Xiuying Tang, Carla J Abbott, Penelope J Allen, Chi D Luu
{"title":"视网膜电图和Celeris系统视觉诱发电位的会话间重复性。","authors":"Xiuying Tang, Carla J Abbott, Penelope J Allen, Chi D Luu","doi":"10.1007/s10633-025-10045-y","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>The Celeris system (Diagnosys LLC) offers a streamlined alternative to the gold-standard Espion system for high-throughput electroretinography (ERG) and visual evoked potentials (VEP) in preclinical studies. This study evaluated its inter-session repeatability of ERG and VEP measurements in healthy rodent retinae.</p><p><strong>Methods: </strong>Twenty-five wild type Brown Norway rats underwent ERG and VEP testing across two sessions. Dark-adapted ERG a- and b-waves (flash stimulus of 10 cd s/m<sup>2</sup>), and the photopic negative response (PhNR) (flash stimulus of 3 cd s/m<sup>2</sup>) were recorded. VEPs (flash stimulus of 3 cd s/m<sup>2</sup>) were recorded from implanted screw electrodes in the skull. Pearson's correlation coefficient (ρ), Bland-Altman with 95% limits of agreement (LOA) and a novel cumulative distribution plot were used to assess inter-session agreement.</p><p><strong>Results: </strong>Intersession dark-adapted a-wave showed a correlation of ρ = 0.73, (P < 0.0001), LOA: -106.4 to 113.9 µV, and 90% of measurements fell within - 24.8 to 58.0% variation (90% range). Intersession dark-adapted b-wave amplitudes showed ρ = 0.65, (P < 0.001), LOA: - 402.5 to 437.4 µV, and 90% range: - 33.7 to 72.6%. Inter-session PhNR-B (base-to-trough) and PhNR-P (peak-to-trough) amplitudes showed ρ = 0.66 and 0.72 respectively; LOA: - 26.7 to 31.1 µV and - 98.9 to 100.1 µV respectively; 90% ranges: - 58.2 to 59.3%, and - 45.6 to 60.9%, respectively. Inter-session VEP P2 amplitude showed ρ = 0.67, (P < 0.001), LOA: 78.5-72.8 µV, and 90% range: - 52.2 to 67.9%. Inter-session VEP implicit time showed ρ = 0.48, (P < 0.05), LOA: - 39.4 to 38.3 ms, and 90% range: - 39.2% to 16.7%.</p><p><strong>Conclusion: </strong>The study reported the inter-session repeatability of the ERG and VEP tests using the Celeris system. These data facilitate the study design and sample size calculation for future preclinical studies using the Celeris, and serve as a reference for future comparison with other systems, protocols and species.</p>","PeriodicalId":11207,"journal":{"name":"Documenta Ophthalmologica","volume":" ","pages":"133-143"},"PeriodicalIF":2.9000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436515/pdf/","citationCount":"0","resultStr":"{\"title\":\"Inter-session repeatability of electroretinography and visual evoked potentials of the Celeris system.\",\"authors\":\"Xiuying Tang, Carla J Abbott, Penelope J Allen, Chi D Luu\",\"doi\":\"10.1007/s10633-025-10045-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>The Celeris system (Diagnosys LLC) offers a streamlined alternative to the gold-standard Espion system for high-throughput electroretinography (ERG) and visual evoked potentials (VEP) in preclinical studies. This study evaluated its inter-session repeatability of ERG and VEP measurements in healthy rodent retinae.</p><p><strong>Methods: </strong>Twenty-five wild type Brown Norway rats underwent ERG and VEP testing across two sessions. Dark-adapted ERG a- and b-waves (flash stimulus of 10 cd s/m<sup>2</sup>), and the photopic negative response (PhNR) (flash stimulus of 3 cd s/m<sup>2</sup>) were recorded. VEPs (flash stimulus of 3 cd s/m<sup>2</sup>) were recorded from implanted screw electrodes in the skull. Pearson's correlation coefficient (ρ), Bland-Altman with 95% limits of agreement (LOA) and a novel cumulative distribution plot were used to assess inter-session agreement.</p><p><strong>Results: </strong>Intersession dark-adapted a-wave showed a correlation of ρ = 0.73, (P < 0.0001), LOA: -106.4 to 113.9 µV, and 90% of measurements fell within - 24.8 to 58.0% variation (90% range). Intersession dark-adapted b-wave amplitudes showed ρ = 0.65, (P < 0.001), LOA: - 402.5 to 437.4 µV, and 90% range: - 33.7 to 72.6%. Inter-session PhNR-B (base-to-trough) and PhNR-P (peak-to-trough) amplitudes showed ρ = 0.66 and 0.72 respectively; LOA: - 26.7 to 31.1 µV and - 98.9 to 100.1 µV respectively; 90% ranges: - 58.2 to 59.3%, and - 45.6 to 60.9%, respectively. Inter-session VEP P2 amplitude showed ρ = 0.67, (P < 0.001), LOA: 78.5-72.8 µV, and 90% range: - 52.2 to 67.9%. Inter-session VEP implicit time showed ρ = 0.48, (P < 0.05), LOA: - 39.4 to 38.3 ms, and 90% range: - 39.2% to 16.7%.</p><p><strong>Conclusion: </strong>The study reported the inter-session repeatability of the ERG and VEP tests using the Celeris system. 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引用次数: 0
摘要
目的：Celeris系统（diagnostics LLC）为临床前研究中的高通量视网膜电图（ERG）和视觉诱发电位（VEP）提供了一种精简的替代金标准Espion系统。本研究评估了其在健康啮齿动物视网膜中ERG和VEP测量的间歇可重复性。方法：对25只野生型褐威大鼠进行ERG和VEP测试。记录暗适应ERG a波和b波（10 cd s/m2的闪光刺激）和光负反应（3 cd s/m2的闪光刺激）。在颅骨内植入螺钉电极，记录vep （3 cd s/m2的闪光刺激）。使用Pearson相关系数（ρ）、Bland-Altman 95%一致性限（LOA）和一种新的累积分布图来评估会议间一致性。结果：会话间暗适应a波的相关系数为ρ = 0.73, (P)结论：本研究报道了使用Celeris系统进行ERG和VEP测试的会话间可重复性。这些数据为将来使用Celeris进行临床前研究的研究设计和样本量计算提供了便利，并可作为将来与其他系统、方案和物种进行比较的参考。
Inter-session repeatability of electroretinography and visual evoked potentials of the Celeris system.
Purpose: The Celeris system (Diagnosys LLC) offers a streamlined alternative to the gold-standard Espion system for high-throughput electroretinography (ERG) and visual evoked potentials (VEP) in preclinical studies. This study evaluated its inter-session repeatability of ERG and VEP measurements in healthy rodent retinae.
Methods: Twenty-five wild type Brown Norway rats underwent ERG and VEP testing across two sessions. Dark-adapted ERG a- and b-waves (flash stimulus of 10 cd s/m2), and the photopic negative response (PhNR) (flash stimulus of 3 cd s/m2) were recorded. VEPs (flash stimulus of 3 cd s/m2) were recorded from implanted screw electrodes in the skull. Pearson's correlation coefficient (ρ), Bland-Altman with 95% limits of agreement (LOA) and a novel cumulative distribution plot were used to assess inter-session agreement.
Results: Intersession dark-adapted a-wave showed a correlation of ρ = 0.73, (P < 0.0001), LOA: -106.4 to 113.9 µV, and 90% of measurements fell within - 24.8 to 58.0% variation (90% range). Intersession dark-adapted b-wave amplitudes showed ρ = 0.65, (P < 0.001), LOA: - 402.5 to 437.4 µV, and 90% range: - 33.7 to 72.6%. Inter-session PhNR-B (base-to-trough) and PhNR-P (peak-to-trough) amplitudes showed ρ = 0.66 and 0.72 respectively; LOA: - 26.7 to 31.1 µV and - 98.9 to 100.1 µV respectively; 90% ranges: - 58.2 to 59.3%, and - 45.6 to 60.9%, respectively. Inter-session VEP P2 amplitude showed ρ = 0.67, (P < 0.001), LOA: 78.5-72.8 µV, and 90% range: - 52.2 to 67.9%. Inter-session VEP implicit time showed ρ = 0.48, (P < 0.05), LOA: - 39.4 to 38.3 ms, and 90% range: - 39.2% to 16.7%.
Conclusion: The study reported the inter-session repeatability of the ERG and VEP tests using the Celeris system. These data facilitate the study design and sample size calculation for future preclinical studies using the Celeris, and serve as a reference for future comparison with other systems, protocols and species.
期刊介绍：
Documenta Ophthalmologica is an official publication of the International Society for Clinical Electrophysiology of Vision. The purpose of the journal is to promote the understanding and application of clinical electrophysiology of vision. Documenta Ophthalmologica will publish reviews, research articles, technical notes, brief reports and case studies which inform the readers about basic and clinical sciences related to visual electrodiagnosis and means to improve diagnosis and clinical management of patients using visual electrophysiology. Studies may involve animals or humans. In either case appropriate care must be taken to follow the Declaration of Helsinki for human subject or appropriate humane standards of animal care (e.g., the ARVO standards on Animal Care and Use).
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