超光稳定荧光硼桥近红外探针在STED超分辨率下揭示脂滴-线粒体相互作用的新模式

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-07-22 DOI:10.1021/acs.analchem.5c02739

Mingyue Cao,Xiaoming Zhu,Yunting Liu,Yangang Su,Xinru Hu,Zhiqiang Liu,Xiaoqiang Yu

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引用次数: 0

摘要

脂滴与线粒体之间的相互作用对于维持细胞代谢和能量稳态至关重要。然而，在活细胞中以高分辨率可视化这些相互作用动力学仍然是非常具有挑战性的。本研究开发了一种硼桥近红外探头（QI-BF），专门用于ld的低功率受激发射耗尽（STED）超分辨率成像。QI-BF表现出优异的光稳定性，可以使用STED激光（0.96 MW cm- 2,775 nm）连续扫描1000+帧，没有明显的荧光衰减。此外，在商用线粒体染料的帮助下，实现了ld与线粒体之间形态相互作用的动态双色STED超分辨率可视化。首次成功捕获了线粒体包裹在ld周围和ld介导的线粒体管化的动态过程。该研究为开发用于多功能低功率STED纳米显微镜的超热稳定有机荧光探针提供了思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Ultra-Photostable Fluorescent Boron-Bridged Near-Infrared Probes to Reveal New Mode of Lipid Droplet-Mitochondria Interaction at STED Super-Resolution.

Interactions between lipid droplets (LDs) and mitochondria are vital for maintaining the cellular metabolism and energy homeostasis. However, visualizing these interaction dynamics at a high resolution in live cells is still very challenging. Here, a boron-bridged near-infrared probe (QI-BF) was developed specifically for low-power stimulated emission depletion (STED) super-resolution imaging of LDs. QI-BF exhibited excellent photostability and enabled continuous scanning of 1000+ frames using a STED laser (0.96 MW cm-2, 775 nm), without significant fluorescence decay. Furthermore, dynamic dual-color STED super-resolution visualization of the morphological interactions between LDs and mitochondria was achieved with the aid of commercial mitochondrial dyes. For the first time, the dynamic process of mitochondrial wrapping around the LDs and LD-mediated mitochondria tubulation were successfully captured. This study sheds light on developing ultraphotostable organic fluorescent probes for versatile low-power STED nanoscopy.

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.