山茱萸叶中二羟基三萜的研究。

IF 3.6 2区生物学 Q2 CHEMISTRY, MEDICINAL

Journal of Natural Products Pub Date : 2025-08-31 DOI:10.1021/acs.jnatprod.5c00360

Hui-Jiao Yan, , , Ming-Ye Li, , , Jia-Le Zhou, , , Yan-Ling Geng, , and , Xiao Wang*,

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引用次数: 0

摘要

从山茱萸叶中分离得到13个新的3,4-二叔环三萜（1- 11,15,16），1个新的2,3-二叔环三萜（17），1个新的3,4-二叔环-29-非三叔环三萜（12），1个新的3,4-二叔环-28,29-二叔环三萜（13）和1个已知的三叔环类似物（14）。这些三萜化合物的结构是通过光谱方法确定的，包括hresms，核磁共振光谱分析，单晶x射线衍射和ECD数据。细胞毒性评价表明，化合物4和11对HCT-116和DLD-1细胞株具有中等活性，IC50值在3.7 ~ 4.4 μM之间。Annexin V-PE/7-AAD染色结果显示，4和11均呈浓度依赖性诱导细胞凋亡。进一步研究表明，化合物6、9、10和17对lps诱导的RAW 264.7细胞的NO生成有抑制作用，IC50值在17.1±3.3 ~ 49.8±6.5 μM之间。

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seco-Tirucallane Triterpenoids from the Leaves of Dysoxylum gotadhora

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seco-Tirucallane Triterpenoids from the Leaves of Dysoxylum gotadhora

Thirteen new 3,4-seco-tirucallane triterpenoids (1–11, 15, 16), along with one new 2,3-seco-tirucallane triterpenoid (17), one new 3,4-seco-29-nor-tirucallane triterpenoid (12), one new 3,4-seco-28,29-dinor-tirucallane triterpenoid (13), and a known tirucallane analogue (14) were isolated from leaves of Dysoxylum gotadhora. These triterpenoids were structurally determined by spectroscopic methods, including HRESIMS, NMR spectroscopic analyses, single-crystal X-ray diffraction, and ECD data. The cytotoxicity evaluation revealed that compounds 4 and 11 exhibited moderate activity against HCT-116 and DLD-1 cell lines with IC₅₀ values ranging from 3.7 to 4.4 μM. The Annexin V-PE/7-AAD staining assay indicated that both 4 and 11 induced apoptosis in a concentration-dependent manner. Further studies showed that compounds 6, 9, 10, and 17 exhibited suppression effects on NO production in LPS-induced RAW 264.7 cells, with IC₅₀ values ranging from 17.1 ± 3.3 to 49.8 ± 6.5 μM.

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来源期刊

Journal of Natural Products 生物-药学

CiteScore

9.10

自引率

5.90%

发文量

294

审稿时长

2.3 months

期刊介绍： The Journal of Natural Products invites and publishes papers that make substantial and scholarly contributions to the area of natural products research. Contributions may relate to the chemistry and/or biochemistry of naturally occurring compounds or the biology of living systems from which they are obtained. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin. When new compounds are reported, manuscripts describing their biological activity are much preferred. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin.