{"title":"季节和地理变异、反相高效液相色谱- pda定量分析及锦葵咔唑类生物碱的抗肥胖潜力Spreng。","authors":"Mridula Singh, Akash Dey, Dixita Rameshbhai Dhoriya, Amit Kumar Srivastava, Alok Goyal, Kirti Nandkumar Deshmukh, Sanjay Madhukar Jachak","doi":"10.1002/pca.70010","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Murraya koenigii contains bioactive compounds with antidiabetic and hypolipidemic activities. Considering widespread availability and traditional use of M. koenigii throughout South Asia.</p><p><strong>Objectives: </strong>The present study was designed to perform qualitative and quantitative analysis of phytochemicals present in curry leaves.</p><p><strong>Material and methods: </strong>Quantification method was validated according to International Council of Harmonization (ICH) guidelines and was used to analyze seasonal and geographical variability of phytoconstituents in leaf samples collected from different geographical locations across India.</p><p><strong>Results: </strong>The isolated four carbazole alkaloids, namely, mahanimbine, girinimbine, koenimbine, and koenigicine, from methanolic extract of curry leaves, were quantitatively determined in the leaves of M. koenigii using reverse phase-high performance liquid chromatography equipped with photodiode array detector (RP-HPLC-PDA).</p><p><strong>Conclusions: </strong>Our study revealed that mahanimbine, koenimbine, and koenigicine content was maximum in sample collected in months of April, June, and October 2020, respectively. The samples procured from Northern Indian regions showed maximum content of carbazole alkaloids. The in vitro biological assays revealed anti-obesogenic potential of carbazole alkaloids in 3T3-L1 adipocytes.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"2039-2049"},"PeriodicalIF":2.6000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Seasonal and Geographical Variability, Quantitative Analysis by RP-HPLC-PDA, and Anti-Obesogenic Potential of Carbazole Alkaloids of Murraya koenigii (L.) Spreng.\",\"authors\":\"Mridula Singh, Akash Dey, Dixita Rameshbhai Dhoriya, Amit Kumar Srivastava, Alok Goyal, Kirti Nandkumar Deshmukh, Sanjay Madhukar Jachak\",\"doi\":\"10.1002/pca.70010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Murraya koenigii contains bioactive compounds with antidiabetic and hypolipidemic activities. Considering widespread availability and traditional use of M. koenigii throughout South Asia.</p><p><strong>Objectives: </strong>The present study was designed to perform qualitative and quantitative analysis of phytochemicals present in curry leaves.</p><p><strong>Material and methods: </strong>Quantification method was validated according to International Council of Harmonization (ICH) guidelines and was used to analyze seasonal and geographical variability of phytoconstituents in leaf samples collected from different geographical locations across India.</p><p><strong>Results: </strong>The isolated four carbazole alkaloids, namely, mahanimbine, girinimbine, koenimbine, and koenigicine, from methanolic extract of curry leaves, were quantitatively determined in the leaves of M. koenigii using reverse phase-high performance liquid chromatography equipped with photodiode array detector (RP-HPLC-PDA).</p><p><strong>Conclusions: </strong>Our study revealed that mahanimbine, koenimbine, and koenigicine content was maximum in sample collected in months of April, June, and October 2020, respectively. The samples procured from Northern Indian regions showed maximum content of carbazole alkaloids. The in vitro biological assays revealed anti-obesogenic potential of carbazole alkaloids in 3T3-L1 adipocytes.</p>\",\"PeriodicalId\":20095,\"journal\":{\"name\":\"Phytochemical Analysis\",\"volume\":\" \",\"pages\":\"2039-2049\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytochemical Analysis\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1002/pca.70010\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/7/13 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytochemical Analysis","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/pca.70010","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/13 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Seasonal and Geographical Variability, Quantitative Analysis by RP-HPLC-PDA, and Anti-Obesogenic Potential of Carbazole Alkaloids of Murraya koenigii (L.) Spreng.
Introduction: Murraya koenigii contains bioactive compounds with antidiabetic and hypolipidemic activities. Considering widespread availability and traditional use of M. koenigii throughout South Asia.
Objectives: The present study was designed to perform qualitative and quantitative analysis of phytochemicals present in curry leaves.
Material and methods: Quantification method was validated according to International Council of Harmonization (ICH) guidelines and was used to analyze seasonal and geographical variability of phytoconstituents in leaf samples collected from different geographical locations across India.
Results: The isolated four carbazole alkaloids, namely, mahanimbine, girinimbine, koenimbine, and koenigicine, from methanolic extract of curry leaves, were quantitatively determined in the leaves of M. koenigii using reverse phase-high performance liquid chromatography equipped with photodiode array detector (RP-HPLC-PDA).
Conclusions: Our study revealed that mahanimbine, koenimbine, and koenigicine content was maximum in sample collected in months of April, June, and October 2020, respectively. The samples procured from Northern Indian regions showed maximum content of carbazole alkaloids. The in vitro biological assays revealed anti-obesogenic potential of carbazole alkaloids in 3T3-L1 adipocytes.
期刊介绍：
Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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