利用核酸扩增技术检测食源性致病菌的微流控平台研究进展

IF 15.4 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Trends in Food Science & Technology Pub Date : 2025-09-09 DOI:10.1016/j.tifs.2025.105286

Qian Yang , Jinqi Liu , Qi He , Shuaihua Zhang , Longjiao Zhu , Wenqiang Zhang , Dandan Han , Wentao Xu

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引用次数: 0

摘要

背景食源性病原体继续构成重大的公共卫生风险，突出表明需要先进的检测方法来克服传统技术的局限性。菌落计数和ELISA等传统方法受分析时间长、劳动密集型程序和低特异性的限制。尽管核酸扩增技术具有高灵敏度和特异性，但其广泛采用仍然受到复杂仪器和专业操作技能要求的限制。将这些技术集成到微流体系统中，为快速、便携、高效的病原体检测提供了一条有希望的途径，解决了实际实施中的关键挑战。本综述研究了热循环和等温扩增方法，特别强调了三个关键的技术维度：(1)实现精确流体控制的微流体架构，(2)优化芯片上扩增的策略，以及(3)简化工作流程，旨在最大限度地减少分析时间和提高操作的便利性。通过对敏感性、特异性和处理时间的综合评估，我们强调了这些集成系统如何实现便携式、即时诊断解决方案，以解决当前食品安全监测中的局限性。微流控平台与核酸扩增技术的协同整合已经彻底改变了病原体检测，能够在临床相关浓度下敏感识别各种食源性病原体，同时保持菌株分化的特殊特异性。这些系统还提供了区分活细菌和死细菌的潜力。未来的发展将集中在新型微流控芯片材料、优化的扩增酶以及在食品安全方面的更广泛应用。这些技术与纳米技术和人工智能的结合有望进一步增强实时、现场食品安全监测能力。

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Recent advances in microfluidic platforms utilizing nucleic acid amplification technology for the detection of foodborne pathogens

Background

Foodborne pathogens continue to pose significant public health risks, highlighting the requirement for advanced detection methods that overcome the limitations of conventional techniques. Traditional approaches such as colony counting and ELISA are limited by long analysis time, labor-intensive procedures, and low specificity. Although nucleic acid amplification technologies provide high sensitivity and specificity, their widespread adoption remains limited by requirements for complex instrumentation and specialized operational expertise. The integration of these technologies into microfluidic systems presents a promising pathway toward rapid, portable, and efficient pathogen detection, addressing key challenges in practical implementation.

Scope and approach

This review examines both thermal cycling and isothermal amplification approaches, with particular emphasis on three critical technological dimensions: (1) microfluidic architectures enabling precise fluid control, (2) strategies for optimizing on-chip amplification, and (3) workflow simplification aimed at minimizing assay time and improving ease of operation. Through comprehensive assessment of sensitivity, specificity, and processing time, we highlight how these integrated systems enable portable, point-of-care diagnostic solutions that address current limitations in food safety monitoring.

Key findings and conclusions

The synergistic integration of microfluidic platforms with nucleic acid amplification technologies has revolutionized pathogen detection, enabling sensitive identification of diverse foodborne pathogens at clinically relevant concentrations while maintaining exceptional specificity for strain differentiation. These systems also offer the potential to distinguish between live and dead bacteria. Future developments will focus on novel microfluidic chip materials, optimized amplification enzymes, and broader applications in food safety. The combination of these technologies with nanotechnology and artificial intelligence is expected to further enhance real-time, on-site food safety monitoring capabilities.

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来源期刊

Trends in Food Science & Technology 工程技术-食品科技

CiteScore

32.50

自引率

2.60%

发文量

322

审稿时长

37 days

期刊介绍： Trends in Food Science & Technology is a prestigious international journal that specializes in peer-reviewed articles covering the latest advancements in technology, food science, and human nutrition. It serves as a bridge between specialized primary journals and general trade magazines, providing readable and scientifically rigorous reviews and commentaries on current research developments and their potential applications in the food industry. Unlike traditional journals, Trends in Food Science & Technology does not publish original research papers. Instead, it focuses on critical and comprehensive reviews to offer valuable insights for professionals in the field. By bringing together cutting-edge research and industry applications, this journal plays a vital role in disseminating knowledge and facilitating advancements in the food science and technology sector.