纳米液滴界面电离-质谱联用高灵敏度分析多环芳烃。

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Zehu Xie, Siyuan Tan, Lulu Feng, Simin Cheng, Xiang Fang, Xinbin Cheng*, Xiaoyun Gong* and Xinhua Dai*, 
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引用次数: 0

摘要

多环芳烃(PAHs)是众所周知的具有显著致癌潜力的环境污染物。由于多环芳烃的痕量和极低的电离效率,用质谱法检测多环芳烃仍然是一个挑战。新兴的基于微滴的质谱技术利用界面反应来加速化学反应和提高电离效率,从而为复杂样品提供高选择性和灵敏度。在这项研究中,我们提出了一种新的纳米液滴界面电离(NII)技术,以高灵敏度在原子孔(amol)水平上直接检测复杂生物样品中的多环芳烃。它通过纳米液滴表面的界面反应将多环芳烃转化为自由基阳离子(M•+),显著提高了多环芳烃的电离效率。通过对多种多环芳烃及其衍生物的分析,证实了NII对多环芳烃电离的有效性。NII在哺乳动物单个细胞中痕量多环芳烃及相关代谢物分析中的应用进一步证明了其实用性。NII在芳香污染物毒理学效应的环境研究中具有很大的潜力。此外,它也可能有利于研究芳香环的活化用于化学合成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

High-Sensitivity Analysis of Polycyclic Aromatic Hydrocarbons by Nanodroplet Interfacial Ionization Coupled with Mass Spectrometry

High-Sensitivity Analysis of Polycyclic Aromatic Hydrocarbons by Nanodroplet Interfacial Ionization Coupled with Mass Spectrometry

Polycyclic aromatic hydrocarbons (PAHs) are well-known environmental pollutants with significant carcinogenic potential. The detection of PAHs by mass spectrometry (MS) remains challenging due to their trace contents and extremely low ionization efficiency. The emerging microdroplet-based MS techniques utilize interfacial reactions for the acceleration of chemical reactions and improvement of ionization efficiency, thereby offering high selectivity and sensitivity for complex samples. In this study, we present a novel nanodroplet interfacial ionization (NII) technique that achieved direct detection of PAHs in complex biological samples with high sensitivity at attomole (amol) level. It significantly increased the ionization efficiency of PAHs by converting them into radical cations (M•+) through interfacial reactions at the surfaces of nanodroplets. The effectiveness of NII for the ionization of PAHs was confirmed by the analysis of a wide range of PAHs and their derivatives. The practicality of NII was further demonstrated by its application in the analysis of trace PAHs and related metabolites in single mammalian cells. NII holds great potential in environmental studies on the toxicological effects of aromatic pollutants. Furthermore, it might also be beneficial to research studies in the activation of aromatic rings for chemical synthesis.

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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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