酸解-电渗析-微生物发酵三明治一体化工艺制备的外多糖衍生低聚糖的益生元潜力

IF 6.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry: X Pub Date : 2025-07-01 DOI:10.1016/j.fochx.2025.102718

Xinmiao Ren , Qingping Liang , Mengshi Xiao , Dongxing Yu , Jiaming Liu , Ningyang Li , Xiaodan Fu , Haijin Mou

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引用次数: 0

摘要

来自人乳的浓缩寡糖（FCOs）和岩藻聚糖是潜在的益生元。本研究建立了酸解-电渗析-微生物发酵相结合的三明治整合生物工艺，从具有特定骨架结构→3)-β-d- glcp -(1→4)-α-l- fucp -(1→3)- α-l- fucp -（1→）和α-d-Galp分支的含焦外多糖（EPS）中获得新型益生元FCOs。通过单株培养和结肠发酵对FCOs的益生元活性进行了分析。EPS酸解产生残酸和游离单糖。电渗析除酸后，乳酸乳球菌发酵去除超过96%的葡萄糖和半乳糖，与最初的水解物相比，FCOs的焦点摩尔比增加了25%。二次电渗析去除96.2%的发酵源有机酸。FCOs在单培养中促进乳酸菌，在结肠发酵中增强双歧杆菌、拟副杆菌和巨孢杆菌，丰富短链脂肪酸（SCFAs）代谢途径，特别是丁酸盐代谢。本研究开发了eps衍生FCOs制备和单糖副产物去除的集成工艺，为富焦益生元的开发提供了参考。

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Prebiotic potential of exopolysaccharide-derived oligosaccharides prepared using sandwich-integration bioprocess of acidolysis-electrodialysis and microbial fermentation

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Prebiotic potential of exopolysaccharide-derived oligosaccharides prepared using sandwich-integration bioprocess of acidolysis-electrodialysis and microbial fermentation

Fucosylated oligosaccharides (FCOs) from human milk and fucoidan are potential prebiotics. This study established a sandwich-integration bioprocess combining acidolysis-electrodialysis and microbial fermentation to obtain novel prebiotic FCOs from fucose-containing exopolysaccharide (EPS) with a specific backbone structure →3)-β-d-Glcp-(1 → 4)-α-l-Fucp-(1 → 3)-α-l-Fucp-(1 → and α-d-Galp branches. The prebiotic activity of FCOs was analyzed through single-strain cultivation and colonic fermentation. Acidolysis of EPS generated residual acid and free monosaccharides. After electrodialysis for acid removal, Lactococcus lactis fermentation removed over 96 % of glucose and galactose, increasing the fucose molar ratio of FCOs by 25 % compared to the initial hydrolysates. Secondary electrodialysis removed 96.2 % of fermentation-derived organic acids. FCOs promoted lactic acid bacteria in mono-culture, and enhanced Bifidobacterium, Parabacteroides, and Megasphaera in colonic fermentation, enriching short-chain fatty acids (SCFAs) metabolism pathway, especially butyrate metabolism. This study developed an integrated process for EPS-derived FCOs preparation and monosaccharides byproduct removal and provided insights to the development of fucose-rich prebiotic.

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来源期刊

Food Chemistry: X CHEMISTRY, APPLIED-

CiteScore

4.90

自引率

6.60%

发文量

315

审稿时长

55 days

期刊介绍： Food Chemistry: X, one of three Open Access companion journals to Food Chemistry, follows the same aims, scope, and peer-review process. It focuses on papers advancing food and biochemistry or analytical methods, prioritizing research novelty. Manuscript evaluation considers novelty, scientific rigor, field advancement, and reader interest. Excluded are studies on food molecular sciences or disease cure/prevention. Topics include food component chemistry, bioactives, processing effects, additives, contaminants, and analytical methods. The journal welcome Analytical Papers addressing food microbiology, sensory aspects, and more, emphasizing new methods with robust validation and applicability to diverse foods or regions.