Alanna Botelho, Adrian Chaves Penha, Lucas Tadeu N da Silva Rocha, Ariane Gaspar Santos, Danuza N Moysés, Aline Machado de Castro, Maria Alice Z Coelho, Priscilla F F Amaral
{"title":"使用二甲亚砜（DMSO）提高聚脂耶氏菌IMUFRJ 50682对PET的微生物降解","authors":"Alanna Botelho, Adrian Chaves Penha, Lucas Tadeu N da Silva Rocha, Ariane Gaspar Santos, Danuza N Moysés, Aline Machado de Castro, Maria Alice Z Coelho, Priscilla F F Amaral","doi":"10.1007/s00449-025-03230-5","DOIUrl":null,"url":null,"abstract":"<p><p>Yarrowia lipolytica has been studied for poly (ethylene terephthalate) (PET) depolymerization, but the slow kinetics must be improved for large-scale applications. Here, dimethyl sulfoxide (DMSO) was added to a medium containing post-consumer PET (PC-PET) or the monomers terephthalic acid (TPA), bis(hydroxy-ethylene) terephthalate (BHET), and methyl-2-hydroxy ethylene terephthalate (MHET) to increase its solubility and improve depolymerization. The MIC test indicated 5% of DMSO as the maximum non-toxic concentration for Y. lipolytica cultivation. Cell viability on yeast nitrogen-based (YNB) medium was higher with MHET (94%). Cell growth in YNB medium and PC-PET was only detected with DMSO. When PC-PET was used as an additional carbon source, cell growth was 40% higher in the presence of DMSO (10.7 g/L), exhibiting increased adhesion of cells to PET (20%). Also, the highest extracellular lipase activity (370 U/L) was found with DMSO and PC-PET in flasks. In a bioreactor, higher cell growth (32.6 g/L) and lipase activity (7531 U/L) were obtained in YP*D medium with PC-PET and DMSO. During cultivation in this medium, TPA, MHET, and BHET were detected, demonstrating PET depolymerization along Y. lipolytica growth with DMSO. These results show that DMSO contributes to PET depolymerization by Y. lipolytica, increasing cell concentration, adhesion to PET particles, and enzyme production.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The use of dimethyl sulfoxide (DMSO) to increase PET microbial degradation by Yarrowia lipolytica IMUFRJ 50682.\",\"authors\":\"Alanna Botelho, Adrian Chaves Penha, Lucas Tadeu N da Silva Rocha, Ariane Gaspar Santos, Danuza N Moysés, Aline Machado de Castro, Maria Alice Z Coelho, Priscilla F F Amaral\",\"doi\":\"10.1007/s00449-025-03230-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Yarrowia lipolytica has been studied for poly (ethylene terephthalate) (PET) depolymerization, but the slow kinetics must be improved for large-scale applications. Here, dimethyl sulfoxide (DMSO) was added to a medium containing post-consumer PET (PC-PET) or the monomers terephthalic acid (TPA), bis(hydroxy-ethylene) terephthalate (BHET), and methyl-2-hydroxy ethylene terephthalate (MHET) to increase its solubility and improve depolymerization. The MIC test indicated 5% of DMSO as the maximum non-toxic concentration for Y. lipolytica cultivation. Cell viability on yeast nitrogen-based (YNB) medium was higher with MHET (94%). Cell growth in YNB medium and PC-PET was only detected with DMSO. When PC-PET was used as an additional carbon source, cell growth was 40% higher in the presence of DMSO (10.7 g/L), exhibiting increased adhesion of cells to PET (20%). Also, the highest extracellular lipase activity (370 U/L) was found with DMSO and PC-PET in flasks. In a bioreactor, higher cell growth (32.6 g/L) and lipase activity (7531 U/L) were obtained in YP*D medium with PC-PET and DMSO. During cultivation in this medium, TPA, MHET, and BHET were detected, demonstrating PET depolymerization along Y. lipolytica growth with DMSO. These results show that DMSO contributes to PET depolymerization by Y. lipolytica, increasing cell concentration, adhesion to PET particles, and enzyme production.</p>\",\"PeriodicalId\":9024,\"journal\":{\"name\":\"Bioprocess and Biosystems Engineering\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2025-08-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioprocess and Biosystems Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s00449-025-03230-5\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprocess and Biosystems Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s00449-025-03230-5","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
The use of dimethyl sulfoxide (DMSO) to increase PET microbial degradation by Yarrowia lipolytica IMUFRJ 50682.
Yarrowia lipolytica has been studied for poly (ethylene terephthalate) (PET) depolymerization, but the slow kinetics must be improved for large-scale applications. Here, dimethyl sulfoxide (DMSO) was added to a medium containing post-consumer PET (PC-PET) or the monomers terephthalic acid (TPA), bis(hydroxy-ethylene) terephthalate (BHET), and methyl-2-hydroxy ethylene terephthalate (MHET) to increase its solubility and improve depolymerization. The MIC test indicated 5% of DMSO as the maximum non-toxic concentration for Y. lipolytica cultivation. Cell viability on yeast nitrogen-based (YNB) medium was higher with MHET (94%). Cell growth in YNB medium and PC-PET was only detected with DMSO. When PC-PET was used as an additional carbon source, cell growth was 40% higher in the presence of DMSO (10.7 g/L), exhibiting increased adhesion of cells to PET (20%). Also, the highest extracellular lipase activity (370 U/L) was found with DMSO and PC-PET in flasks. In a bioreactor, higher cell growth (32.6 g/L) and lipase activity (7531 U/L) were obtained in YP*D medium with PC-PET and DMSO. During cultivation in this medium, TPA, MHET, and BHET were detected, demonstrating PET depolymerization along Y. lipolytica growth with DMSO. These results show that DMSO contributes to PET depolymerization by Y. lipolytica, increasing cell concentration, adhesion to PET particles, and enzyme production.
期刊介绍：
Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes.
Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged.
The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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