5- ala诱导的PPIX积累对神经干细胞行为的影响。

IF 3.7 3区医学 Q2 NEUROSCIENCES

Brain Research Bulletin Pub Date : 2025-07-24 DOI:10.1016/j.brainresbull.2025.111480

Elham Poonaki , Sedra Badlah , Ulf Dietrich Kahlert , Sven G. Meuth , Walter Stummer , Ali Gorji

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引用次数: 0

摘要

5-氨基乙酰丙酸（5-ALA）是一种在手术干预中被广泛认可的改善肿瘤切除的有效工具，但可能直接与肿瘤微环境中的细胞相互作用。然而，关于5-ALA对神经干细胞（NSCs）的影响仍存在争议。本研究旨在探讨5-ALA对NSCs和少突胶质细胞祖细胞（OPCs）的影响。本研究从大鼠脑室下区分离NSCs并分化为OPCs。随后用5-ALA处理NSCs和OPCs，并通过免疫染色和集落形成试验评估其效果。我们的研究结果表明，5-ALA处理诱导PPIX在NSCs和OPCs中积累，NSCs的PPIX水平较高，可能是由于它们的增殖速度更快。此外，我们的研究结果表明，PPIX的长期积累会损害NSC的克隆原性。这些结果强调了5- ala诱导的PPIX可能与NSCs的相互作用。5-ALA有望成为NSCs的潜在标记物，但也可能通过光或放疗激活卟啉来特异性靶向NSCs。

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Impact of 5-ALA-induced PPIX accumulation on neural stem cell behavior

5-aminolevulinic acid (5-ALA) is a widely recognized and effective tool for improving tumor resections during surgical interventions but may directly interact with cells in the tumor microenvironment. Nevertheless, there remains an ongoing debate regarding the impact of 5-ALA on neural stem cells (NSCs). This study aims to investigate the effects of 5-ALA on both NSCs and oligodendrocyte progenitor cells (OPCs). In this study, NSCs were isolated from the subventricular zones of rat brains and differentiated into OPCs. Both NSCs and OPCs were subsequently treated with 5-ALA, and their effects were evaluated through immunostaining and colony-formation assays. Our findings show that 5-ALA treatment induces PPIX accumulation in both NSCs and OPCs, with NSCs exhibiting higher levels presumably due to their greater proliferation rate. Furthermore, our results indicate that prolonged PPIX accumulation impairs NSC clonogenicity. These results underscore possible interactions of 5-ALA-induced PPIX with NSCs. 5-ALA shows promise as a potential marker for NSCs, but may also be of value for specifically targeting NSCs through activation of porphyrins using light or radiotherapy.

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来源期刊

Brain Research Bulletin 医学-神经科学

CiteScore

6.90

自引率

2.60%

发文量

253

审稿时长

67 days

期刊介绍： The Brain Research Bulletin (BRB) aims to publish novel work that advances our knowledge of molecular and cellular mechanisms that underlie neural network properties associated with behavior, cognition and other brain functions during neurodevelopment and in the adult. Although clinical research is out of the Journal''s scope, the BRB also aims to publish translation research that provides insight into biological mechanisms and processes associated with neurodegeneration mechanisms, neurological diseases and neuropsychiatric disorders. The Journal is especially interested in research using novel methodologies, such as optogenetics, multielectrode array recordings and life imaging in wild-type and genetically-modified animal models, with the goal to advance our understanding of how neurons, glia and networks function in vivo.