Qi Zhu, Xu Sun, Yu Du, Xiaoyue Zhang, Xiang Ren, Hongmin Ma, Dan Wu, Huangxian Ju, Qin Wei
{"title":"基于原子精密双金属纳米簇Au3Ag5（MSA）的多肽可切割分裂型传感器检测基质金属蛋白酶2","authors":"Qi Zhu, Xu Sun, Yu Du, Xiaoyue Zhang, Xiang Ren, Hongmin Ma, Dan Wu, Huangxian Ju, Qin Wei","doi":"10.1021/acs.analchem.5c02007","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, a split-type electrochemiluminescence (ECL) sensor based on polypeptide cleavage was proposed for the sensitive detection of matrix metalloproteinase 2 (MMP 2). The bimetallic nanoclusters were protected by mercaptosuccinic acid (MSA), named Au<sub>3</sub>Ag<sub>5</sub>(MSA)<sub>3</sub>, serving as a signal probe. Owing to the synergistic effect of the bimetal composition, the clusters exhibited enhanced ECL properties. Polypeptide chains (PLGVR) that can be specifically cleaved by MMP 2 were selected from the MMP 2 domain. Additionally, the antifouling segment (PPEKEK) and the binding segment (CCC) were incorporated into the polypeptide chain design. In the presence of MMP 2, a specific cleavage site on the polypeptide was targeted, resulting in the detachment of Au<sub>3</sub>Ag<sub>5</sub>(MSA)<sub>3</sub> and subsequent generation of an ECL signal. Compared to conventional detection methods, this approach exhibited enhanced sensitivity and improved stability. This method provides a valuable reference for detecting members of the matrix metalloproteinase family and holds promising prospects for future development.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":"14574-14583"},"PeriodicalIF":6.7000,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of Matrix Metalloproteinase 2 by a Polypeptide Cleavable Split-Type Sensor Based on Atomically Precise Bimetallic Nanocluster Au<sub>3</sub>Ag<sub>5</sub>(MSA)<sub>3</sub>.\",\"authors\":\"Qi Zhu, Xu Sun, Yu Du, Xiaoyue Zhang, Xiang Ren, Hongmin Ma, Dan Wu, Huangxian Ju, Qin Wei\",\"doi\":\"10.1021/acs.analchem.5c02007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In this study, a split-type electrochemiluminescence (ECL) sensor based on polypeptide cleavage was proposed for the sensitive detection of matrix metalloproteinase 2 (MMP 2). The bimetallic nanoclusters were protected by mercaptosuccinic acid (MSA), named Au<sub>3</sub>Ag<sub>5</sub>(MSA)<sub>3</sub>, serving as a signal probe. Owing to the synergistic effect of the bimetal composition, the clusters exhibited enhanced ECL properties. Polypeptide chains (PLGVR) that can be specifically cleaved by MMP 2 were selected from the MMP 2 domain. Additionally, the antifouling segment (PPEKEK) and the binding segment (CCC) were incorporated into the polypeptide chain design. In the presence of MMP 2, a specific cleavage site on the polypeptide was targeted, resulting in the detachment of Au<sub>3</sub>Ag<sub>5</sub>(MSA)<sub>3</sub> and subsequent generation of an ECL signal. Compared to conventional detection methods, this approach exhibited enhanced sensitivity and improved stability. This method provides a valuable reference for detecting members of the matrix metalloproteinase family and holds promising prospects for future development.</p>\",\"PeriodicalId\":27,\"journal\":{\"name\":\"Analytical Chemistry\",\"volume\":\" \",\"pages\":\"14574-14583\"},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2025-07-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.analchem.5c02007\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/7/4 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.5c02007","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/4 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Detection of Matrix Metalloproteinase 2 by a Polypeptide Cleavable Split-Type Sensor Based on Atomically Precise Bimetallic Nanocluster Au3Ag5(MSA)3.
In this study, a split-type electrochemiluminescence (ECL) sensor based on polypeptide cleavage was proposed for the sensitive detection of matrix metalloproteinase 2 (MMP 2). The bimetallic nanoclusters were protected by mercaptosuccinic acid (MSA), named Au3Ag5(MSA)3, serving as a signal probe. Owing to the synergistic effect of the bimetal composition, the clusters exhibited enhanced ECL properties. Polypeptide chains (PLGVR) that can be specifically cleaved by MMP 2 were selected from the MMP 2 domain. Additionally, the antifouling segment (PPEKEK) and the binding segment (CCC) were incorporated into the polypeptide chain design. In the presence of MMP 2, a specific cleavage site on the polypeptide was targeted, resulting in the detachment of Au3Ag5(MSA)3 and subsequent generation of an ECL signal. Compared to conventional detection methods, this approach exhibited enhanced sensitivity and improved stability. This method provides a valuable reference for detecting members of the matrix metalloproteinase family and holds promising prospects for future development.
期刊介绍：
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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