模拟磷酸酶和漆酶级联催化超灵敏比色法检测丙烯诺福的构建

IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED
Miao Chen, Xuerong Li, Qiulan Li, Jitao Li, Yaohong Gu, Peng Yang, Bo Yang, Yaling Yang
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引用次数: 0

摘要

基于纳米酶的去磷酸化反应显示出监测有机磷农药(OPs)的潜力,但需要有效的传感平台。本文采用基于金属-有机框架的纳米酶(Ce-UiO-66/Zn)和基于碳点的纳米酶(Ce-CDs/MnO2),建立了一种新的双模式级联扩增传感策略,用于快速检测丙烯磷。由于Ce-UiO-66/Zn具有较高的类磷水解酶活性,故能快速水解丙烯磷,水解产物被具有类漆酶活性的Ce-CDs/MnO2进一步氧化,氧化产物与4-氨基安替比林偶联形成红色产物进行比色检测。此外,由于其颜色变化明显,该级联反应还被用于智能手机传感平台的探索,用于可视化分析不同食品样品中的丙诺fos,并取得了理想的回收率。该研究为高灵敏度、高选择性生物传感器的开发、食品安全和环境现场监测提供了新的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Construction of phosphatase- and laccase-mimicking Cascade catalysis for ultrasensitive colorimetric detection of Profenofos

Construction of phosphatase- and laccase-mimicking Cascade catalysis for ultrasensitive colorimetric detection of Profenofos

Construction of phosphatase- and laccase-mimicking Cascade catalysis for ultrasensitive colorimetric detection of Profenofos
Nanozyme-based dephosphorylation reactions show potential for monitoring organophosphorus pesticides (OPs) but require efficient sensing platforms. Herein, a novel dual-mode cascaded amplification sensing strategy was established for the rapid detection of profenofos by employing metal-organic framework-based nanozymes (Ce-UiO-66/Zn) and carbon-dot-based nanozymes (Ce-CDs/MnO2). Based on the high phosphohydrolase-like activity of Ce-UiO-66/Zn, profenofos could be rapidly hydrolyzed and the hydrolysis product would be further oxidized by Ce-CDs/MnO2 with laccase-like activity, and the oxidative product was coupled with 4-aminoantipyrine to form a red product for colorimetric detection. Additionally, this cascade reaction was also used in the exploration of a smartphone sensing platform due to its obvious color change, which was used to visually analyze profenofos in different food samples and achieved ideal recovery rates. This study provides new insight into development of highly sensitive and selective biosensors, food safety and environmental on-site monitoring.
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来源期刊
Food Chemistry
Food Chemistry 工程技术-食品科技
CiteScore
16.30
自引率
10.20%
发文量
3130
审稿时长
122 days
期刊介绍: Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.
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