苦荞（Fagopyrum tataricum）过敏原基因fag2的种子特异性启动子及其对植物激素的反应。

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-08-20 DOI:10.1002/jsfa.70122

Bei Zheng, Jiali Lu, Yuwei Zhang, Jingbo Zhang, Peng Chen

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引用次数: 0

摘要

背景：苦荞中的一种主要过敏原Fag t2在籽粒中含量丰富，具有提高拟南芥抗旱性和抗病性的潜力，因此人们假设Fag t2的启动子具有调节种子特异性表达和响应胁迫的功能。结果：本研究利用基因组行走技术成功克隆了fag2启动子。通过基因枪轰击、GUS染色和活性检测对fag2启动子的功能进行了研究。结果表明全长启动子具有种子特异性启动子的作用。随着种子特异性元件的减少，种子表达特异性逐渐降低。全长启动子和截断启动子对植物激素的响应存在差异。然而，启动子的响应性在原生质体和拟蓝藻之间存在差异，表明fag2启动子在稳定转化系统中的调控功能更为复杂。结论：本研究结果提示fag2启动子具有作为种子特异性启动子的潜力，可以激活特定位点功能基因的表达，从而达到提高作物产量和品质的目的。fag2启动子在fag2介导的植物抗逆性中的调控作用有待进一步深入研究。©2025化学工业协会。

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A seed-specific promoter from allergen gene Fag t 2 in Tartary buckwheat (Fagopyrum tataricum) and its responses to phytohormone.

Background: A major allergen Fag t 2 in Tartary buckwheat is abundant in grains and has the potential to improve drought and disease resistance of Arabidopsis thaliana, which led to the hypothesis that the promoter of Fag t 2 had the function of regulating seed-specific expression and responding to stress.

Results: This study successfully cloned the Fag t 2 promoter using genome walking. The functions of the Fag t 2 promoter were investigated through gene gun bombardment, GUS staining and activity detection. The findings revealed that full-length promoter acted as a seed-specific promoter. As seed-specific elements decrease, the seed expression specificity diminishes progressively. The full-length and truncated promoters exhibited differential responses to phytohormone. However, the responsiveness of promoters varied between the protoplast and A. thaliana, suggesting that the regulatory function of the Fag t 2 promoter in stable transformation system is more complicated.

Conclusion: This results of the present study suggest that Fag t 2 promoter has potential as a seed-specific promoter, which can be used to activate the expression of functional genes at specific sites when aiming to improve crop yield and quality. The regulatory function of Fag t 2 promoter in plant stress resistance mediated by Fag t 2 should be explored in greater depth. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.