Exploring the Potential Role of Quercetin Dihydrate Against Carbon Tetrachloride Induced Oxidative Stress in Mice: A Randomized Control Trial

This study investigated the protective efficacy of Quercetin Dihydrate against carbon tetrachloride-(CCl₄) induced oxidative stress using an animal model. A total of 24 healthy male mice were randomly divided into four groups. The first group served as control; the second group was treated with CCl₄ (1 mL/kg b.w) intraperitoneally on day 21, and the remaining two treatment groups received varying daily doses of Quercetin Dihydrate (60, 120 mg/kg b.w) for 20 days and carbon tetrachloride (1 mL/kg b.w) intraperitoneally on day 21. We assayed biomarkers of oxidative stress, malondialdehyde (MDA), and catalase (CAT) in the liver, muscles, and kidneys. Moreover, carbon tetrachloride induces toxicity, which was associated with increased MDA levels (p ≤ 0.05) in the Control Positive group, indicating oxidative stress in mice. Quercetin Dihydrate supplementation decreased the MDA activity in the liver, muscle, and kidneys. The antioxidant activities of CAT were decreased (p ≤ 0.05) after carbon tetrachloride exposure. Quercetin Dihydrate administration normalized CCl₄-induced changes and significantly restored the redox status. In conclusion, by improving the oxidative stress markers, Quercetin Dihydrate exerted significant protection and antioxidant activities against carbon tetrachloride-induced oxidative stress.