通过调整羧基-氨基间距设计具有超亲水性的两性离子肽,用于复杂生物流体中稳健的防污电化学生物传感。

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Zhen Song, Rui Han, Kunpeng Yu and Xiliang Luo*, 
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引用次数: 0

摘要

电化学生物传感器的性能受到复杂生物流体中生物污垢的严重影响,因此需要探索高性能防污材料来构建坚固的无污垢电化学生物传感器。本研究通过调节两性离子多肽相邻氨基和羧基之间的距离,设计了防污型两性离子多肽CPPPP(D-Dap)(D-Dap)(D-Dap)(D-Dap) (CP(DDap)),并将其用于制备防污型电化学生物传感器。与传统两性离子肽相比,CP(DDap)不仅具有超亲水性,而且具有更高的结构刚性和更小的偶极矩,使其在复杂的生物介质中具有优异的防污性能,同时其非自然特性使其在生物流体中具有抗天然蛋白酶水解的稳定性。基于CP(DDap)的生物传感器对血清中皮质醇的检测具有较高的灵敏度,检测限极低,为3.5 pg·mL-1,并且在3周的真实血清中显示出优异的防污性能。值得注意的是,该生物传感器在一系列临床样品中获得的检测结果与使用商业皮质醇检测试剂盒获得的结果一致。本研究探索的两性离子多肽中调节氨基羧基间距的策略为设计高效防污材料提供了一种新方法,从而推动了面向复杂生物流体实际应用的鲁棒生物传感器和生物电子学的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Designing Zwitterionic Peptide with Superhydrophilicity through Carboxyl–Amino Spacing Adjustment for Robust Antifouling Electrochemical Biosensing in Complex Biofluids

Designing Zwitterionic Peptide with Superhydrophilicity through Carboxyl–Amino Spacing Adjustment for Robust Antifouling Electrochemical Biosensing in Complex Biofluids

The performance of electrochemical biosensors is severely compromised by biofouling in complex biofluids, underscoring the necessity to explore high-performance antifouling materials to construct robust nonfouling electrochemical biosensors. Here, the antifouling zwitterionic peptide CPPPP(D-Dap)(D-Dap)(D-Dap)(D-Dap) (CP(DDap)) was designed by adjusting the distance between the adjacent amino and carboxyl groups of the peptide, and it was further employed to fabricate antifouling electrochemical biosensors. Compared with traditional zwitterionic peptides, CP(DDap) exhibited not only superhydrophilicity but also higher structural rigidity and a smaller dipole moment, enabling it to achieve superior antifouling performance in complex biological media, while its unnatural characteristics endowed it with robust stability against hydrolysis by natural proteinase in biofluids. The CP(DDap)-based biosensor demonstrated high sensitivity for cortisol detection in serum with a remarkably low detection limit of 3.5 pg·mL–1, and it displayed superior antifouling performance in real serum over 3 weeks. Notably, the assay results obtained by this biosensor in a series of clinical samples were consistent with those obtained using a commercial cortisol assay kit. The strategy of adjusting amino–carboxyl spacing in zwitterionic peptides explored in this study provides a new approach for designing highly efficient antifouling materials, thereby advancing the development of robust biosensors and bioelectronics tailored for practical applications in complex biofluids.

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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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