围产期摄入瘦素可预防年轻代谢性肥胖正常体重大鼠的认知障碍,海马转录组谱改变

IF 4.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Carmen García-Ruano,Andrea Costa,Andreu Palou,Paula Oliver
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引用次数: 0

摘要

生命早期代谢环境显著影响长期认知和代谢健康。本研究研究了暴露于等热量高脂肪饮食(HFD)的年轻大鼠海马和外周血单核细胞(PBMC)的转录组学改变,导致代谢性肥胖,正常体重(MONW)表型。在断奶后11周,将大鼠配对喂养标准组(NW组)或HFD组(MONW组)。另一组(MONW-Lep)在哺乳期补充瘦素,随后补充HFD。海马转录组学分析显示,在MONW组中,与肥胖和认知能力下降相关的通路被破坏,而瘦素的摄入减轻了这种破坏。这与工作记忆(t迷宫测试)的结果一致,在MONW和NW动物中,工作记忆受损,但在MONW- lep动物中保持不变。PBMC转录组学揭示了与海马重叠的基因。值得注意的是,与神经变性、代谢综合征和肥胖相关的Piwil1基因在MONW动物的PBMC中上调,而在MONW- lep动物中则没有上调,这反映了早期海马的变化和瘦素的预防作用。这些发现强调了早期营养对认知健康的影响,瘦素的保护潜力抵消了HFD摄入的影响,以及PBMC作为大脑健康生物标志物的可靠来源的有用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Altered Hippocampal Transcriptomic Profile Reveals Cognitive Impairment in Young Metabolically Obese, Normal-Weight Rats, Prevented by Perinatal Leptin Intake.
Early-life metabolic environment significantly impacts long-term cognitive and metabolic health. This study investigates transcriptomic alterations in the hippocampus and peripheral blood mononuclear cells (PBMC) of young rats exposed to an isocaloric high-fat diet (HFD), resulting in the metabolically obese, normal-weight (MONW) phenotype. Rats were pair-fed either a standard (NW group) or HFD (MONW group) for 11 weeks after weaning. Another group (MONW-Lep) received leptin supplementation during lactation and subsequently HFD. Transcriptomic analysis of the hippocampus showed disruption of pathways linked to obesity and cognitive decline in the MONW group, which were attenuated by leptin intake. This was consistent with the results of working memory (T-maze test), impaired in MONW versus NW, but preserved in MONW-Lep animals. PBMC transcriptomics revealed overlapping genes with the hippocampus. Notably, Piwil1, a gene linked to neurodegeneration, metabolic syndrome and obesity, was up-regulated in PBMC of MONW but not of MONW-Lep animals, reflecting early hippocampal changes and leptin's preventive effect. These findings highlight the influence of early nutrition on cognitive health, the protective potential of leptin counteracting the effects of HFD intake and the usefulness of PBMC as a reliable source of biomarkers of brain health.
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来源期刊
Molecular Nutrition & Food Research
Molecular Nutrition & Food Research 工程技术-食品科技
CiteScore
8.70
自引率
1.90%
发文量
250
审稿时长
1.7 months
期刊介绍: Molecular Nutrition & Food Research is a primary research journal devoted to health, safety and all aspects of molecular nutrition such as nutritional biochemistry, nutrigenomics and metabolomics aiming to link the information arising from related disciplines: Bioactivity: Nutritional and medical effects of food constituents including bioavailability and kinetics. Immunology: Understanding the interactions of food and the immune system. Microbiology: Food spoilage, food pathogens, chemical and physical approaches of fermented foods and novel microbial processes. Chemistry: Isolation and analysis of bioactive food ingredients while considering environmental aspects.
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