{"title":"低致敏性scyp1衍生物免疫诱导阻断抗体可减轻泥蟹过敏症状。","authors":"Meng-Si Li, Fei Xia, Qing-Mei Liu, Jun-Feng Zheng, Xin Guo, Li-Qiang Zhong, Gui-Xia Chen, Lian-Zhong Luo, Yi-Xiang Liu and Guang-Ming Liu*, ","doi":"10.1021/acs.jafc.5c05810","DOIUrl":null,"url":null,"abstract":"<p >Crab triggers severe IgE-mediated allergic reactions. A rabbit anti-IgG antibody induced by hypoallergenic derivatives of Scy p 1 (mtScy p 1) effectively blocked wild-type Scy p 1 (wtScy p 1) binding to serum IgE; however, its protection against allergic symptoms needs to be further studied. In this paper, rabbit anti-mtScy p 1 IgG antibody could inhibit wtScy p 1-induced basophil degranulation, mainly due to the overlap of 2 IgG conformational epitopes and 4 IgG linear epitopes with IgE epitopes. In Balb/c mice, compared with the wtScy p 1 group, the levels of IgE and IgG1, B cells, IL-4, and IL-17A were significantly decreased, whereas the production of IgG2a, TCR-β cells, Foxp3 cells, IFN-γ, and IL-10 was obviously increased in the rabbit anti-mtScy p 1 IgG antibody group, thereby alleviating the allergic response by modulating Th1/Th2 and Th17/Treg balance. These findings indicated that rabbit anti-mtScy p 1 IgG antibody holds promise for an immunotherapeutic regimen.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"73 30","pages":"19026–19035"},"PeriodicalIF":6.2000,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Blocking Antibody Induced by Immunization with Hypoallergenic Derivatives of Scy p 1 Alleviated Mud Crab Allergy Symptoms\",\"authors\":\"Meng-Si Li, Fei Xia, Qing-Mei Liu, Jun-Feng Zheng, Xin Guo, Li-Qiang Zhong, Gui-Xia Chen, Lian-Zhong Luo, Yi-Xiang Liu and Guang-Ming Liu*, \",\"doi\":\"10.1021/acs.jafc.5c05810\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Crab triggers severe IgE-mediated allergic reactions. A rabbit anti-IgG antibody induced by hypoallergenic derivatives of Scy p 1 (mtScy p 1) effectively blocked wild-type Scy p 1 (wtScy p 1) binding to serum IgE; however, its protection against allergic symptoms needs to be further studied. In this paper, rabbit anti-mtScy p 1 IgG antibody could inhibit wtScy p 1-induced basophil degranulation, mainly due to the overlap of 2 IgG conformational epitopes and 4 IgG linear epitopes with IgE epitopes. In Balb/c mice, compared with the wtScy p 1 group, the levels of IgE and IgG1, B cells, IL-4, and IL-17A were significantly decreased, whereas the production of IgG2a, TCR-β cells, Foxp3 cells, IFN-γ, and IL-10 was obviously increased in the rabbit anti-mtScy p 1 IgG antibody group, thereby alleviating the allergic response by modulating Th1/Th2 and Th17/Treg balance. These findings indicated that rabbit anti-mtScy p 1 IgG antibody holds promise for an immunotherapeutic regimen.</p>\",\"PeriodicalId\":41,\"journal\":{\"name\":\"Journal of Agricultural and Food Chemistry\",\"volume\":\"73 30\",\"pages\":\"19026–19035\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2025-07-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Agricultural and Food Chemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.jafc.5c05810\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Agricultural and Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jafc.5c05810","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
螃蟹会引发严重的ige介导的过敏反应。低致敏性scyp1衍生物（mtscyp1）诱导兔抗igg抗体有效阻断野生型scyp1 （wtscyp1）与血清IgE的结合；然而，其对过敏症状的保护作用有待进一步研究。本研究中，兔抗mtscyp1 IgG抗体能够抑制wtscyp1诱导的嗜碱性粒细胞脱粒，主要是由于2个IgG构象表位和4个IgG线性表位与IgE表位重叠。在Balb/c小鼠中，与wtScy p 1组相比，抗mtscy p 1 IgG组的IgE、IgG1、B细胞、IL-4、IL-17A水平显著降低，而兔抗mtscy p 1 IgG组的IgG2a、TCR-β细胞、Foxp3细胞、IFN-γ和IL-10的产生明显增加，从而通过调节Th1/Th2和Th17/Treg平衡来减轻过敏反应。这些发现表明兔抗mtscy p1igg抗体有望用于免疫治疗方案。
Blocking Antibody Induced by Immunization with Hypoallergenic Derivatives of Scy p 1 Alleviated Mud Crab Allergy Symptoms
Crab triggers severe IgE-mediated allergic reactions. A rabbit anti-IgG antibody induced by hypoallergenic derivatives of Scy p 1 (mtScy p 1) effectively blocked wild-type Scy p 1 (wtScy p 1) binding to serum IgE; however, its protection against allergic symptoms needs to be further studied. In this paper, rabbit anti-mtScy p 1 IgG antibody could inhibit wtScy p 1-induced basophil degranulation, mainly due to the overlap of 2 IgG conformational epitopes and 4 IgG linear epitopes with IgE epitopes. In Balb/c mice, compared with the wtScy p 1 group, the levels of IgE and IgG1, B cells, IL-4, and IL-17A were significantly decreased, whereas the production of IgG2a, TCR-β cells, Foxp3 cells, IFN-γ, and IL-10 was obviously increased in the rabbit anti-mtScy p 1 IgG antibody group, thereby alleviating the allergic response by modulating Th1/Th2 and Th17/Treg balance. These findings indicated that rabbit anti-mtScy p 1 IgG antibody holds promise for an immunotherapeutic regimen.
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The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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