的最后2 d采集血样。在没有任何挑战的情况下,首先评估循环白细胞和表型。此外,用大肠杆菌或金黄色葡萄球菌生物颗粒刺激全血,在存在二氢达拉明123的情况下,通过流式细胞术评估细菌对中性粒细胞的摄取和氧化爆发。用热杀灭金黄色葡萄球菌刺激单独的全血样本,并收集血浆以定量细胞因子浓度。增加DHA剂量降低循环嗜酸性粒细胞浓度呈线性关系,并倾向于以二次关系影响淋巴细胞浓度。细胞毒性T细胞群和辅助T细胞群以及l -选择素在这些群体中的丰度呈二次效应,在奶牛接受2 ~ 4 g/d DHA时丰度达到峰值。γδ T细胞呈立方反应,在DHA剂量为4 g/d的奶牛中,l -选择素的表达量达到峰值,而γδ T细胞中l -选择素的表达量随着DHA剂量的增加呈线性下降。未刺激的血液样本受到DHA剂量的影响,IL-1α和VEGF-A呈立方方式,TNF和IL-36RA呈二次方式,其中最高浓度为2g /d DHA。在金黄色葡萄球菌全血刺激期间,MCP-1浓度随着DHA剂量的增加而线性增加,TNF呈立方反应,在6 g/d DHA剂量时达到最低点。在全血刺激后,DHA剂量线性增加中性粒细胞与大肠杆菌生物颗粒摄取的比例,而DHA剂量不影响中性粒细胞与金黄色葡萄球菌摄取的比例。这些结果表明,尽管DHA具有抗炎作用,但中性粒细胞的反应在细菌刺激后不会减弱。需要进一步的研究来评估DHA对体内免疫反应的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Abomasal infusion of docosahexaenoic acid to mid-lactation cows alters circulating leukocyte profiles and affects immune response to ex vivo bacterial stimulation.

n-3 Fatty acids have anti-inflammatory effects across species, and within the n-3 fatty acid family, different fatty acids have unique effects on immune responses. The objective of this study was to assess circulating leukocyte phenotypes and their ex vivo immune response to challenge following abomasal infusions of the n-3 fatty acid docosahexaenoic acid (DHA; C22:6). Eight mid-lactation Holstein cows (97 ± 37 DIM) were randomly assigned to treatment sequence in a 4 × 4 Latin square design. Treatments were 0, 2, 4, and 6 g/d DHA (supplied via an enriched oil containing 65% DHA) infused into the abomasum for 11 d. Blood samples were collected on the final 2 d of each treatment period. Circulating leukocytes and phenotypes were first assessed in the absence of any challenge. Additionally, whole blood was stimulated with Escherichia coli or Staphylococcus aureus bioparticles in the presence of dihydrorhodamine 123 to assess bacterial uptake and oxidative burst of neutrophils by flow cytometry. A separate whole-blood sample was stimulated with heat-killed S. aureus and plasma was collected to quantify cytokine concentrations. Increasing DHA dose reduced circulating eosinophil concentrations in a linear manner and tended to affect lymphocyte concentrations in a quadratic manner. Cytotoxic T and helper T cell populations, along with the abundance of L-selectin on these populations, showed quadratic effects, with peak abundance in cows receiving 2 to 4 g/d DHA. The γδ T cells responded in a cubic manner, with peak concentrations in cows receiving the 4 g/d DHA dose, whereas L-selectin expression in γδ T cells linearly decreased with increasing DHA dose. Unstimulated blood samples were affected by DHA dose in a cubic manner for IL-1α and VEGF-A and a quadratic manner for TNF and IL-36RA, where highest concentrations were observed at 2 g/d DHA. During whole-blood stimulation with S. aureus, MCP-1 concentration increased linearly with increasing DHA dose and TNF responded in a cubic manner, with a nadir at the 6 g/d DHA dose. Following whole-blood stimulation, DHA dose linearly increased the proportion of neutrophils with uptake of E. coli bioparticles, whereas DHA dose did not affect the proportion of neutrophils with uptake of S. aureus. These results indicate that despite the anti-inflammatory effects of DHA, neutrophil response is not blunted following bacterial stimulation. Further studies are required to assess the effects of DHA on in vivo immune responses.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Dairy Science
Journal of Dairy Science 农林科学-奶制品与动物科学
CiteScore
7.90
自引率
17.10%
发文量
784
审稿时长
4.2 months
期刊介绍: The official journal of the American Dairy Science Association®, Journal of Dairy Science® (JDS) is the leading peer-reviewed general dairy research journal in the world. JDS readers represent education, industry, and government agencies in more than 70 countries with interests in biochemistry, breeding, economics, engineering, environment, food science, genetics, microbiology, nutrition, pathology, physiology, processing, public health, quality assurance, and sanitation.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信