核酸适体功能化磁性共价有机框架用于牛奶中庆大霉素的快速提取和LC-MS/MS定量

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-08-10 DOI:10.1016/j.foodchem.2025.145889

Hongmei Liu , Hao Cao , Yangjiayi Xiang , Cong Hu , Gou Wu , Yan Li

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引用次数: 0

摘要

由于在兽药中的广泛使用，食品中的庆大霉素（GEN）残留已经促使了严格的法规和对简单、快速检测方法的需求。本文合成了aptamer修饰的磁性共价有机骨架（COFs） TpDBD@Fe₃O₄@apt，并将其作为吸附剂应用于磁性固相萃取（MSPE）中，用于牛奶中GEN残留物的高特异性捕获和富集。对影响MSPE效率的几个因素进行了系统评价和优化。结合液相色谱-串联质谱（LC-MS/MS），该方法线性范围内良好（R2 ≥ 0.999），检出限低（0.48 ~ 0.54 ng/mL），加样回收率高（92.7 % ~ 108.8 %），精密度高（rsd≤10.8 %）。该方法成功地应用于实际牛奶样品中，为GEN残留的检测提供了一种有效的解决方案。

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Aptamer-functionalized magnetic covalent organic frameworks for rapid extraction and LC-MS/MS quantification of gentamicin in milk

Gentamicin (GEN) residues in food products, arising from its extensive use in veterinary medicine, have prompted stringent regulations and a demand for simple, rapid detection methods. In this study, aptamer-modified magnetic covalent organic frameworks (COFs), denoted as TpDBD@Fe₃O₄@apt, were synthesized and employed as adsorbents in magnetic solid-phase extraction (MSPE) for the highly specific capture and enrichment of GEN residues in milk. Several factors influencing MSPE efficiency were systematically evaluated and optimized. Coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS), the developed method exhibited good linearity within the linear range (R² ≥ 0.999), low detection limits (0.48–0.54 ng/mL), satisfactory recoveries (92.7 %–108.8 %), and high precision (RSDs ≤10.8 %). The proposed method was successfully applied to real milk samples, providing an efficient solution for the detection of GEN residues.

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.