{"title":"猕猴桃抑制结直肠癌新生血管的作用及其机制。","authors":"Ziqi Meng, Minyuan Chen, Jiante Li, Jieyu Li","doi":"10.14670/HH-18-977","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Colorectal cancer is one of the most common cancers worldwide, and its angiogenesis is a key factor in tumor growth and metastasis. <i>Radix Actinidiae chinensis</i> is considered to have antitumor activity in traditional Chinese medicine, but its effect on neovascularization in colorectal cancer has not been clarified. Herein, we aimed to evaluate the effect of different concentrations of <i>Radix Actinidiae chinensis</i> on the neovascularization of colorectal cancer and explore its possible mechanisms.</p><p><strong>Method: </strong>A mouse model of colorectal cancer was established, and mice were randomly divided into control, low-, and high-concentration groups. Then the mice in the experimental group were treated with <i>Radix Actinidiae chinensis</i>, and its effects on neovascularization and tumor growth were evaluated by tumor growth curve tracking, immunohistochemical analysis, vessel density assessment, RT-qPCR, and protein immunoblotting to explore the underlying mechanisms.</p><p><strong>Results: </strong>It was shown that tumor tissues in the high-concentration group exhibited significantly slower growth in both mass and volume compared with the low-concentration and control groups. Immunohistochemical staining revealed a reduction in the expression of the vascular endothelial marker CD31 in the <i>Radix Actinidiae chinensis</i> treatment group. Moreover, the protein expression levels of vascular markers in tumor tissues showed a slight decrease in the low-concentration group and a marked reduction in the high-concentration group. These findings suggest that angiogenesis in the tumor microenvironment was inhibited in a concentration-dependent manner, with protein expression levels closely mirroring gene expression patterns.</p><p><strong>Conclusion: </strong>The study found that <i>Radix Actinidiae chinensis</i> inhibits neovascularization in a dose-dependent manner in a mouse model of colorectal cancer. These results provide experimental support for its potential use as a therapeutic agent against colorectal cancer, suggesting that it may suppress tumor growth and metastasis by inhibiting angiogenesis.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"18977"},"PeriodicalIF":2.0000,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Radix Actinidiae chinensis</i> inhibits neovascularization in colorectal cancer and its mechanism.\",\"authors\":\"Ziqi Meng, Minyuan Chen, Jiante Li, Jieyu Li\",\"doi\":\"10.14670/HH-18-977\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Colorectal cancer is one of the most common cancers worldwide, and its angiogenesis is a key factor in tumor growth and metastasis. <i>Radix Actinidiae chinensis</i> is considered to have antitumor activity in traditional Chinese medicine, but its effect on neovascularization in colorectal cancer has not been clarified. Herein, we aimed to evaluate the effect of different concentrations of <i>Radix Actinidiae chinensis</i> on the neovascularization of colorectal cancer and explore its possible mechanisms.</p><p><strong>Method: </strong>A mouse model of colorectal cancer was established, and mice were randomly divided into control, low-, and high-concentration groups. Then the mice in the experimental group were treated with <i>Radix Actinidiae chinensis</i>, and its effects on neovascularization and tumor growth were evaluated by tumor growth curve tracking, immunohistochemical analysis, vessel density assessment, RT-qPCR, and protein immunoblotting to explore the underlying mechanisms.</p><p><strong>Results: </strong>It was shown that tumor tissues in the high-concentration group exhibited significantly slower growth in both mass and volume compared with the low-concentration and control groups. Immunohistochemical staining revealed a reduction in the expression of the vascular endothelial marker CD31 in the <i>Radix Actinidiae chinensis</i> treatment group. Moreover, the protein expression levels of vascular markers in tumor tissues showed a slight decrease in the low-concentration group and a marked reduction in the high-concentration group. These findings suggest that angiogenesis in the tumor microenvironment was inhibited in a concentration-dependent manner, with protein expression levels closely mirroring gene expression patterns.</p><p><strong>Conclusion: </strong>The study found that <i>Radix Actinidiae chinensis</i> inhibits neovascularization in a dose-dependent manner in a mouse model of colorectal cancer. These results provide experimental support for its potential use as a therapeutic agent against colorectal cancer, suggesting that it may suppress tumor growth and metastasis by inhibiting angiogenesis.</p>\",\"PeriodicalId\":13164,\"journal\":{\"name\":\"Histology and histopathology\",\"volume\":\" \",\"pages\":\"18977\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-09-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Histology and histopathology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.14670/HH-18-977\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Histology and histopathology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.14670/HH-18-977","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Radix Actinidiae chinensis inhibits neovascularization in colorectal cancer and its mechanism.
Objective: Colorectal cancer is one of the most common cancers worldwide, and its angiogenesis is a key factor in tumor growth and metastasis. Radix Actinidiae chinensis is considered to have antitumor activity in traditional Chinese medicine, but its effect on neovascularization in colorectal cancer has not been clarified. Herein, we aimed to evaluate the effect of different concentrations of Radix Actinidiae chinensis on the neovascularization of colorectal cancer and explore its possible mechanisms.
Method: A mouse model of colorectal cancer was established, and mice were randomly divided into control, low-, and high-concentration groups. Then the mice in the experimental group were treated with Radix Actinidiae chinensis, and its effects on neovascularization and tumor growth were evaluated by tumor growth curve tracking, immunohistochemical analysis, vessel density assessment, RT-qPCR, and protein immunoblotting to explore the underlying mechanisms.
Results: It was shown that tumor tissues in the high-concentration group exhibited significantly slower growth in both mass and volume compared with the low-concentration and control groups. Immunohistochemical staining revealed a reduction in the expression of the vascular endothelial marker CD31 in the Radix Actinidiae chinensis treatment group. Moreover, the protein expression levels of vascular markers in tumor tissues showed a slight decrease in the low-concentration group and a marked reduction in the high-concentration group. These findings suggest that angiogenesis in the tumor microenvironment was inhibited in a concentration-dependent manner, with protein expression levels closely mirroring gene expression patterns.
Conclusion: The study found that Radix Actinidiae chinensis inhibits neovascularization in a dose-dependent manner in a mouse model of colorectal cancer. These results provide experimental support for its potential use as a therapeutic agent against colorectal cancer, suggesting that it may suppress tumor growth and metastasis by inhibiting angiogenesis.
期刊介绍:
HISTOLOGY AND HISTOPATHOLOGY is a peer-reviewed international journal, the purpose of which is to publish original and review articles in all fields of the microscopical morphology, cell biology and tissue engineering; high quality is the overall consideration. Its format is the standard international size of 21 x 27.7 cm. One volume is published every year (more than 1,300 pages, approximately 90 original works and 40 reviews). Each volume consists of 12 numbers published monthly online. The printed version of the journal includes 4 books every year; each of them compiles 3 numbers previously published online.