Peiyi Jin, Liping Wang, Jiaju Pi, Tiefeng Yuan, Jinjian Zhong, Linzhou Chen, Jilong Huang, Lin Gan, Chen Lin, Huajun Fan
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Microwave-assisted deep eutectic solvent extraction and macroporous resin purification of anthocyanins from Purple-heart Radish: Process optimization, structural characterization and quantitative analysis.
A novel approach for extraction and purification of the anthocyanins from Purple-heart Radish was developed by microwave-assisted deep eutectic solvent extraction (MAE-DES) coupled to macroporous resin separation (MRS). Citric acid-based DES as the extractant was screened and characterized from 13 synthesized deep eutectic solvents due to the excellent performance. Through RSM optimization of MAE-DES process, the anthocyanin yield reached 6.442 ± 0.135 mg/g, demonstrating that MAE-DES significantly superior to that of conventional methods. With AB-8 MRS purification, the anthocyanin purity was increased by 63-fold. By UHPLC-Q-TOF-MS/MS, the anthocyanins obtained from Purple-heart Radish were dominantly composed of pelargonins. Based on composition analysis, pH differential method was first modified by introducing weighting factors, avoiding serious underestimation of the anthocyanins content in Purple-heart Radish. MAE-DES and the modified spectrophotometry were successfully applied for assessment of anthocyanin extraction and sample drying methods, providing an efficient alternative to development and application of the anthocyanins from Purple-heart Radish.
期刊介绍：
Food Chemistry: X, one of three Open Access companion journals to Food Chemistry, follows the same aims, scope, and peer-review process. It focuses on papers advancing food and biochemistry or analytical methods, prioritizing research novelty. Manuscript evaluation considers novelty, scientific rigor, field advancement, and reader interest. Excluded are studies on food molecular sciences or disease cure/prevention. Topics include food component chemistry, bioactives, processing effects, additives, contaminants, and analytical methods. The journal welcome Analytical Papers addressing food microbiology, sensory aspects, and more, emphasizing new methods with robust validation and applicability to diverse foods or regions.
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