Nicholas G Tolman, Marina Simón, Felicia A Juarez, Chi Zhang, Zhivka I Hristova, Tionna B Ouellette, Michael A Sellarole, Wilhelmine N deVries, Christa Montgomery, Simon W M John
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Absence of Glaucoma in Tg-MYOCY437H Mice of Diverse Genetic Backgrounds.
Purpose: Mutations in the myocilin (MYOC) gene cause elevated intraocular pressure and glaucoma. To better understand the factors influencing susceptibility to glaucoma, we studied the MYOC mutation (MYOCY437H).
Methods: We characterized ocular phenotypes in Tg-MYOCY437H mice on nine different mouse strain backgrounds.
Results: No glaucoma-related phenotypes were observed. We detected neither elevated intraocular pressure (daytime readings) nor optic nerve degeneration differences between wild type (WT) and Tg-MYOCY437H mice. This included an absence of Tg-MYOCY437H-induced phenotypes on a mixed B6SJL background that best reflected the original publications with this strain. We confirmed that this result was not due to an absence of transgene expression in ocular tissues.
Conclusions: Our data indicate undefined complexity and that the previously reported glaucoma phenotypes are not robust across all institutions and environments.
期刊介绍：
Investigative Ophthalmology & Visual Science (IOVS), published as ready online, is a peer-reviewed academic journal of the Association for Research in Vision and Ophthalmology (ARVO). IOVS features original research, mostly pertaining to clinical and laboratory ophthalmology and vision research in general.
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