{"title":"马铃薯StCCoAOMT基因家族的鉴定及StCCoAOMT1和StCCoAOMT7基因对干旱、碱盐及复合胁迫的抗性分析","authors":"Yunyun Du, Ruyan Zhang, Yuan Lu, Yong Wang, Xingxing Wang, Weina Zhang, Yichen Kang, Yuhui Liu, Shuhao Qin","doi":"10.1186/s40538-025-00841-8","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Drought and alkaline salt stress act synergistically on potato, affecting growth and causing reduced yield and quality. Lignin plays a key role in potato resistance to abiotic stresses, and most of the key enzymes in its synthetic pathway were involved in plant stress response. Among them, caffeoyl CoA O-methyltransferase (CCoAOMT) is a key enzyme for G-type lignin synthesis, and the <i>StCCoAOMT</i> gene is involved in potato lignin synthesis and response to plant stress in response to adversity.</p><h3>Results</h3><p>The study identified 13 <i>StCCoAOMT</i> genes, which were classified into four subgroups by evolutionary analysis. We have analyzed their physicochemical properties, gene structures, motifs, and cis-acting elements. <i>StCCoAOMT</i> family genes are subject to purification selection, and tandem repeats are the primary driver of gene duplication. The collinearity relationships with other species analysis showed that the <i>StCCoAOMT</i> genes are evolutionarily distinct from monocotyledonous plants. Through transcriptomic analysis and RT-qPCR validation of the tissue-specific expression patterns of <i>StCCoAOMT</i> genes under drought, alkaline salt, and combined stress conditions, we identified the stress-responsive gene <i>StCCoAOMT7</i>.We also obtained the homologous gene, <i>StCCoAOMT1</i>, which has the highest degree of similarity to the Arabidopsis thaliana gene At4g34050, which is responsive to drought and salt stresses, by sequence comparison.</p><p>VIGS analysis revealed that <i>NbCCoAOMT1</i> and <i>NbCCoAOMT7</i> silenced tobacco plants displayed reduced resistance compared to WT plants under drought, alkaline salt, and combined stress. It is speculated that the <i>StCCoAOMT1</i> and <i>StCCoAOMT7</i> genes positively regulate drought, alkaline salt, and combined stress. The subcellular localization of StCCoAOMT1 and StCCoAOMT7 proteins was investigated in tobacco. The results indicate that both proteins may function in the nucleus, plasma membrane, and cytoplasm, providing new insights into the molecular mechanisms underlying plant defense and stress responses.</p><h3>Conclusions</h3><p><i>StCCoAOMT1</i> and <i>StCCoAOMT7</i> were screened as drought, alkaline salt, and combined stress response genes.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"12 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00841-8","citationCount":"0","resultStr":"{\"title\":\"Identification of StCCoAOMT gene family and analysis of resistance of StCCoAOMT1 and StCCoAOMT7 genes to drought, alkaline salt and combined stresses in potato\",\"authors\":\"Yunyun Du, Ruyan Zhang, Yuan Lu, Yong Wang, Xingxing Wang, Weina Zhang, Yichen Kang, Yuhui Liu, Shuhao Qin\",\"doi\":\"10.1186/s40538-025-00841-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Drought and alkaline salt stress act synergistically on potato, affecting growth and causing reduced yield and quality. Lignin plays a key role in potato resistance to abiotic stresses, and most of the key enzymes in its synthetic pathway were involved in plant stress response. Among them, caffeoyl CoA O-methyltransferase (CCoAOMT) is a key enzyme for G-type lignin synthesis, and the <i>StCCoAOMT</i> gene is involved in potato lignin synthesis and response to plant stress in response to adversity.</p><h3>Results</h3><p>The study identified 13 <i>StCCoAOMT</i> genes, which were classified into four subgroups by evolutionary analysis. We have analyzed their physicochemical properties, gene structures, motifs, and cis-acting elements. <i>StCCoAOMT</i> family genes are subject to purification selection, and tandem repeats are the primary driver of gene duplication. The collinearity relationships with other species analysis showed that the <i>StCCoAOMT</i> genes are evolutionarily distinct from monocotyledonous plants. Through transcriptomic analysis and RT-qPCR validation of the tissue-specific expression patterns of <i>StCCoAOMT</i> genes under drought, alkaline salt, and combined stress conditions, we identified the stress-responsive gene <i>StCCoAOMT7</i>.We also obtained the homologous gene, <i>StCCoAOMT1</i>, which has the highest degree of similarity to the Arabidopsis thaliana gene At4g34050, which is responsive to drought and salt stresses, by sequence comparison.</p><p>VIGS analysis revealed that <i>NbCCoAOMT1</i> and <i>NbCCoAOMT7</i> silenced tobacco plants displayed reduced resistance compared to WT plants under drought, alkaline salt, and combined stress. It is speculated that the <i>StCCoAOMT1</i> and <i>StCCoAOMT7</i> genes positively regulate drought, alkaline salt, and combined stress. The subcellular localization of StCCoAOMT1 and StCCoAOMT7 proteins was investigated in tobacco. The results indicate that both proteins may function in the nucleus, plasma membrane, and cytoplasm, providing new insights into the molecular mechanisms underlying plant defense and stress responses.</p><h3>Conclusions</h3><p><i>StCCoAOMT1</i> and <i>StCCoAOMT7</i> were screened as drought, alkaline salt, and combined stress response genes.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":512,\"journal\":{\"name\":\"Chemical and Biological Technologies in Agriculture\",\"volume\":\"12 1\",\"pages\":\"\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2025-08-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00841-8\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical and Biological Technologies in Agriculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s40538-025-00841-8\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-025-00841-8","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
干旱和碱盐胁迫对马铃薯产生协同作用，影响马铃薯生长，造成产量和品质下降。木质素在马铃薯抗非生物胁迫中起着关键作用，其合成途径中的大部分关键酶都参与了植物的逆境响应。其中，咖啡酰辅酶a o -甲基转移酶（CCoAOMT）是g型木质素合成的关键酶，StCCoAOMT基因在逆境中参与了马铃薯木质素的合成和对植物胁迫的响应。结果共鉴定出13个StCCoAOMT基因，通过进化分析将其分为4个亚群。我们分析了它们的理化性质、基因结构、基序和顺式作用元件。StCCoAOMT家族基因受到纯化选择的影响，串联重复序列是基因复制的主要驱动因素。与其他物种的共线性分析表明，StCCoAOMT基因在进化上不同于单子叶植物。通过转录组学分析和RT-qPCR验证StCCoAOMT基因在干旱、碱盐和复合胁迫条件下的组织特异性表达模式，我们确定了胁迫响应基因StCCoAOMT7。通过序列比对，我们还获得了与拟南芥对干旱和盐胁迫有响应的基因At4g34050相似度最高的同源基因StCCoAOMT1。VIGS分析显示，NbCCoAOMT1和NbCCoAOMT7沉默烟草植株在干旱、碱盐和复合胁迫下的抗性均低于WT植株。推测StCCoAOMT1和StCCoAOMT7基因正调控干旱、碱盐和复合胁迫。研究了StCCoAOMT1和StCCoAOMT7蛋白在烟草中的亚细胞定位。结果表明，这两种蛋白可能在细胞核、质膜和细胞质中发挥作用，为植物防御和胁迫反应的分子机制提供了新的认识。结论stccoaomt1和StCCoAOMT7被筛选为干旱、碱盐和联合胁迫响应基因。图形抽象
Identification of StCCoAOMT gene family and analysis of resistance of StCCoAOMT1 and StCCoAOMT7 genes to drought, alkaline salt and combined stresses in potato
Background
Drought and alkaline salt stress act synergistically on potato, affecting growth and causing reduced yield and quality. Lignin plays a key role in potato resistance to abiotic stresses, and most of the key enzymes in its synthetic pathway were involved in plant stress response. Among them, caffeoyl CoA O-methyltransferase (CCoAOMT) is a key enzyme for G-type lignin synthesis, and the StCCoAOMT gene is involved in potato lignin synthesis and response to plant stress in response to adversity.
Results
The study identified 13 StCCoAOMT genes, which were classified into four subgroups by evolutionary analysis. We have analyzed their physicochemical properties, gene structures, motifs, and cis-acting elements. StCCoAOMT family genes are subject to purification selection, and tandem repeats are the primary driver of gene duplication. The collinearity relationships with other species analysis showed that the StCCoAOMT genes are evolutionarily distinct from monocotyledonous plants. Through transcriptomic analysis and RT-qPCR validation of the tissue-specific expression patterns of StCCoAOMT genes under drought, alkaline salt, and combined stress conditions, we identified the stress-responsive gene StCCoAOMT7.We also obtained the homologous gene, StCCoAOMT1, which has the highest degree of similarity to the Arabidopsis thaliana gene At4g34050, which is responsive to drought and salt stresses, by sequence comparison.
VIGS analysis revealed that NbCCoAOMT1 and NbCCoAOMT7 silenced tobacco plants displayed reduced resistance compared to WT plants under drought, alkaline salt, and combined stress. It is speculated that the StCCoAOMT1 and StCCoAOMT7 genes positively regulate drought, alkaline salt, and combined stress. The subcellular localization of StCCoAOMT1 and StCCoAOMT7 proteins was investigated in tobacco. The results indicate that both proteins may function in the nucleus, plasma membrane, and cytoplasm, providing new insights into the molecular mechanisms underlying plant defense and stress responses.
Conclusions
StCCoAOMT1 and StCCoAOMT7 were screened as drought, alkaline salt, and combined stress response genes.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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