耐环丙沙星铜绿假单胞菌对高浓度含环丙沙星耳外用溶液的体外敏感性研究。

IF 2.2 4区医学 Q2 INFECTIOUS DISEASES

Infectious diseases now Pub Date : 2025-07-18 DOI:10.1016/j.idnow.2025.105120

Tali Jane Krotter , Hagai Rechnitzer , Ohad Ronen

{"title":"耐环丙沙星铜绿假单胞菌对高浓度含环丙沙星耳外用溶液的体外敏感性研究。","authors":"Tali Jane Krotter , Hagai Rechnitzer , Ohad Ronen","doi":"10.1016/j.idnow.2025.105120","DOIUrl":null,"url":null,"abstract":"<div><h3>Context</h3><div><em>Pseudomonas aeruginosa</em> is the most frequently isolated pathogen in acute otitis externa (AOE), with ototopical antibiotics being the mainstay of treatment. The incidence of ciprofloxacin resistance in <em>P.aeruginosa</em> has been steadily increasing. This study aimed to evaluate the in vitro susceptibility of <em>P.aeruginosa</em> to a ciprofloxacin-containing ototopical solution.</div></div><div><h3>Methods</h3><div>In this prospective study we examined the in vitro growth of ciprofloxacin-resistant <em>P.aeruginosa</em> isolates when exposed to varying concentrations of a ciprofloxacin-containing ototopical solution.</div></div><div><h3>Results</h3><div>Concentration of the ototopical solution was strongly associated with the diameter of inhibition on sensitive strains. Sensitive strains of <em>P.aeruginosa</em> showed inhibition of growth with an extrapolated MIC value of 0.0686 µg/µL. Resistant strains demonstrated inhibition to all dilutions of the ototopical solution, including undiluted concentration. Ciprofloxacin-sensitive strains demonstrated resistance at higher ototopical concentrations compared with the reported serum concentration after standard systemic administration.</div></div><div><h3>Conclusions</h3><div>The current use of commercially available ciprofloxacin topical ear drops for treating AOE does not appear to hold promise for the fight against resistant strains of <em>P.aeruginosa</em> and might be at risk of becoming ineffective in the treatment of sensitive species. Therefore, further investigation is needed to gain a deeper understanding of ototopical solutions, as well as to establish a database of MICs for topical antibiotics.</div></div>","PeriodicalId":13539,"journal":{"name":"Infectious diseases now","volume":"55 7","pages":"Article 105120"},"PeriodicalIF":2.2000,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In vitro susceptibility of ciprofloxacin-resistant strains of Pseudomonas aeruginosa to high concentration ciprofloxacin-containing ototopical solution\",\"authors\":\"Tali Jane Krotter , Hagai Rechnitzer , Ohad Ronen\",\"doi\":\"10.1016/j.idnow.2025.105120\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Context</h3><div><em>Pseudomonas aeruginosa</em> is the most frequently isolated pathogen in acute otitis externa (AOE), with ototopical antibiotics being the mainstay of treatment. The incidence of ciprofloxacin resistance in <em>P.aeruginosa</em> has been steadily increasing. This study aimed to evaluate the in vitro susceptibility of <em>P.aeruginosa</em> to a ciprofloxacin-containing ototopical solution.</div></div><div><h3>Methods</h3><div>In this prospective study we examined the in vitro growth of ciprofloxacin-resistant <em>P.aeruginosa</em> isolates when exposed to varying concentrations of a ciprofloxacin-containing ototopical solution.</div></div><div><h3>Results</h3><div>Concentration of the ototopical solution was strongly associated with the diameter of inhibition on sensitive strains. Sensitive strains of <em>P.aeruginosa</em> showed inhibition of growth with an extrapolated MIC value of 0.0686 µg/µL. Resistant strains demonstrated inhibition to all dilutions of the ototopical solution, including undiluted concentration. Ciprofloxacin-sensitive strains demonstrated resistance at higher ototopical concentrations compared with the reported serum concentration after standard systemic administration.</div></div><div><h3>Conclusions</h3><div>The current use of commercially available ciprofloxacin topical ear drops for treating AOE does not appear to hold promise for the fight against resistant strains of <em>P.aeruginosa</em> and might be at risk of becoming ineffective in the treatment of sensitive species. Therefore, further investigation is needed to gain a deeper understanding of ototopical solutions, as well as to establish a database of MICs for topical antibiotics.</div></div>\",\"PeriodicalId\":13539,\"journal\":{\"name\":\"Infectious diseases now\",\"volume\":\"55 7\",\"pages\":\"Article 105120\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-07-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infectious diseases now\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666991925000995\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infectious diseases now","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666991925000995","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

摘要

背景：铜绿假单胞菌是急性外源性中耳炎（AOE）中最常见的分离病原体，耳外用抗生素是主要的治疗方法。铜绿假单胞菌耐环丙沙星的发生率呈稳步上升趋势。本研究旨在评价铜绿假单胞菌对含环丙沙星耳外用溶液的体外敏感性。方法：在这项前瞻性研究中，我们检测了暴露于不同浓度的含环丙沙星耳外用溶液中耐环丙沙星铜绿假单胞菌的体外生长。结果：耳表面溶液的浓度与对敏感菌的抑制直径密切相关。P.aeruginosa敏感菌株对生长有抑制作用，外推MIC值为0.0686 µg/µL。耐药菌株表现出抑制所有稀释的耳表面溶液，包括未稀释的浓度。环丙沙星敏感菌株在较高的耳表面浓度下表现出耐药性，与标准全身给药后报告的血清浓度相比。结论：目前市面上可买到的环丙沙星局部滴耳液用于治疗AOE似乎对铜绿假单胞菌耐药菌株没有希望，并且可能有在治疗敏感菌株时无效的风险。因此，需要进一步研究以加深对耳外用溶液的了解，并建立外用抗生素mic数据库。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

In vitro susceptibility of ciprofloxacin-resistant strains of Pseudomonas aeruginosa to high concentration ciprofloxacin-containing ototopical solution

Context

Pseudomonas aeruginosa is the most frequently isolated pathogen in acute otitis externa (AOE), with ototopical antibiotics being the mainstay of treatment. The incidence of ciprofloxacin resistance in P. aeruginosa has been steadily increasing. This study aimed to evaluate the in vitro susceptibility of P. aeruginosa to a ciprofloxacin-containing ototopical solution.

Methods

In this prospective study we examined the in vitro growth of ciprofloxacin-resistant P. aeruginosa isolates when exposed to varying concentrations of a ciprofloxacin-containing ototopical solution.

Results

Concentration of the ototopical solution was strongly associated with the diameter of inhibition on sensitive strains. Sensitive strains of P. aeruginosa showed inhibition of growth with an extrapolated MIC value of 0.0686 µg/µL. Resistant strains demonstrated inhibition to all dilutions of the ototopical solution, including undiluted concentration. Ciprofloxacin-sensitive strains demonstrated resistance at higher ototopical concentrations compared with the reported serum concentration after standard systemic administration.

Conclusions

The current use of commercially available ciprofloxacin topical ear drops for treating AOE does not appear to hold promise for the fight against resistant strains of P. aeruginosa and might be at risk of becoming ineffective in the treatment of sensitive species. Therefore, further investigation is needed to gain a deeper understanding of ototopical solutions, as well as to establish a database of MICs for topical antibiotics.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊