毛蕊异黄酮通过减轻氧化应激和促进血管生成加速糖尿病大鼠伤口愈合。

IF 2 4区 生物学 Q3 CELL BIOLOGY
Wei Lu, Min Lu, Lifen Xue, Mi Zhou, Meifeng Zhang, Huifeng Zhu
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引用次数: 0

摘要

背景:血管生成是糖尿病创面愈合的一个生理过程。尽管有报道称毛蕊异黄酮对糖尿病肾病具有保护作用,但其在糖尿病创面愈合中的作用和机制尚不清楚。本研究探讨毛蕊异黄酮对糖尿病大鼠创面愈合和血管生成的影响,以及Nrf2/HO-1通路在减轻氧化应激中的作用。方法:在体内,采用高脂饮食6周联合单次腹腔注射链脲佐菌素(STZ) 45 mg/kg诱导SD大鼠2型糖尿病(T2DM)。麻醉后的糖尿病大鼠背部皮肤全切除后给予毛蕊异黄酮治疗2周,观察毛蕊异黄酮对糖尿病创面Nrf2/HO-1通路氧化应激的保护作用。在体外,高糖诱导人脐静脉血管内皮细胞(HUVECs)损伤,然后用毛囊异黄酮或联合Nrf2激动剂处理,以评估毛囊异黄酮是否通过Nrf2/HO-1途径影响细胞活性并抑制氧化损伤。结果:我们的研究结果表明,毛蕊异黄酮通过激活Nrf2/HO-1信号通路,上调下游抗氧化基因,促进血管生成,从而加速伤口愈合。体外研究也表明Nrf2/HO-1信号激活可以增强毛蕊异黄酮对高糖诱导的HUVECs细胞活性的促进作用和对氧化应激的抑制作用。结论:本研究结果表明毛蕊异黄酮可通过Nrf2/HO-1通路促进血管生成、抑制氧化应激等途径促进创面愈合,为治疗难治性糖尿病创面提供理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Calycosin accelerates wound healing in diabetic rats by alleviating oxidative stress and promoting angiogenesis.

Background: Angiogenesis is a physiological process of diabetic wound healing. Although calycosin has been reported to exert protective effects on diabetic nephropathy, its role and mechanisms in diabetic wound healing remain unclear. This study investigates the effects of calycosin on wound healing and angiogenesis, and the role of the Nrf2/HO-1 pathway in mitigating oxidative stress in diabetic rats.

Methods: In vivo, type 2 diabetes (T2DM) in Sprague-Dawley (SD) rats was induced by a high-fat diet for six weeks combined with a single intraperitoneal injection of 45 mg/kg streptozotocin (STZ). The anesthetized diabetic rats underwent a full skin excision on the back and were then treated with calycosin for two weeks to evaluate the protective effect of calycosin on oxidative stress associated with the Nrf2/HO-1 pathway in diabetic wound rats. In vitro, damage to Human Umbilical Vein Vascular Endothelial Cells (HUVECs) was induced by high glucose, and then treated with calycosin or combined with Nrf2 agonist to evaluate whether calycosin affects cell activity and inhibits oxidative damage via the Nrf2/HO-1 pathway.

Results: Our results indicate that calycosin promotes angiogenesis by activating the Nrf2/HO-1 signaling pathway and upregulating downstream antioxidant genes, thereby accelerating wound healing. In vitro studies have also shown that Nrf2/HO-1 signaling activation can enhance the promoting effect of calycosin on cell activity and the inhibitory effect on oxidative stress in HUVECs induced by high glucose.

Conclusion: Our results show that calycosin can accelerate wound healing by promoting angiogenesis and inhibiting oxidative stress mediated by the Nrf2/HO-1 pathway, which provides a theoretical basis for the treatment of refractory diabetic wounds.

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来源期刊
Histology and histopathology
Histology and histopathology 生物-病理学
CiteScore
3.90
自引率
0.00%
发文量
232
审稿时长
2 months
期刊介绍: HISTOLOGY AND HISTOPATHOLOGY is a peer-reviewed international journal, the purpose of which is to publish original and review articles in all fields of the microscopical morphology, cell biology and tissue engineering; high quality is the overall consideration. Its format is the standard international size of 21 x 27.7 cm. One volume is published every year (more than 1,300 pages, approximately 90 original works and 40 reviews). Each volume consists of 12 numbers published monthly online. The printed version of the journal includes 4 books every year; each of them compiles 3 numbers previously published online.
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