脂噬通过优先靶向牛乳腺上皮细胞中的小脂滴改变细胞内甘油三酯的分解代谢。

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-09-13 DOI:10.1002/jsfa.70186

Fangyuan Kang, Jingna Yang, Yuxin Fan, Yanbin Yang, Ying Qian Han, Hongyu Dai, Liqiang Han

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引用次数: 0

摘要

背景：牛奶中脂肪球的大小影响牛奶的质量，主要受牛乳腺上皮细胞中脂滴（ld）大小的影响。本研究旨在探讨脂质摄食如何影响乳腺上皮细胞LDs的分解代谢。结果：用无血清培养基饥饿24 h后开始脂噬。与对照组（CT）相比，细胞内甘油三酯和黄嘌呤脱氢酶（XDH）蛋白水平较低，而过氧化物酶体增殖体激活受体γ (PPAR γ)和胆固醇调节元件结合蛋白1 （SREBP1）在饥饿反应中的表达较高。在饥饿条件下，液晶屏的数量减少，而液晶屏的平均直径和大液晶屏（直径bbb30 μm）的分布增加。结论：本研究提示，营养缺乏诱导自噬体形成，自噬体选择性地将小脂滴传递给溶酶体降解，影响牛上皮细胞甘油三酯的分解代谢。©2025化学工业协会。

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Lipophagy modifies the catabolism of intracellular triglycerides by preferentially targeting small-sized lipid droplets in bovine mammary epithelial cells.

Background: The size of fat globules in milk, which is influenced mainly by the size of lipid droplets (LDs) in bovine mammary epithelial cells, affects the quality of the milk. The present study aimed to explore how lipophagy affected the catabolism of LDs in bovine mammary epithelial cells.

Results: Lipophagy was initiated by 24 h of starvation with serum-free culture medium. Compared with those in the control group (CT), the level of intracellular triglycerides and xanthine dehydrogenase (XDH) protein were lower, whereas the expression of perilipin 2 (PLIN2), peroxisome proliferator-activated receptor gamma (PPAR γ) and sterol regulatory element-binding protein 1 (SREBP1) was greater in response to starvation. The number of LDs was lower, whereas the average diameter of LDs and the distribution of large LDs (diameter > 3 μm) was greater in response to starvation. Inhibition of the lipophagic flux induced the accumulation of small LDs (diameter < 1 μm). Starvation enhanced the expression of adipose triglyceride lipase (ATGL), the inhibition of which increased average diameter of LDs and the accumulation of large LDs. Furthermore, colocalization of ATGL and large LDs was observed in cells subjected to starvation. Inhibition of lysosomal acid lipase decreased the size of LDs, and the colocalization of small LDs and autolysosomes was also observed in bovine mammary epithelial cells subjected to starvation.

Conclusion: The results of the present study suggested that nutrient deficiency induced the formation of autophagosomes, which selectively delivered small lipid droplets to lysosomes for degradation and affected the catabolism of triglycerides in bovine epithelial cells. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.