{"title":"水动力空化辅助细胞内聚羟基烷酸酯的回收。","authors":"Tülin Yilmaz Nayir, Yusuf Küçükağa, Serdar Kara","doi":"10.1007/s00449-025-03197-3","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, the hydrodynamic cavitation (HC) process was adopted for the recovery of intracellular biopolymer, namely polyhydroxyalkanoates (PHAs), from mixed microbial culture (MMC). To investigate the potential and performance of HC process, two cavitation devices (orifice-1 and orifice-17) were employed. The impact of biomass concentration, orifice type and pressure differential on recovery yield was assessed. The HC-assisted PHA recovery protocol introduced a novel technique that uses HC for cell disruption and a solvent for biopolymer separation. The results demonstrate the feasibility of obtaining biopolymer within a short operation time (5 min), achieving 72% process efficiency using the HC-assisted recovery procedure. The biopolymer recovered via HC at optimal conditions exhibited a purity of 71.4%, indicating effective polyhydroxybutyrate (PHB) isolation. Its molecular weight of 0.15 × 10⁶ g/mol aligns with typical PHB ranges, suggesting its suitability for various applications. Fourier-transform infrared spectroscopy (FTIR) analysis confirmed compatibility with commercial PHB. Thermal degradation profiles showed slightly lower stability compared to commercial PHB, with a 10% mass loss at 243.21 °C and a maximum degradation temperature of 262.12 °C. Despite these minor differences, HC presents a promising, greener method for PHA recovery, offering potential applications in sustainable industries.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hydrodynamic cavitation assisted recovery of intracellular polyhydroxyalkanoates.\",\"authors\":\"Tülin Yilmaz Nayir, Yusuf Küçükağa, Serdar Kara\",\"doi\":\"10.1007/s00449-025-03197-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In this study, the hydrodynamic cavitation (HC) process was adopted for the recovery of intracellular biopolymer, namely polyhydroxyalkanoates (PHAs), from mixed microbial culture (MMC). To investigate the potential and performance of HC process, two cavitation devices (orifice-1 and orifice-17) were employed. The impact of biomass concentration, orifice type and pressure differential on recovery yield was assessed. The HC-assisted PHA recovery protocol introduced a novel technique that uses HC for cell disruption and a solvent for biopolymer separation. The results demonstrate the feasibility of obtaining biopolymer within a short operation time (5 min), achieving 72% process efficiency using the HC-assisted recovery procedure. The biopolymer recovered via HC at optimal conditions exhibited a purity of 71.4%, indicating effective polyhydroxybutyrate (PHB) isolation. Its molecular weight of 0.15 × 10⁶ g/mol aligns with typical PHB ranges, suggesting its suitability for various applications. Fourier-transform infrared spectroscopy (FTIR) analysis confirmed compatibility with commercial PHB. Thermal degradation profiles showed slightly lower stability compared to commercial PHB, with a 10% mass loss at 243.21 °C and a maximum degradation temperature of 262.12 °C. Despite these minor differences, HC presents a promising, greener method for PHA recovery, offering potential applications in sustainable industries.</p>\",\"PeriodicalId\":9024,\"journal\":{\"name\":\"Bioprocess and Biosystems Engineering\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-07-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioprocess and Biosystems Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s00449-025-03197-3\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprocess and Biosystems Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s00449-025-03197-3","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Hydrodynamic cavitation assisted recovery of intracellular polyhydroxyalkanoates.
In this study, the hydrodynamic cavitation (HC) process was adopted for the recovery of intracellular biopolymer, namely polyhydroxyalkanoates (PHAs), from mixed microbial culture (MMC). To investigate the potential and performance of HC process, two cavitation devices (orifice-1 and orifice-17) were employed. The impact of biomass concentration, orifice type and pressure differential on recovery yield was assessed. The HC-assisted PHA recovery protocol introduced a novel technique that uses HC for cell disruption and a solvent for biopolymer separation. The results demonstrate the feasibility of obtaining biopolymer within a short operation time (5 min), achieving 72% process efficiency using the HC-assisted recovery procedure. The biopolymer recovered via HC at optimal conditions exhibited a purity of 71.4%, indicating effective polyhydroxybutyrate (PHB) isolation. Its molecular weight of 0.15 × 10⁶ g/mol aligns with typical PHB ranges, suggesting its suitability for various applications. Fourier-transform infrared spectroscopy (FTIR) analysis confirmed compatibility with commercial PHB. Thermal degradation profiles showed slightly lower stability compared to commercial PHB, with a 10% mass loss at 243.21 °C and a maximum degradation temperature of 262.12 °C. Despite these minor differences, HC presents a promising, greener method for PHA recovery, offering potential applications in sustainable industries.
期刊介绍：
Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes.
Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged.
The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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