{"title":"纳米液滴界面电离-质谱联用高灵敏度分析多环芳烃。","authors":"Zehu Xie, Siyuan Tan, Lulu Feng, Simin Cheng, Xiang Fang, Xinbin Cheng*, Xiaoyun Gong* and Xinhua Dai*, ","doi":"10.1021/acs.analchem.5c03362","DOIUrl":null,"url":null,"abstract":"<p >Polycyclic aromatic hydrocarbons (PAHs) are well-known environmental pollutants with significant carcinogenic potential. The detection of PAHs by mass spectrometry (MS) remains challenging due to their trace contents and extremely low ionization efficiency. The emerging microdroplet-based MS techniques utilize interfacial reactions for the acceleration of chemical reactions and improvement of ionization efficiency, thereby offering high selectivity and sensitivity for complex samples. In this study, we present a novel nanodroplet interfacial ionization (NII) technique that achieved direct detection of PAHs in complex biological samples with high sensitivity at attomole (amol) level. It significantly increased the ionization efficiency of PAHs by converting them into radical cations (M<sup>•+</sup>) through interfacial reactions at the surfaces of nanodroplets. The effectiveness of NII for the ionization of PAHs was confirmed by the analysis of a wide range of PAHs and their derivatives. The practicality of NII was further demonstrated by its application in the analysis of trace PAHs and related metabolites in single mammalian cells. NII holds great potential in environmental studies on the toxicological effects of aromatic pollutants. Furthermore, it might also be beneficial to research studies in the activation of aromatic rings for chemical synthesis.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 32","pages":"17795–17803"},"PeriodicalIF":6.7000,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"High-Sensitivity Analysis of Polycyclic Aromatic Hydrocarbons by Nanodroplet Interfacial Ionization Coupled with Mass Spectrometry\",\"authors\":\"Zehu Xie, Siyuan Tan, Lulu Feng, Simin Cheng, Xiang Fang, Xinbin Cheng*, Xiaoyun Gong* and Xinhua Dai*, \",\"doi\":\"10.1021/acs.analchem.5c03362\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Polycyclic aromatic hydrocarbons (PAHs) are well-known environmental pollutants with significant carcinogenic potential. The detection of PAHs by mass spectrometry (MS) remains challenging due to their trace contents and extremely low ionization efficiency. The emerging microdroplet-based MS techniques utilize interfacial reactions for the acceleration of chemical reactions and improvement of ionization efficiency, thereby offering high selectivity and sensitivity for complex samples. In this study, we present a novel nanodroplet interfacial ionization (NII) technique that achieved direct detection of PAHs in complex biological samples with high sensitivity at attomole (amol) level. It significantly increased the ionization efficiency of PAHs by converting them into radical cations (M<sup>•+</sup>) through interfacial reactions at the surfaces of nanodroplets. The effectiveness of NII for the ionization of PAHs was confirmed by the analysis of a wide range of PAHs and their derivatives. The practicality of NII was further demonstrated by its application in the analysis of trace PAHs and related metabolites in single mammalian cells. NII holds great potential in environmental studies on the toxicological effects of aromatic pollutants. Furthermore, it might also be beneficial to research studies in the activation of aromatic rings for chemical synthesis.</p>\",\"PeriodicalId\":27,\"journal\":{\"name\":\"Analytical Chemistry\",\"volume\":\"97 32\",\"pages\":\"17795–17803\"},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2025-08-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.analchem.5c03362\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.analchem.5c03362","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
High-Sensitivity Analysis of Polycyclic Aromatic Hydrocarbons by Nanodroplet Interfacial Ionization Coupled with Mass Spectrometry
Polycyclic aromatic hydrocarbons (PAHs) are well-known environmental pollutants with significant carcinogenic potential. The detection of PAHs by mass spectrometry (MS) remains challenging due to their trace contents and extremely low ionization efficiency. The emerging microdroplet-based MS techniques utilize interfacial reactions for the acceleration of chemical reactions and improvement of ionization efficiency, thereby offering high selectivity and sensitivity for complex samples. In this study, we present a novel nanodroplet interfacial ionization (NII) technique that achieved direct detection of PAHs in complex biological samples with high sensitivity at attomole (amol) level. It significantly increased the ionization efficiency of PAHs by converting them into radical cations (M•+) through interfacial reactions at the surfaces of nanodroplets. The effectiveness of NII for the ionization of PAHs was confirmed by the analysis of a wide range of PAHs and their derivatives. The practicality of NII was further demonstrated by its application in the analysis of trace PAHs and related metabolites in single mammalian cells. NII holds great potential in environmental studies on the toxicological effects of aromatic pollutants. Furthermore, it might also be beneficial to research studies in the activation of aromatic rings for chemical synthesis.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.