Dectin-1通过诱导小胶质细胞介导的炎症和血视网膜屏障破坏驱动糖尿病视网膜病变

IF 2.3 4区 医学 Q2 DEVELOPMENTAL BIOLOGY
Lei Zhang, Sumei Zhang, Yingjun Li, Zhen Yang, Weikang Hu, Hongmei Bai, Wenjing Zhou, Zihan Wang, Mingcong Li, Shengquan Zhang, Rongfeng Liao
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引用次数: 0

摘要

糖尿病视网膜病变(DR)是糖尿病患者的主要表现,是一种严重的视力威胁疾病。Dectin-1作为一种先天免疫受体,已被认为是糖尿病的重要调节因子。在这种情况下,Dectin-1在DR过程中的含义仍然是一个难题,将在这里解决。通过腹腔注射链脲佐菌素(STZ)建立糖尿病小鼠模型,并对人小胶质细胞(HMC3)进行高糖(HG)处理,建立糖尿病细胞模型。记录小鼠的血糖水平和体重。逆转录定量PCR (RT-qPCR)和western blotting检测Dectin-1表达。RT-qPCR、酶联免疫吸附试验(ELISA)和western blotting检测炎症水平。免疫荧光染色和western blotting检测了IBA-1和紧密连接蛋白的表达。western blotting检测白蛋白表达。末端脱氧核苷转移酶介导的缺口末端标记法(TUNEL)和western blotting检测细胞凋亡水平。Dectin-1在糖尿病小鼠视网膜组织和hg暴露的HMC3细胞中均有高表达。Dectin-1拮抗剂laminarin (LAM)在体外和体内均可明显抑制小胶质细胞活化、炎症反应和血视网膜屏障(BRB)渗漏。此外,LAM在体内具有抗凋亡作用。综上所述,Dectin-1抑制剂可能阻断炎症级联反应,防止DR中BRB的破坏。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dectin-1 Drives Diabetic Retinopathy via Inducing Microglia-Mediated Inflammation and Blood–Retinal Barrier Breakdown

Diabetic retinopathy (DR), a prevailing manifestation among diabetic patients, occurs as a major sight-threatening disorder. Dectin-1, as an innate immune receptor, has been notified as a critical modulator of diabetes mellitus. In this context, the implication of Dectin-1 in the process of DR that is still a conundrum will be addressed here. The diabetic mouse model was established by intraperitoneal injection of streptozotocin (STZ), and human microglia cells (HMC3) were subjected to high glucose (HG) to create cellular models of diabetes. Glucose level and body weight were recorded in mice. Reverse transcription-quantitative PCR (RT-qPCR) and western blotting checked Dectin-1 expression. RT-qPCR, enzyme-linked immunosorbent assay (ELISA), and western blotting appraised the inflammatory levels. Immunofluorescence staining and western blotting ascertained the expression of IBA-1 and tight junction proteins. Besides, western blotting also examined albumin expression. Terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) assay and western blotting assayed the apoptotic level. Dectin-1 was highly expressed in both retinal tissues of diabetic mice and HG-exposed HMC3 cells. Dectin-1 antagonist laminarin (LAM) observably repressed microglia activation, inflammatory reaction, and blood–retinal barrier (BRB) leakage both in vitro and in vivo. Moreover, LAM produced anti-apoptotic effect in vivo. To sum up, Dectin-1 inhibitor might block the inflammatory cascade and protect against BRB disruption in DR.

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来源期刊
Developmental Neurobiology
Developmental Neurobiology 生物-发育生物学
CiteScore
6.50
自引率
0.00%
发文量
45
审稿时长
4-8 weeks
期刊介绍: Developmental Neurobiology (previously the Journal of Neurobiology ) publishes original research articles on development, regeneration, repair and plasticity of the nervous system and on the ontogeny of behavior. High quality contributions in these areas are solicited, with an emphasis on experimental as opposed to purely descriptive work. The Journal also will consider manuscripts reporting novel approaches and techniques for the study of the development of the nervous system as well as occasional special issues on topics of significant current interest. We welcome suggestions on possible topics from our readers.
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